农业生态环境-有机碳与农业废弃物还田合辑Agro-ecosystem & Environment—SOC
|Integrated management of crop residue and nutrients enhances new carbon formation by regulating microbial taxa and enzymes
|WU Hong-liang1, CAI An-dong2, XING Ting-ting1, HUAI Sheng-chang1, ZHU Ping3, HAN Xiao-zeng4, XU Ming-gang5, LU Chang-ai1
|1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081, P.R.China
2 Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3 Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R.China
4 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R.China
5 College of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, P.R.China
本研究通过84天的室内培养试验来揭示秸秆和养分（氮(N)、磷(P)和硫(S)）联合供应下土壤新碳生成的潜在微生物机制。结果表明，与对照土壤相比，单独添加秸秆刺激微生物进行养分开采，这与C:N和C:P酶活的比例降低了8-16%相吻合。随着养分补充水平的增提高，公主岭土壤新碳生成量从1155.9增加到1722.4 mg kg-1，海伦土壤则从725.1增加到1067.5 mg kg-1。回归树分析表明β-葡萄糖苷酶（BG）、酸性磷酸酶（AP）、微生物量碳（MBC）和酸杆菌对公主岭新碳生成的相对影响分别为27.8、18.5、14.7和8.1%；对海伦新碳生成的相对影响分别为25.9、29.5、10.1和13.9%。路径分析表明酸杆菌通过调节BG、AP和MBC直接或间接对土壤新碳生成产生积极影响，其中MBC的获取更多受到AP的调节。海伦土壤的新碳生成强度低于公主岭土壤，并且海伦土壤的新碳生成与AP活性直接相关，表明土壤属性（例如SOC和pH值）对土壤新碳生成的重要性。综上，本研究揭示了添加秸秆的土壤中新碳生成与NPS养分补充的响应关系，且土壤新碳生成主要依赖于酸杆菌和变形菌的生长代谢及对BG和AP的调控。
Abstract Although returning crop residue to fields is a recommended measure for improving soil carbon (C) stocks in agroecosystems, the response of newly formed soil C (NFC) to the integrated supply of residue and nutrients and the microbial mechanisms have not been fully understood. Therefore, an 84-day incubation experiment was conducted to ascertain the microbial mechanisms that underpin the NFC response to inputs of residue and nitrogen (N), phosphorus (P), and sulfur (S) in two black soils. The results showed that adding residue alone accelerated microbial nutrient mining, which was supported by decreases of 8–16% in the ratios of C:N and C:P enzyme activities (relative to soils with nutrient inputs). The NFC amounts increased from 1155.9 to 1722.4 mg kg−1 soil in Gongzhuling and increased from 725.1 to 1067.5 mg kg−1 soil in Hailun as the levels of nutrient supplementation increased. Boosted regression tree analysis suggested that β-glucosidase (BG), acid phosphatase (AP), microbial biomass C (MBC), and Acidobacteria accounted for 27.8, 18.5, 14.7, and 8.1%, respectively, of the NFC in Gongzhuling and accounted for 25.9, 29.5, 10.1, and 13.9%, respectively, of the NFC in Hailun. Path analysis determined that Acidobacteria positively influenced NFC both directly and indirectly by regulating BG, AP, and MBC, in which MBC acquisition was regulated more by AP. The intensity of NFC was lower in Hailun soil than in Gongzhuling soil and was directly affected by AP, thereby indicating the importance of soil status (e.g., SOC and pH) in determining NFC. Overall, our results reveal the response of NFC to supplementation by N, P, and S, which depends on Acidobacteria and Proteobacteria, and their investment in BG and AP in residue-amended soil.
Received: 23 February 2021
Accepted: 24 May 2021
|Fund: This work was financially supported by the Agro-scientific Research in the Public Interest of China (201503122), the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-XTCX2016008), and the National Natural Science Foundation of China (41620104006).
|About author: WU Hong-liang, E-mail: firstname.lastname@example.org; Correspondence LU Chang-ai, Tel/Fax: +86-10-82108703, E-mail: email@example.com
Cite this article:
WU Hong-liang, CAI An-dong, XING Ting-ting, HUAI Sheng-chang, ZHU Ping, HAN Xiao-zeng, XU Ming-gang, LU Chang-ai.
