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Journal of Integrative Agriculture  2019, Vol. 18 Issue (11): 2598-2604    DOI: 10.1016/S2095-3119(19)62739-5
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome
ZHANG Jun-qin1, 2*, WEI Yan-ming1, 2, 3*, HUANG Kun1, 2, SUN Xiao-mei1, 2, 3, ZOU Zhong1, 2, 3, JIN Mei-lin1, 2, 3
1 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P.R.China
2 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P.R.China
3 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, P.R.China
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Hepatitis-hydropericardium syndrome (HHS) is an infectious disease caused by fowl adenovirus serotype 4 (FAdV-4).  Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines.  However, the protective efficiency of baculovirus-expressed penton base protein has not been assessed.  In this study, two recombinant capsid proteins, penton base and fiber-2, were constructed.  And then, penton base and fiber-2 were administrated alone or together to specific pathogen-free (SPF) chickens at 14 days of life and boosted at 28 days of life.  At 42 days of life, the immunized groups and the control group were challenged with FAdV-4 virulent strain.  Results show that inoculating penton base or penton base+fiber-2 provided 100% protection to the chickens.  All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions.  Thus, baculovirus-expressed penton base protein is a promising candidate subunit vaccine.
Keywords:  fowl adenovirus serotype 4        hepatitis-hydropericardium syndrome        penton base        subunit vaccine        baculovirus expression system  
Received: 29 December 2018   Accepted:
Fund: This research was supported by the National Key Research and Development Program of China (2016YFD0500801). 
Corresponding Authors:  Correspondence JIN Mei-lin, E-mail:; ZOU Zhong, E-mail:    
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHANG Jun-qin, WEI Yan-ming, HUANG Kun, SUN Xiao-mei, ZOU Zhong, JIN Mei-lin . 2019.

Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome
. Journal of Integrative Agriculture, 18(11): 2598-2604.

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