Please wait a minute...
Journal of Integrative Agriculture  2023, Vol. 22 Issue (8): 2521-2535    DOI: 10.1016/j.jia.2023.02.023
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Soil conditioners improve Cd-contaminated farmland soil microbial communities to inhibit Cd accumulation in rice
ZHAO Jun-yang1, 2, LU Hua-ming1, QIN Shu-tao1, PAN Peng1, TANG Shi-de1, CHEN Li-hong1, WANG Xue-li1, TANG Fang-yu3, TAN Zheng-long1, WEN Rong-hui1, 2#, HE Bing1#

1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, P.R.China

2 College of Life Science and Technolegy, Guangxi University, Nanning 530004, P.R.China

3 Guangxi Liyuanbao Science and Technology Co., Ltd., Guangxi Zhuang Autonomous Region, Nanning 530004, P.R.China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

土壤调理剂中添加硅和有机肥可抑制重金属离子从土壤向作物的转移,但目前并不清楚硅和有机肥如何通过影响土壤性质和微生态环境进而减少水稻的镉积累。本研究通过两地田间实验分析了含硅和有机肥的L型土壤调理剂对细菌和真菌群落多样性、土壤pH、有机质及有效硅含量的影响。结果表明,随着土壤调理剂中硅和有机肥含量的增加,两个试验点的水稻产量分别增加了16.8%~25.8%6.8%~13.1%, 稻米Cd含量显著降低了8.2%~21.1%10.8%~40.6%。土壤微生物组学分析证明与镉吸附、螯合相关的FirmicutesActinobacteriota细菌和与大分子物质降解有关的Basidiomycota真菌种群丰度的增加,有利于抑制土壤中镉的活性(土壤ESC-Cd降低了14.4%~14.8%18.1%~20.6%),这与施用土壤调理剂引起有机质和有效硅含量增加有关。总之,L型土壤调理剂中的有机质和硅可通过调节土壤中镉钝化优势菌群,降低土壤镉有效性,最终减少稻米镉积累。



Abstract  The addition of silicon (Si) and organic fertilizers to soil conditioners can inhibit the transfer of heavy metal ions from soil to crops. However, it is not clear how Si and organic fertilizers affect soil properties and the micro-ecological environment and thereby reduce cadmium (Cd) accumulation in rice. In this study, the effects of L-type soil conditioners containing Si and organic fertilizers on bacterial and fungal community diversity, soil pH, organic matter, and available Si were analyzed with field experiments at two sites in Liuzhou City and Hezhou City, respectively, in Guangxi, China. With the increase of Si and organic fertilizer content in soil conditioner, rice yield respectively increased by 16.8–25.8 and 6.8–13.1%, and rice Cd content decreased significantly by 8.2–21.1 and 10.8–40.6%, respectively, at the two experimental sites. Soil microbiome analysis showed that the increase in abundance of Firmicutes and Actinobacteriota bacteria associated with Cd adsorption and sequestration, and Basidiomycota fungal populations associated with degradation of macromolecules favored the inhibition of soil Cd activity (soil exchangeable Cd decreased by 14.4–14.8 and 18.1–20.6%). This was associated with an increase in organic matter and Si content caused by applying soil conditioners. In conclusion, L-type soil conditioners, rich in Si and organic fertilizer, can reduce soil Cd bioavailability by regulating the dominant Cd passivating flora in the soil and ultimately reduce Cd accumulation in rice.
Keywords:  soil conditioner        silicon        organic matter        microbial community        Cd accumulation in rice  
Received: 16 September 2022   Accepted: 07 December 2022
Fund: This work was supported by the National Natural Science Foundation of China (31560122) and the Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, China.
About author:  ZHAO Jun-yang, E-mail: 761174981@qq.com; # Correspondence WEN Rong-hui, E-mail: wenrh@gxu.edu.cn; HE Bing, E-mail: hebing@gxu.edu.cn

Cite this article: 

ZHAO Jun-yang, LU Hua-ming, QIN Shu-tao, PAN Peng, TANG Shi-de, CHEN Li-hong, WANG Xue-li, TANG Fang-yu, TAN Zheng-long, WEN Rong-hui, HE Bing. 2023. Soil conditioners improve Cd-contaminated farmland soil microbial communities to inhibit Cd accumulation in rice. Journal of Integrative Agriculture, 22(8): 2521-2535.

Bao S D. 2000. Soil and Agricultural Chemistry Analysis. China Agricultural Press, Beijing. (in Chinese)

Chen X W, Wu L, Luo N, Mo C H, Wong M H, Li H. 2019. Arbuscular mycorrhizal fungi and the associated bacterial community influence the uptake of cadmium in rice. Geoderma337, 749–757.