Integrated management of crop residue and nutrients enhances new carbon formation by regulating microbial taxa and enzymes. Journal of Integrative Agriculture, 21(6): 1772-1785.
| An T T, Schaeffer S, Zhuang J, Radosevich M, Li S Y, Li H, Pei J B, Wang J K. 2015. Dynamics and distribution of 13C-labeled straw carbon by microorganisms as affected by soil fertility level in the Black Soil region of Northeast China. Biology and Fertility of Soils, 51, 605–613.
Banerjee S, Kirkby C A, Schmutter D, Bissett A, Kirkegaard J A, Richardson A E. 2016. Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biology and Biochemistry, 97, 188–198.
Blagodatskaya E, Kuzyakov Y. 2008. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: Critical review. Biology and Fertility of Soils, 45, 115–131.
Brooks A N, Turkarslan S, Beer K D, Yin L F, Baliga N S. 2011. Adaptation of cells to new environments. Wiley Interdisciplinary Reviews (Systems Biology and Medicine), 3, 544–561.
Cai A D, Liang G P, Zhang X B, Zhang W J, Li L, Rui Y C, Xu M G, Luo Y Q. 2018. Long-term straw decomposition in agro-ecosystems described by a unified three-exponentiation equation with thermal time. Science of the Total Environment, 636, 699–708.
Chen R R, Senbayram M, Blagodatsky S, Myachina O, Dittert K, Lin X G, Blagodatskaya E, Kuzyakov Y. 2014. Soil C and N availability determine the priming effect: Microbial N mining and stoichiometric decomposition theories. Global Change Biology, 20, 2356–2367.
Creamer C A, Jones D L, Baldock J A, Rui Y C, Murphy D V, Hoyle F C, Farrell M. 2016. Is the fate of glucose-derived carbon more strongly driven by nutrient availability, soil texture, or microbial biomass size? Soil Biology and Biochemistry, 103, 201–212.
Creamer C A, Menezes A B, Krull E S, Sanderman J, Newton-Walters R, Farrell M. 2015. Microbial community structure mediates response of soil C decomposition to litter addition and warming. Soil Biology and Biochemistry, 80, 175–188.
Ding J, Jiang X, Ma M, Zhou B, Li J. 2016. Effect of 35 years inorganic fertilizer and manure amendment on structure of bacterial and archaeal communities in black soil of Northeast China. Applied Soil Ecology, 105, 187–195.
Elith J, Leathwick J R, Hastie T. 2008. A working guide to boosted regression trees. Journal of Animal Ecology, 77, 802–813.
Fang Y Y, Nazaries L, Singh B K, Singh B P. 2018. Microbial mechanisms of carbon priming effects revealed during the interaction of crop residue and nutrient inputs in contrasting soils. Global Change Biology, 24, 2775–2790.
FAO. 1985. FAO/Uncsco Soil Map of the World. 1:5,000,000, Revised legend. FAO, Rome.
Fatemi F R, Fernandez I J, Simon K S, Dail D B. 2016. Nitrogen and phosphorus regulation of soil enzyme activities in acid forest soils. Soil Biology and Biochemistry, 98, 171–179.
Fontaine S, Mariotti A, Abbadie L. 2003. The priming effect of organic matter: A question of microbial competition? Soil Biology and Biochemistry, 35, 837–843.
Foster E J, Hansen N, Wallenstein M, Cotrufo M F. 2016. Biochar and manure amendments impact soil nutrients and microbial enzymatic activities in a semi-arid irrigated maize cropping system. Agriculture, Ecosystems & Environment, 233, 404–414.
Gulis V, Suberkropp K. 2003. Leaf litter decomposition and microbial activity in nutrient-enriched and unaltered reaches of a headwater stream. Freshwater Biology, 48, 123–134.
Han P, Zhang W, Wang G, Sun W, Huang Y. 2016. Changes in soil organic carbon in croplands subjected to fertilizer management: A global meta-analysis. Scientific Reports, 6, 27199.
Han X, Xu C, Dungait J A J, Bol R, Wang X J, Wu W L, Meng F Q. 2018. Straw incorporation increases crop yield and soil organic carbon sequestration but varies under different natural conditions and farming practices in China: A system analysis. Biogeosciences Discussions, 15, 1933–1946.
