SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet

doi:10.1016/j.jia.2024.12.002

Journal of Integrative Agriculture

2025, Vol. 24

Issue (6): 2438-2442 DOI: 10.1016/j.jia.2024.12.002

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SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet

Yongchao Li¹, Mengmeng Sun¹, Rui Zhao¹, Jiayi Chen¹, Yunhao Chen¹, Shuqi Dong^{1, 2}, Xiangyang Yuan^{1, 2}, Xiaorui Li^{1, 2}, Lulu Gao¹, Guanghui Yang¹, Peiyong Xin³, Shujing Cheng³, Jinfang Chu^{3, 4}, Xiaoqian Chu^1#, Jiagang Wang^1#

¹Houji Laboratory in Shanxi Province/College of Agriculture, Shanxi Agricultural University, Taigu 030801, China

²State Key Laboratory of Sustainable Dryland Agriculture (in Preparation), Shanxi Agricultural University, Taigu 030801, China

³National Centre for Plant Gene Research (Beijing)/Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

⁴University of Chinese Academy of Sciences, Beijing 100049, China

Highlights

● Phenotype of dwarf4 is due to the abnormal intersegmental elongation and reduced gibberellin synthesis.
● SiDWARF4 encodes the ent-copalyl diphosphate synthase (CPS) enzyme involved in gibberellin (GA) biosynthesis in foxtail millet.

Abstract
References

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摘要

赤霉素在植物生长发育中起着重要作用。作物矮化主要与赤霉素合成或信号转导途径的突变有关。然而，对作物中与赤霉素合成和调控有关的基因知之甚少。本研究分离到一株极端矮化的自然矮杆突变体dwarf4。基于BSA-seq，证实了由SiDWARF4编码的古巴焦磷酸合成酶（CPS）在谷子GA生物合成过程中催化了牻牛儿基牻牛儿基焦磷酸（GGPP）向古巴焦磷酸（CPP）的转化。在dwarf4中，CPS酶底物GGPP水平升高，导致GAs含量降低。外源GA₃处理可以恢复dwarf4的异常表型。SiDWARF4敲除系表型与dwarf4一致。与对照Ci846相比，GGPP含量升高，GAs含量降低，与dwarf4的结果一致。研究结果对改良谷子株型和提高谷子种植密度具有一定的指导意义。

Received: 04 September 2024 Online: 04 December 2024 Accepted: 24 October 2024

Fund:

This work was supported by the National Natural Science Foundation of China (32200222), the High-level Talents Start-up Fund of Shanxi Agricultural University, China (J242198006), the Start-up Fund of Shanxi Agricultural University, China (2021BQ84), the Shanxi Province Outstanding Doctoral and Post-Doctoral Scholarship Award Foundation, China (SXBYKY2021055 and SXBYKY2022033), and the Houji Laboratory Foundation, China (202204010910001-32).

About author: #Correspondence Jiagang Wang, E-mail: wjg@sxau.edu.cn; Xiaoqian Chu, E-mail: chuxiaoqian@sxau.edu.cn

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Yongchao Li, Mengmeng Sun, Rui Zhao, Jiayi Chen, Yunhao Chen, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Lulu Gao, Guanghui Yang, Peiyong Xin, Shujing Cheng, Jinfang Chu, Xiaoqian Chu, Jiagang Wang. 2025. SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet. Journal of Integrative Agriculture, 24(6): 2438-2442.

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