Cheng Z Y, Shi J C, He Y, Wu L S, Xu J M. 2022. Assembly of root-associated bacterial community in cadmium contaminated soil following five-year consecutive application of soil amendments: Evidences for improved soil health. Journal of Hazardous Materials426, 128095.

Dhami N K, Reddy M S, Mukherjee A. 2013. Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites. Journal of Microbiology and Biotechnology23, 707–714.

Ding Z L, Kheir A M S, Ali O A M, Hafez E M, ElShamey E A, Zhou Z X, Wang B Z, Lin X, Ge Y, Fahmy A E, Seleiman M F. 2021. A vermicompost and deep tillage system to improve saline-sodic soil quality and wheat productivity. Journal of Environmental Management277, 111388.

GB/T 23739-2009. 2009. Soil quality - Analysis of available lead and cadmium contents in soils - Atomic absorption spectrometry. Ministry of Agriculture of the People’s Republic of China. pp. 1–5. (in Chinese)

Govarthanan M, Mythili R, Selvankumar T, Kamala-Kannan S, Rajasekar A, Chang Y C. 2016. Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. RM isolated from the roots of Tridax procumbens. 3 Biotech6, 242.

Han X M, Hu H W, Chen Q L, Yang L Y, Li H L, Zhu Y G, Li X Z, Ma Y B. 2018. Antibiotic resistance genes and associated bacterial communities in agricultural soils amended with different sources of animal manures. Soil Biology and Biochemistry126, 91–102.

Huang Y, Sheng H, Zhou P, Zhang Y. 2019. Remediation of Cd-contaminated acidic paddy fields with four-year consecutive liming. Ecotoxicology and Environmental Safety188, 109903.

Koski-Vähälä J, Hartikainen H, Tallberg P. 2001. Phosphorus mobilization from various sediment pools in response to increased pH and silicate concentration. Journal of Environmental Quality30, 546–552.

Li B, Yang L, Wang C Q, Zheng S Q, Xiao R, Guo Y. 2019. Effects of organic-inorganic amendments on the cadmium fraction in soil and its accumulation in rice (Oryza sativa L.). Environmental Science and Pollution Research26, 13762–13772.

Li H B, Zhang H Q, Yang Y J, Fu G F, Tao L X, Xiong J. 2022. Effects and oxygen-regulated mechanisms of water management on cadmium (Cd) accumulation in rice (Oryza sativa). Science of the Total Environment846, 157484.

Li J Y, Zhang J, Ma Y H, Cai W D, Gao F. 2018. Effect of silicon on cadmium subcellular distribution and non protein sulfhydryl content of two rice varieties under cadmium stress. Journal of Agricultural Environmental Sciences37, 1066–1071. (in Chinese)

Li M, Cheng X H, Guo H X. 2013. Heavy metal removal by biomineralization of urease producing bacteria isolated from soil. International Biodeterioration & Biodegradation76, 81–85.

Li P F, Liu M, Ma X Y, Wu M, Jiang C Y, Liu K, Liu J, Li Z P. 2020. Responses of microbial communities to a gradient of pig manure amendment in red paddy soils. Science of the Total Environment705, 135884.

Li Y C, Li Y F, Chang S X, Liang X, Qin H, Chen J H, Xu Q F. 2017. Linking soil fungal community structure and function to soil organic carbon chemical composition in intensively managed subtropical bamboo forests. Soil Biology and Biochemistry107, 19–31.

Li Z M, Unzué-Belmonte D, Cornelis J T, Linden C V, Struyf E, Ronsse F, Delvaux B. 2019. Effects of phytolithic rice-straw biochar, soil buffering capacity and pH on silicon bioavailability. Plant and Soil438, 187–203.

Liang Y C, Wong J W C, Wei L. 2005. Silicon-mediated enhancement of cadmium tolerance in maize (Zea mays L.) grown in cadmium contaminated soil. Chemosphere58, 475–483.

Lin H, Sun W C, Zhang Z L, Chapman S J, Freitag T E, Fu J R, Zhang X, Ma J W. 2016. Effects of manure and mineral fertilization strategies on soil antibiotic resistance gene levels and microbial community in a paddy-upland rotation system. Environmental Pollution211, 332–337.

Lin W P, Jiang N H, Peng L, Fan X Y, Gao Y, Wang G P, Cai K Z. 2020. Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation. Journal of Integrative Agriculture19, 251–264.

Liu C S, Zhao D F, Ma W J, Guo Y D, Wang A J, Wang Q L, Lee D J. 2016. Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp. Applied Microbiology and Biotechnology100, 1421–1426.

