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Journal of Integrative Agriculture
Special Issue: 园艺作物基因功能与分子调控机制Horticulture — Gene function · Molecular mechanism
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Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17

XU Min1, 2, GAO Zhao1, 2, LI Da-long1, ZHANG Chen1, 2, ZHANG Yu-qi1, 2, HE Qian1, 2, QI Ying-bin1, 2, ZHANG He1, 2, JIANG Jing-bin1, 2, XU Xiang-yang1, 2#, ZHAO Ting-ting1, 2#

1 College of Horticulture and Landscape ArchitectureNortheast Agricultural University, Harbin 150030, P.R.China

2 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, P.R.China

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Abstract  

PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress. However, there has been very little research on the function of this family gene in tomatoes, which limits its application in germplasm resource improvement. Therefore, the PLATZ gene family was identified and analyzed in tomato, and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function. In this study, the PLATZ family members of tomato were identified in the whole genome, and 19 SlPLATZ genes were obtained. Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis. SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources. SlPLATZ17 was selected for functional verification by experiment-based analysis. The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants. Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long, thin petals, adjacent petals were not connected at the base, and the stamen circumference was smaller. This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.

Keywords:  Tomato              PLATZ family              abiotic stresses              plant development  
Online: 03 August 2023  
Fund: 

The China Agriculture Research System (CARS-23-A11); the Heilongjiang Provincial Natural Science Foundation of China (YQ2021C013); the National Natural Science Foundation of China (32102390); the National Natural Science Foundation of China (32072589); Northeast Agricultural University Scholars Program (20XG28).

About author:  #Correspondence XU Xiang-yang, Tel: +8613009869477, E-mail: xxy709@126.com

Cite this article: 

XU Min, GAO Zhao, LI Da-long, ZHANG Chen, ZHANG Yu-qi, HE Qian, QI Ying-bin, ZHANG He, JIANG Jing-bin, XU Xiang-yang, ZHAO Ting-ting. 2023. Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17. Journal of Integrative Agriculture, Doi:doi.org/10.1016/j.jia.2023.08.003

Bailey T L, Boden M, Buske F A, Frith M, Grant C E, Clementi L, Ren J, Li W W, Noble W S. 2009. MEME SUITE: tools for motif discovery and searching. Nucleic Acids Research37, W202-W208.

Bakshi M, Oelmuller R. 2014. WRKY transcription factors: Jack of many trades in plants. Plant Signaling & Behavior9, e27700.

Beyer Jr W F, Fridovich I. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical Biochemistry161, 559-566.

Cervilla L M, Blasco B, Ríos J J, Romero L, Ruiz J M. 2007. Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Annals of Botany100, 747-756.

Chance B, Maehly A C. 1955. Assay of catalases and peroxidases. Methods in Enzymology. 2, 764-775.

Chao Q, Gao Z F, Zhang D, Zhao B G, Dong F Q, Fu C X, Wang B C. 2019. The developmental dynamics of the Populus stem transcriptome. Plant Biotechnology Journal17, 206-219.

Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant13, 1194-1202.

Ciftci-Yilmaz S, Mittler R. 2008. The zinc finger network of plants. Cellular and Molecular Life Sciences, 65, 1150-1160. 

Claussen W. 2005. Proline as a measure of stress in tomato plants. Plant Science168, 241-248.

Eck J V, Kirk D D, Walmsley A M. 2006. Tomato (Lycopersicum esculentum). Agrobacterium Protocols, 343, 459-474.

Fernandez-Pozo N, Menda N, Edwards J D, Saha S, Tecle I Y, Strickler S R, Bombarely A, Fisher-York T, Pujar A, Foerster H, Yan A, Mueller L A. 2015. The Sol Genomics Network (SGN)—from genotype to phenotype to breeding. Nucleic Acids Research43, D1036-D1041.

González-Morales S I, Chávez-Montes R A, Hayano-Kanashiro C, Alejo-Jacuinde G, Rico-Cambron T Y, de Folter S, Herrera-Estrella L. 2016. Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thalianaProceedings of the National Academy of Sciences113, E5232-E5241.

Grellet Bournonville C F, Díaz-Ricci J C. 2011. Quantitative determination of superoxide in plant leaves using a modified NBT staining method. Phytochemical Analysis22, 268-271.

