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Journal of Integrative Agriculture  2022, Vol. 21 Issue (3): 774-780    DOI: 10.1016/S2095-3119(21)63618-3
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Incidence and prevalence levels of three aphid-transmitted viruses in crucifer crops in China
ZHANG Xiao-yan1, 2, PENG Yan-mei1, XIANG Hai-ying1, WANG Ying1, LI Da-wei1, YU Jia-lin1, HAN Cheng-gui1 
1 State Key Laboratory for Agrobiotechnology, Ministry of Agriculture and Rural Affairs/Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, P.R.China
2 College of Agriculture, Ludong University, Yantai 264025, P.R.China
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马铃薯卷叶病毒属病毒在世界范围内广泛分布,能够侵染多种经济作物引起严重病害。芸薹黄化病毒(Brassica yellows virus, BrYV)是在我国鉴定出的一种马铃薯卷叶病毒属的新病毒,主要侵染十字花科作物。该病毒主要包含三种基因型,分别为A、B和C,目前关于该种病毒在我国的发生分布情况尚不清楚,本研究主要对芸薹黄化病毒三种基因型在我国十字花科作物上的发生分布情况进行调查。2014-2018年,对采自我国29个省(自治区、直辖市)的570份十字花科作物的样品进行RT-PCR检测。结果表明:共有97份样品检测到BrYV侵染,病毒平均发生率为17.0%。共有22个省(自治区、直辖市)的样品中检测到BrYV的侵染,其中在我国的华北地区、西北地区和东北地区有相对较高的病毒发生率。进一步利用多重RT-PCR检测方法对BrYV的三种基因型进行检测,在97份BrYV侵染的样品中:61.9%的样品受单种基因型的侵染,29.9%的样品受两种基因型的复合侵染,8.2%的样品受三种基因型的复合侵染;A、B和C三种基因型的检出率分别为39.2%、40.2%和67.0%。以上研究表明,BrYV在我国的十字花科作物上发生分布比较普遍,北方地区要比南方地区各省病毒发生率高。BrYV发生存在单基因型侵染和多种基因型复合侵染的现象,C基因型具有相对较高的发生率,为优势基因型。并且本研究还发现BrYV与其它两种蚜传病毒芜菁花叶病毒(Turnip mosaic virus, TuMV)和黄瓜花叶病毒(Cucumber mosaic virus, CMV)在十字花科作物上普遍发生复合侵染的现象。本研究首次对BrYV在我国的十字花科作物上的发生分布情况展开大量调查,研究结果将有助于该病毒病害的有效防控。

Poleroviruses, which are distributed worldwide, infect many crops of economic importance and cause severe plant diseases.  Brassica yellows virus (BrYV), which has three genotypes, A, B, and C, is a newly identified polerovirus infecting crucifer crops in China, but its distribution is still unclear.  Here, we report the distribution and prevalence levels of the three BrYV genotypes in crucifer crops in China.  A total of 570 crucifer leaf samples randomly collected from 22 provinces, four ethnic minority autonomous regions, and three municipalities in China were tested for BrYV.  RT-PCR detection showed that 97 of the field samples were positive for BrYV, and the average incidence of BrYV was 17.0%.  The virus was detected in 22 provinces, with high incidences in north, northwest, and northeast China.  The multiplex RT-PCR amplification of the three BrYV genotypes revealed that both single and mixed infections occurred.  Among the BrYV infections, 38.1% were mix-infected by more than two viral genotypes, and 8.2% samples were mix-infected by three viral genotypes.  Our findings indicated a widespread prevalence of BrYV in China, and BrYV mixed infections with Turnip mosaic virus and Cucumber mosaic virus in crucifer crops are common.  This study is the first large-scale survey of BrYV in crucifer crops in China.  The information generated in this investigation will contribute to the national prevention and control of viral diseases.
Keywords:  Brassica yellows virus        three genotypes        incidence        crucifer crops  
Received: 11 September 2020   Accepted: 05 January 2021
Fund: This work was supported in part by the National Natural Science Foundation of China (31900139 and 31671995) and the Key R&D Project of Shandong Province (2019GSF107095).  
Corresponding Authors:  Correspondence HAN Cheng-gui, Tel: +86-10-62733336, Fax: +86-10-62813785, E-mail:   
About author:  ZHANG Xiao-yan, E-mail:; Correspondence HAN Cheng-gui, Tel: +86-10-62733336, Fax: +86-10-62813785, E-mail:

Cite this article: 

ZHANG Xiao-yan, PENG Yan-mei, XIANG Hai-ying, WANG Ying, LI Da-wei, YU Jia-lin, HAN Cheng-gui. 2022. Incidence and prevalence levels of three aphid-transmitted viruses in crucifer crops in China. Journal of Integrative Agriculture, 21(3): 774-780.