Liu H X, Yang Y J, Yang Y Z, Zhong X B, Lv J L. 2022. Dynamics of fungal and bacterial communities in different types of soil ageing with different dosages of cadmium. Ecotoxicology and Environmental Safety242, 113860.

Lopes A R, Manaia C M, Nunes O C. 2014. Bacterial community variations in an alfalfa–rice rotation system revealed by 16S rRNA gene 454-pyrosequencing. FEMS Microbiology Ecology87, 650–663.

Luo G W, Rensing C, Chen H, Liu M Q, Wang M, Guo S W, Ling N, Shen Q R. 2018. Deciphering the associations between soil microbial diversity and ecosystem multifunctionality driven by long-term fertilization management. Functional Ecology32, 1103–1116.

Ma C Y, Ci K D, Zhu J, Sun Z L, Liu Z X, Li X Y, Zhu Y L, Tang C, Wang P, Liu Z M. 2021. Impacts of exogenous mineral silicon on cadmium migration and transformation in the soil–rice system and on soil health. Science of the Total Environment759, 143501.

Ministry of Environmental Protection (MEP), China. 2014. The national soil pollution survey bulletin. [2022-10-11]. http://www.mep.gov.cn/gkml/hbb/qt/201404/t20140417_270670.htm (in Chinese)

Narendrula-Kotha R, Nkongolo K K. 2017. Bacterial and fungal community structure and diversity in a mining region under long-term metal exposure revealed by metagenomics sequencing. Ecological Genetics and Genomics2, 13–24.

Ning D F, Liang Y C, Liu Z D, Xiao J F, Duan A W. 2016. Impacts of steel-slag-based silicate fertilizer on soil acidity and silicon availability and metals-immobilization in a paddy soil. PLoS ONE11, e0168163.

NY 525–2021. 2021. Organic Fertilizer. Agricultural Industry Standards of the People’s Republic of China, China. (in Chinese)

Qian X Y, Fang C L, Huang M S, Achal V. 2017. Characterization of fungal-mediated carbonate precipitation in the biomineralization of chromate and lead from an aqueous solution and soil. Journal of Cleaner Production164, 198–208.

Rathod J, Dhanani A S, Jean J S, Jiang W. 2019. The whole genome insight on condition-specific redox activity and arsenopyrite interaction promoting As-mobilization by strain Lysinibacillus sp. B2A1. Journal of Hazardous Materials364, 671–681.

Saengwilai P, Meeinkuirt W, Phusantisampan T, Pichtel J. 2020. Immobilization of cadmium in contaminated soil using organic amendments and its effects on rice growth performance. Exposure and Health12, 295–306.

Seleiman M F, Ali S, Refay Y, Rizwan M, Alhammad B A, El-Hendawy S E. 2020. Chromium resistant microbes and melatonin reduced Cr uptake and toxicity, improved physio-biochemical traits and yield of wheat in contaminated soil. Chemosphere250, 126239.

Sheng H, Gu Y, Yin Z R, Xue Y, Zhou P, Thompson M L. 2022. Consistent inter-annual reduction of rice cadmium in 5-year biannual organic amendment. Science of the Total Environment807, 151026.

Shi T R, Ma J, Wu X , Ju T N, Lin X L, Zhang Y Y, Li X H, Gong Y W, Hou H, Zhao L, Wu F Y. 2018. Inventories of heavy metal inputs and outputs to and from agricultural soils: A review. Ecotoxicology and Environmental Safety164, 118–124.

Smith A P, Marín-Spiotta E, De Graaff M A, Balser T C. 2014. Microbial community structure varies across soil organic matter aggregate pools during tropical land cover change. Soil Biology and Biochemistry77, 292–303.

Song A, Li Z M, Wang E Z, Xu D Y, Wang S, Bi J J, Wang H L, Jeyakumar P, Li Z Y, Fan F L. 2021. Supplying silicon alters microbial community and reduces soil cadmium bioavailability to promote health wheat growth and yield. Science of the Total Environment796, 148797.

Suvendu D, Jeong G L, Song R C, Hyeon J S, Pil J K. 2019. Silicate fertilizer amendment alters fungal communities and accelerates soil organic matter decomposition. Frontiers in Microbiology10, 2950–2950.

Tang J Y, Zhang J C, Ren L H, Zhou Y Y, Gao J, Luo L, Yang Y, Peng Q H, Huang H L, Chen A W. 2019. Diagnosis of soil contamination using microbiological indices: A review on heavy metal pollution. Journal of Environmental Management242, 121–130.

Tessier A, Campbell P G C, Bisson M. 1979. Sequential extraction procedure for the speciation of particulate trace metals. Analytical Chemistry51, 844–851.

Venegas A, Rigol A, Vidal M. 2015. Viability of organic wastes and biochars as amendments for the remediation of heavy metal-contaminated soils. Chemosphere119, 190–198.

Wang B H, Chu C B, Wei H W, Zhang L M, Ahmad Z, Wu S H, Xie B. 2020. Ameliorative effects of silicon fertilizer on soil bacterial community and pakchoi (Brassica chinensis L.) grown on soil contaminated with multiple heavy metals. Environmental Pollution267, 115411.

Wang F, Zhang W W, Miao L J, Ji T W, Wang Y F, Zhang H J, Ding Y, Zhu W Q. 2021. The effects of vermicompost and shell powder addition on Cd bioavailability, enzyme activity and bacterial community in Cd-contaminated soil: A field study. Ecotoxicology and Environmental Safety215, 112163.

Wang M Z, Liu P, Xiong W G, Zhou Q, Wangxiao J Y, Zeng Z L, Sun Y X. 2018. Fate of potential indicator antimicrobial resistance genes (ARGS) and bacterial community diversity in simulated manure-soil microcosms. Ecotoxicology & Environmental Safety147, 817–823.

Wang M, Li S S, Chen S B, Meng N, Li X Y, Zheng H, Zhao C M, Wang D. 2019. Manipulation of the rhizosphere bacterial community by biofertilizers is associated with mitigation of cadmium phytotoxicity. Science of the Total Environment649, 413–421.

Wei X, Liu Y Q, Zhan Q, Zhang P B, Zhao D D, Xu B, Bocharnikova E, Matichenkov V. 2017. Effect of Si soil amendments on As, Cd, and Pb bioavailability in contaminated paddy soils. Paddy and Water Environment16, 173–181.

Wei X, Zhang P B, Zhao D D, Bochanikova E, Matichenkov V, Dmitry D. 2018. Effect of silicon application in paddy soil on cadmium reduction in soil rice system. Acta Ecologica Sinica38, 1600–1606. (in Chinese)

Xiao Z X, Peng M, Mei Y C, Tan L, Liang Y C. 2021. Effect of organosilicone and mineral silicon fertilizers on chemical forms of cadmium and lead in soil and their accumulation in rice. Environmental Pollution283, 117107.

Yu Y, Wan Y Y, Camara A Y, Li H F. 2018. Effects of the addition and aging of humic acid-based amendments on the solubility of Cd in soil solution and its accumulation in rice. Chemosphere196, 303–310.

Zhang D, Ding A F. 2019. Effects of passivating agents on the availability of Cd and Pb and microbial community function in a contaminated acidic soil. Bulletin of Environmental Contamination and Toxicology103, 98–105.

Zhang L W, Shang Z B, Guo K X, Chang Z X, Liu H L, Li D L. 2019. Speciation analysis and speciation transformation of heavy metal ions in passivation process with thiol-functionalized nano-silica. Chemical Engineering Journal369, 979–987.

Zhao J Y, Yu B S, Wang X L, Chen L H, Akhtar K, Tang S D, Lu H M, He J H, Wen R H, He B. 2022. Differences in the response mechanism of cadmium uptake, transfer and accumulation of different rice varieties to foliar silicon spraying under Cd stress soil. Frontiers in Plant Science13, 5428.

Zhao J, Wang P, Yu X F, Shu W, Xu Q Y. 2019. Structure and composition of sediment-associated bacterial and eukaryotic communities in the river-lake system of Poyang Lake, China. Geomicrobiology Journal36, 727–736.

Zhao Y T, Liu M D, Guo L, Yang D, He N, Ying B, Wang Y J. 2020. Influence of silicon on cadmium availability and cadmium uptake by rice in acid and alkaline paddy soils. Journal of Soils and Sediments, 20, 2343–2353.

Zhou J, Zhang C, Du B, Cui H B, Fan X J, Zhou D M, Zhou J. 2021. Soil and foliar applications of silicon and selenium effects on cadmium accumulation and plant growth by modulation of antioxidant system and Cd translocation: Comparison of soft vs. durum wheat varieties. Journal of Hazardous Materials402, 123546.

Zhou L J, Wu L, Lin X B, Wang H M, Liu H, Zhang H Y, Hu M, Shi L P, Zhang Y, Huang Q R. 2019. Remediation effect of soil conditioners on cadmium-contaminated paddy fields. Environmental Science40, 9. (in Chinese)

No related articles found!
No Suggested Reading articles found!