Hande A S, Katageri I S, Jadhav M P, Adiger S, Gamanagatti S, Padmalatha K V, Dhandapani G, Kanakachari M, Kumar P A, Reddy V S. 2017. Transcript profiling of genes expressed during fibre development in diploid cotton (Gossypium arboreum L.). BMC Genomics18, 1-15.

Honma T, Goto K. 2001. Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature, 409, 525-529.

Horton P, Park K J, Obayashi T, Fujita N, Harada H, Adams-Collier C J, Nakai K. 2007. WoLF PSORT: protein localization predictor. Nucleic Acids Research35, W585-W587.

Huang D, Wu W, Abrams S R, Cutler A J. 2008. The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. Journal of Experimental Botany59, 2991-3007.

Huang H, Chen Y, Wang S, Qi T, Song S. 2023. Jasmonate action and crosstalk in flower development and fertility. Journal of Experimental Botany, 74, 1186-1197.

Kasirajan L, Hoang N V, Furtado A, Botha F C, Henry R J. 2018. Transcriptome analysis highlights key differentially expressed genes involved in cellulose and lignin biosynthesis of sugarcane genotypes varying in fiber content. Scientific Reports8, 11612.

Kielbowicz-Matuk A. 2012. Involvement of plant C (2)H(2)-type zinc finger transcription factors in stress responses. Plant Science, 185, 78-85.

Kim J H, Kim J, Jun S E, Park S, Timilsina R, Kwon D S, Woo H R. 2018. ORESARA15, a PLATZ transcription factor, mediates leaf growth and senescence in Arabidopsis. New Phytologist220, 609-623.

Kou X, Wang S, Wu M, Guo R, Xue Z, Meng N, Tao X, Chen M, Zhang Y. 2014. Molecular Characterization and Expression Analysis of NAC Family Transcription Factors in Tomato. Plant Molecular Biol Reporter. 32, 501-516.

Kumari A, Kumar J, Kumar A, Chaudhury A, Singh S P. 2015. Grafting triggers differential responses between scion and rootstock. PLoS One10, e0124438.

Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S. 2002. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research30, 325-327.

Li X, He F, Zhao G, Li M, Long R, Kang J, Yang Q, Chen L. 2023. Genome-Wide Identification and Phylogenetic and Expression Analyses of the PLATZ Gene Family in Medicago sativa L. International Journal of Molecular Sciences24, 2388.

Liu S, Yang R, Liu M, Zhang S, Yan K, Yang G, Wu C. 2020. PLATZ2 negatively regulates salt tolerance in Arabidopsis seedlings by directly suppressing the expression of the CBL4/SOS3 and CBL10/SCaBP8 genes. Journal of Experimental Botany71, 5589-5602.

Liu X, Dai Y, Li R, Yuan L, Chen X, Wang X. 2019. Members of B-box Protein Family from Malus domestica Enhanced Abiotic Stresses Tolerance in Escherichia coli. Molecular Biotechnology61, 421-426.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods, 25, 402-408.

Ma X, Liang W, Gu P, Huang Z. 2016. Salt tolerance function of the novel C2H2-type zinc finger protein TaZNF in wheat. Plant Physiology and Biochemistry, 106, 129-140.

Martin C, Paz-Ares J. 1997. MYB transcription factors in plants. Trends in Genetics13, 67-73.

Nagano Y, Furuhashi H, Inaba T, Sasaki Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Research, 29, 4097-4105.

Nantel A, Quatrano R S. 1996. Characterization of three rice basic/leucine zipper factors, including two inhibitors of EmBP-1 DNA binding activity. Journal of Biological Chemistry271, 31296-31305.

Ni X, Tian Z, Liu J, Song B, Xie C. 2010. Cloning and molecular characterization of the potato RING finger protein gene StRFP1 and its function in potato broad-spectrum resistance against Phytophthora infestans. Journal of Plant Physiology, 167, 488-496.

Noman A, Liu Z, Aqeel M, Zainab M, Khan M I, Hussain A, He S. 2017. Basic leucine zipper domain transcription factors: the vanguards in plant immunity. Biotechnology Letters39, 1779-1791.

Raikhel N. 1992. Nuclear targeting in plants. Plant physiology, 100, 1627-1632.

Ramel F, Sulmon C, Bogard M, Couée I, Gouesbet G. 2009. Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets. BMC Plant Biology9, 1-18.

Ramsby M L, Makowski G S. 2005. Differential detergent fractionation of eukaryotic cells. The Proteomics Protocols Handbook, 10.1385/1-59259-890-0:037.

Riechmann J L, Meyerowitz E M. 1998. The AP2/EREBP family of plant transcription factors. Biological Chemistry379, 633-646.

Saegusa A. 1999. Japanese plan speeds up rice genome sequencing. Nature, 401, 102-102.

Sato S, Tabata S, Hirakawa H, Asamizu E, Shirasawa K, Isobe S, Fernandez-Munoz R. 2012. The tomato genome sequence provides insights into fleshy fruit evolution. Nature485, 635-641.

Sirko A, Anna Wawrzyńska, Brzywczy J, Marzena Sieńko. 2021. Control of aba signaling and crosstalk with other hormones by the selective degradation of pathway components. International Journal of Molecular Sciences, 22, 4638.

Su D, Xiang W, Wen L, Lu W, Shi Y, Liu Y, Li Z. 2021. Genome-wide identification, characterization and expression analysis of BES1 gene family in tomato. BMC Plant Biology21, 1-17.

Takatsuji H. 1998. Zinc-finger transcription factors in plants. Cellular and Molecular Life Sciences CMLS54, 582-596.

Velásquez A C, Chakravarthy S, Martin G B. 2009. Virus-induced gene silencing (VIGS) in Nicotiana benthamiana and tomato. JoVE (Journal of Visualized Experiments), 28, e1292.

Wang A, Hou Q, Si L, Huang X, Luo J, Lu D, Han B. 2019. The PLATZ transcription factor GL6 affects grain length and number in rice. Plant Physiology180, 2077-2090.

Wang J, Ji C, Li Q, Zhou Y, Wu Y. 2018. Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex. BMC Plant Biology18, 1-12.

Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J H, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires J C, Paterson A H, Chalhoub B, Wang B, et al. 2011. The genome of the mesopolyploid crop species Brassica rapaNature Genetics43, 1035-1039.

Yan A, Wu M, Zhao Y, Zhang A, Liu B, Schiefelbein J, Gan Y. 2014. Involvement of C2H2 Zinc finger proteins in the regulation of epidermal cell fate determination in Arabidopsis. Journal of Integrative Plant Biology, 12, 1112-1117.

Yoshida, Mogami, Yamaguchi-Shinozaki. 2015. Omics approaches toward defining the comprehensive abscisic acid signaling network in plants. Plant Cell Physiology, 56, 1043-1052.

Zang D, Wang C, Ji X, Wang Y. 2015. Tamarix hispida zinc finger protein ThZFP1 participates in salt and osmotic stress tolerance by increasing proline content and SOD and POD activities. Plant Science, 235, 111-121.

Zenda T, Liu S, Wang X, Liu G, Jin H, Dong A, Duan H. 2019. Key maize drought-responsive genes and pathways revealed by comparative transcriptome and physiological analyses of contrasting inbred lines. International Journal of Molecular Sciences20, 1268.

Zhang C Y, Long Y, Feng J, Meng J L. 2007. Transcriptional regulation of plant genes and its significance in biology. Yi Chuan= Hereditas29, 793-799.

Zhang S, Yang R, Huo Y, Liu S, Yang G, Huang J, Zheng C, Wu C. 2018. Expression of cotton PLATZ1 in transgenic Arabidopsis reduces sensitivity to osmotic and salt stress for germination and seedling establishment associated with modification of the abscisic acid, gibberellin, and ethylene signalling pathways. BMC Plant Biology18, 1-11.

Zhao J, Zheng L, Wei J, Wang Y, Chen J, Zhou Y, Chen M, Wang F, Ma Y, Xu Z S. 2022. The soybean PLATZ transcription factor GmPLATZ17 suppresses drought tolerance by interfering with stress-associated gene regulation of GmDREB5. The Crop Journal10, 1014-1025.

Zhou S R, Xue H W. 2020. The rice PLATZ protein SHORT GRAIN6 determines grain size by regulating spikelet hull cell division. Journal of Integrative Plant Biology62, 847-864.

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