Heuck C, Weig A, Spohn M. 2015. Soil microbial biomass C:N:P stoichiometry and microbial use of organic phosphorus. Soil Biology and Biochemistry, 85, 119–129.
Hou D D, Wang K, Liu T, Wang H X, Lin Z, Qian J, Lu L L, Tian S K. 2017. Unique rhizosphere micro-characteristics facilitate phytoextraction of multiple metals in soil by the hyperaccumulating plant Sedum alfredii. Environmental Science & Technology, 51, 5675–5684.
Hu S J, Van Bruggen A H C, Grunwald N J. 1999. Dynamics of bacterial populations in relation to carbon availability in a residue-amended soil. Applied Soil Ecology, 13, 21–30.
Huang Y, Liang C, Duan X W, Chen H, Li D J. 2019. Variation of microbial residue contribution to soil organic carbon sequestration following land use change in a subtropical karst region. Geoderma, 353, 340–346.
Jian S Y, Li J W, Chen J, Wang G S, Mayes M A, Dzantor K E, Hui D F, Luo Y Q. 2016. Soil extracellular enzyme activities, soil carbon and nitrogen storage under nitrogen fertilization: A meta-analysis. Soil Biology and Biochemistry, 101, 32–43.
Kaiser C, Franklin O, Dieckmann U, Richter A. 2014. Microbial community dynamics alleviate stoichiometric constraints during litter decay. Ecology Letters, 17, 680–690.
Kirkby C A, Richardson A E, Wade L J, Batten G D, Blanchard C, Kirkegaard J A. 2013. Carbon-nutrient stoichiometry to increase soil carbon sequestration. Soil Biology and Biochemistry, 60, 77–86.
Kirkby C A, Richardson A E, Wade L J, Passioura J B, Batten G D, Blanchard C, Kirkegaard J A. 2014. Nutrient availability limits carbon sequestration in arable soils. Soil Biology and Biochemistry, 68, 402–409.
Kõljalg U, Nilsson R H, Abarenkov K, Tedersoo L, Taylor A F S, Bahram M, Bates S T, Larsson K H. 2013. Towards a unified paradigm for sequence-based identification of fungi. Molecular Ecology, 22, 5271–5277.
Lal R. 2008. Carbon sequestration. Philosophical Transactions of the Royal Society (B: Biological Sciences), 363, 815–830.
Liang C, Balser T C. 2011. Microbial production of recalcitrant organic matter in global soils: Implications for productivity and climate policy. Nature Reviews Microbiology, 9, 75.
Liu Y W, Feng Y, Cheng D M, Xue J M, Wakelin S A, Hu H Y, Li Z J. 2017. Gentamicin degradation and changes in fungal diversity and physicochemical properties during composting of gentamicin production residue. Bioresource Technology, 244, 905–912.
Manzoni S, Trofymow J A, Jackson R B, Porporato A. 2010. Stoichiometric controls on carbon, nitrogen, and phosphorus dynamics in decomposing litter. Ecological Monographs, 80, 89–106.
Marschner P, Hatam Z, Cavagnaro T R. 2015. Soil respiration, microbial biomass and nutrient availability after the second amendment are influenced by legacy effects of prior residue addition. Soil Biology and Biochemistry, 88, 169–177.
Miltner A, Kindler R, Knicker H, Richnow H, Kastner M. 2009. Fate of microbial biomass-derived amino acids in soil and their contribution to soil organic matter. Organic Geochemistry, 40, 978–985.
Olsen S R, Sommers L E. 1982. Phosphorus. In: Page A L, Miller R H, Keeney D R, eds., Methods of Soil Analysis. Chemical and Microbiological Properties. vol. 2. American Society of Agronomy, Madison. pp. 403–430.
Powlson D S, Glendining M J, Coleman K, Whitmore A P. 2011. Implications for soil properties of removing cereal straw: Results from long-term studies. Agronomy Journal, 103, 279–287.
Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner F O. 2013. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Research, 41, 590–596.
Ramirez K S, Craine J M, Fierer N. 2012. Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes. Global Change Biology, 18, 1918–1927.
Saiya-Cork K R, Sinsabaugh R L, Zakb D R. 2002. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 34, 1309–1315.