Congdon B S, Baulch J R, Coutts B A. 2020. Impact of Turnip yellows virus infection on seed yield of an open-pollinated and hybrid canola cultivar when inoculated at different growth stages. Virus Research, 277, 197847.
Fukuzawa N, Itchoda N, Ishihara T, Goto K, Masuta C, Matsumura T. 2010. HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants. Virus Genes, 40, 440–446.
Han C G, Li D W, Xing Y M, Zhu K, Tian Z F, Cai Z N, Yu J L, Liu Y. 2000. Wheat yellow mosaic virus widely occurring in wheat (Triticum aestivum) in China. Plant Disease, 84, 627–630.
Kamitani M, Nagano A J, Honjo M N, Kudoh H. 2016. RNA-Seq reveals virus–virus and virus–plant interactions in nature. FEMS Microbiology Ecology, 92, 1–11.
Li Y, Sun Q, Zhao T, Xiang H, Zhang X, Wu Z, Zhou C, Zhang X, Wang Y, Zhang Y, Wang X, Li D, Yu J, Dinesh-Kumar S P, Han C. 2019. Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways. New Phytologist, 222, 1458–1473.
Lim S, Yoo R H, Igori D, Zhao F, Kim K H, Moon J S. 2015. Genome sequence of a recombinant brassica yellows virus infecting Chinese cabbage. Archives of Virology, 160, 597–600.
Liu Y, Li F, Li Y Y, Zhang S B, Gao X W, Xie Y, Yan F, Zhang A S, Dai L Y, Cheng Z B, Ding M, Niu Y B, Wang S J, Che H Y, Jiang T, Shi X B, He Z F, Wu Y F, Zhang D Y, Qing L, et al. 2018. Identification, distribution and occurrence of viruses in the main vegetables of China. Scientia Agricultura Sinica, 52, 239–261. (in Chinese)
Palukaitis P, García-Arenal F. 2003. Cucumoviruses. Advances in Virus Research, 62, 241–323.
Pruss G, Ge X, Shi X M, Carrington J C, Bowman Vance V. 1997. Plant viral synergism: The potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses. Plant Cell, 9, 859–868.
Stevens M, Freeman B, Liu H Y, Herrbach E, Lemaire O. 2005. Beet poleroviruses: Close friends or distant relatives? Molecular Plant Pathology, 6, 1–9.
Syller J. 2012. Facilitative and antagonistic interactions between plant viruses in mixed infections. Molecular Plant Pathology, 13, 204–216.
Tian C L, Ye S H, Li S R, Chen X, Luo K, Xiang S Y, Wang L, Xie Z Y, Dong P, Qing L, Sun X C. 2019. Molecular identification of pathogens of Brassica juncea var. gemmifera and Brassica juncea var. tumida virus disease in Chongqing. Acta Horticulturae Sinica, 46, 738–748. (in Chinese)
Tomlinson J. 2008. Epidemiology and control of virus diseases of vegetables. Annals of Applied Biology, 110, 661–681.
Walsh J A, Jenner C E. 2002. Turnip mosaic virus and the quest for durable resistance. Molecular Plant Pathology, 3, 289–300.
Wang Q, Mao J J, Xiang H Y, Dong L H, Sun Y H, Liu G S, Liu H B. 2015. First report of Brassica yellows virus on tobacco in China. Plant Disease, 99, 1192.
Xiang H Y, Dong S W, Shang Q X, Zhou C J, Li D W, Yu J L, Han C G. 2011. Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China. Archives of Virology, 156, 2251–2255.
Yoshida N, Tamada T. 2019. Host range and molecular analysis of Beet leaf yellowing virus, Beet western yellows virus-JP and Brassica yellows virus in Japan. Plant Pathology, 68, 1045–1058.
Zhang X, Peng Y, Wang Y, Zhang Z, Li D, Yu J, Han C. 2016. Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction. Virology Journal, 13, 189.
Zhang X Y, Dong S W, Xiang H Y, Chen X R, Li D W, Yu J L, Han C G. 2015. Development of three full-length infectious cDNA clones of distinct brassica yellows virus genotypes for agrobacterium-mediated inoculation. Virus Research, 197, 13–16.
Zhang X Y, