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Journal of Integrative Agriculture  2022, Vol. 21 Issue (7): 2019-2030    DOI: 10.1016/S2095-3119(21)63855-8
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Characterization of laccase gene StLAC6 involved in the pathogenicity and peroxisome function in Setosphaeria turcica

LIU Ning1 ,2*, ZHANG Qian-qian3*, JIA Hui1, ZHAO Bin1,2ZHU Zi-ping4 , CAO Zhi-yan1, 2, DONG Jin-gao1, 2

1 State Key Laboratory of North China Crop Improvement and Regulation / Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071001, P.R.China

2 College of Plant Protection, Hebei Agricultural University, Baoding 071001, P.R.China

3 College of Life Sciences, Hebei Agriculture University, Baoding 071001, P.R.China

4 Langfang Academy of Agriculture and Forestry Science, Langfang 065000, P.R.China

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Laccases, as a kind of multicopper oxidase, play an important role in pigment synthesis and growth in fungi and are involved in their interactions with host plants.  In Setosphaeria turcica, 9 laccase-like multicopper oxidases have been identified, and StLAC2 is involved in the synthesis of the melanin that accumulates in the cell wall.  The function of another major laccase gene, StLAC6, was studied here.  The knockout of StLAC6 had no effect on the growth, morphology or invasion ability of S. turcica, but the morphology and function of peroxisomes of knockout mutants were abnormal.  The knockout of the StLAC6 gene resulted in increased contents of phenolic compounds and melanin and the sensitivity to fungicides increased compared with wild type strains.  In the mutants of StLAC6, there is a significant change of the expression levels of other laccase genes.  This study provides a new insight into laccase functions and the relationship of the laccase gene family in plant pathogenic fungi.   

Keywords:  Setosphaeria turcica        Laccase       StLAC6       peroxisome       fungicides  
Received: 21 June 2021   Accepted: 29 October 2021
Fund: This work was supported by the grants from the National Natural Science Foundation of China (31901827), the China Agriculture Research System (CARS-02), the Natural Science Foundation of Hebei Province, China (C2020204039), the Key Research and Development Projects of Hebei Province (20326510D), and the Hebei Province Projects of Overseas Foundation (C20190508).
About author:  LIU Ning, E-mail:; Correspondence CAO Zhi-yan, Tel: +86-312-7528142, E-mail:; DONG Jin-gao, Tel: +86-312-7528166, E-mail: * These authors contributed equally to this study.

Cite this article: 

LIU Ning, ZHANG Qian-qian, JIA Hui, ZHAO Bin, ZHU Zi-ping , CAO Zhi-yan, DONG Jin-gao. 2022. Characterization of laccase gene StLAC6 involved in the pathogenicity and peroxisome function in Setosphaeria turcica. Journal of Integrative Agriculture, 21(7): 2019-2030.

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