Sarker J R, Singh B P, Cowie A L, Fang Y, Collins D, Dougherty W J, Singh B K. 2018. Carbon and nutrient mineralization dynamics in aggregate-size classes from different tillage systems after input of canola and wheat residues. Soil Biology and Biochemistry, 116, 22–38.
Song Z, Liu H, Li B, Yang X. 2013. The production of phytolith-occluded carbon in China’s forests: Implications to biogeochemical carbon sequestration. Global Change Biology, 19, 2907–2915.
Stocka S C, Köster M, Dippold M A, Nájera F, Matus F, Merino C, Boy J, Spielvogel S, Gorbushina A, Kuzyakov Y. 2019. Environmental drivers and stoichiometric constraints on enzyme activities in soils from rhizosphere to continental scale. Geoderma, 337, 973–982.
Stockmann U, Adams M A, Crawford J W, Field D J, Henakaarchchi N, Jenkins M, Wheeler I. 2013. The knowns, known unknowns and unknowns of sequestration of soil organic carbon. Agriculture, Ecosystems & Environment, 164, 80–99.
Tian P, Razavi B S, Zhang X C, Wang Q K, Blagodatskaya E. 2020. Microbial growth and enzyme kinetics in rhizosphere hotspots are modulated by soil organics and nutrient availability. Soil Biology and Biochemistry, 141, 107662.
Vance E, Brookes P C, Jenkinson D S. 1987. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 19, 703–707.
Wang S C, Zhao Y W, Wang J Z, Zhu P, Cui X, Han X Z, Xu M G, Lu C A. 2018. The efficiency of long-term straw return to sequester organic carbon in Northeast China’s cropland. Journal of Integrative Agriculture, 17, 436–448.
Wang X Y, Yu D S, Wang C, Pan Y, Pan J J, Shi X Z. 2018. Variations in cropland soil organic carbon fractions in the black soil region of China. Soil Tillage Research, 184, 93–99.
Waring B G, Weintraub S R, Sinsabaugh R L. 2014. Ecoenzymatic stoichiometry of microbial nutrient acquisition in tropical soils. Biogeochemistry, 117, 101–113.
Wei X M, Zhu Z K, Liu Y, Luo Y, Deng Y W, Xu X L, Liu S L, Richter A, Shibistova O, Guggenberger G, Wu J S, Ge T. 2020. C:N:P stoichiometry regulates soil organic carbon mineralization and concomitant shifts in microbial community composition in paddy soil. Biology and Fertility of Soils, 56, 1093–1107.
Wu J, Joergensen R G, Pommerening B, Chaussod R, Brookes P C. 1990. Measurement of soil microbial biomass C by fumigation extraction - An automated procedure. Soil Biology and Biochemistry, 22, 1167–1169.
Xu Z W, Yu G R, Zhang X Y, He N P, Wang Q F, Wang S Z, Wang R L, Zhao N, Jia Y L, Wang C Y. 2017. Soil enzyme activity and stoichiometry in forest ecosystems along the North–South Transect in eastern China (NSTEC). Soil Biology and Biochemistry, 104, 152–163.
Yan X Y, Cai Z C, Wang S W, Smith P. 2011. Direct measurement of soil organic carbon content change in the croplands of China. Global Change Biology, 17, 1487–1496.
Yin C, Fan F, Song A, Cui P, Li T, Liang Y. 2015. Denitrification potential under different fertilization regimes is closely coupled with changes in the denitrifying community in a black soil. Applied Microbiology and Biotechnology, 99, 5719–5729.
Zhao Y C, Wang M Y, Hu S J, Zhang X D, Ouyang Z, Zhang Z L, Huang B, Zhao S W, Wu J S, Xie D T, Zhu B, Yu D S, Pan X Z, Xu S X, Shi X Z. 2018. Economics- and policy-driven organic carbon input enhancement dominates soil organic carbon accumulation in Chinese croplands. Proceedings of the National Academy of Sciences of the United States of America, 115, 4045–4050.
Zhou J, Bing H, Wu Y, Yang Z, Wang J, Sun H, Luo J, Liang J. 2016. Rapid weathering processes of a 120-year-old chronosequence in the Hailuogou Glacier foreland, Mt. Gongga, SW China. Geoderma, 267, 78–91.
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