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Journal of Integrative Agriculture  2024, Vol. 23 Issue (4): 1274-1284    DOI: 10.1016/j.jia.2023.10.003
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Characterization of Domeless receptors and the role of BdDomeless3 in anti-symbiont-like virus defense in Bactrocera dorsalis

Wei Zhang1, 2, Shaoyang Li1, Rong Li1, Jinzhi Niu1, 2, Jinjun Wang1, 2#

1 Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715,     China

2 Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education/International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China

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摘要  

JAK/STATJanus kinase/signal transducers and activators of transcription是无脊椎动物重要的先天免疫信号通路,细胞因子受体Domeless是其上游通路的核心组分分析发现,桔小实蝇有3Domeless受体(BdDomeless1BdDomeless2BdDomeless3),均具有5个纤连蛋白(FN Ⅲ)胞外结构域和1个跨膜结构域,且在成虫期高表达,并对病原物侵染具有一定诱导响应进一步分析发现,仅BdDomelss3在关键免疫组织脂肪体中显著高表达,并能够响应类共生病毒的侵染;RNAi靶向BdDomeless3可促进Bactrocera dorsalis cripavirus(BdCV)Bactrocera dorsalis picorna-like virus(BdPLV)复制说明细胞因子受体BdDomeless3参与抵抗桔小实蝇类共生病毒复制过程,暗示JAK/STAT通路在桔小实蝇免疫系统中具有重要作用本研究首次对实蝇类昆虫JAK/STAT通路细胞因子受体进行了表征,并探究了BdDomeless3的免疫功能,为揭示桔小实蝇的先天免疫机制提供了新见解。



Abstract  

The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway play a pivotal role in innate immunity.  Among invertebrates, Domeless receptors serve as the key upstream regulators of this pathway.  In our study on Bactrocera dorsalis, we identified three cytokine receptors: BdDomeless1, BdDomeless2, and BdDomeless3.  Each receptor encompasses five fibronectin-type-III-like (FN III) extracellular domains and a transmembrane domain.  Furthermore, these receptors exhibit the increased responsiveness to diverse pathogenic challenges.  Notably, only BdDomeless3 is upregulated during symbiont-like viral infections.  Moreover, silencing BdDomeless3 enhanced the infectivity of Bactrocera dorsalis cripavirus (BdCV) and B. dorsalis picorna-like virus (BdPLV), underscoring BdDomeless3’s crucial role in antiviral defense of B. dorsalis.  Following the suppression of Domeless3 expression, six antimicrobial peptide genes displayed decreased expression, potentially correlating with the rise in viral infectivity.  To our knowledge, this is the first study identifying cytokine receptors associated with the JAK/STAT pathway in tephritid flies, shedding light on the immune mechanisms of B. dorsalis.

Keywords:  Bactrocera dorsalis       JAK/STAT pathway        Domeless receptors        antiviral immunity        symbiont-like virus   
Received: 06 April 2023   Accepted: 22 August 2023
Fund: This work was supported by the National Natural Science Foundation of China (32202278) and the Chongqing Special Postdoctoral Science Foundation of China, and the earmarked fund for China Agricultural Research System (CARS-26).
About author:  Wei Zhang, Tel: +86-23-68250653, E-mail: zhangwei1995@swu.edu.cn; #Correspondence Jinjun Wang, Tel: +86-23-68250255, E-mail: wangjinjun@swu.edu.cn

Cite this article: 

Wei Zhang, Shaoyang Li, Rong Li, Jinzhi Niu, Jinjun Wang. 2024. Characterization of Domeless receptors and the role of BdDomeless3 in anti-symbiont-like virus defense in Bactrocera dorsalis. Journal of Integrative Agriculture, 23(4): 1274-1284.

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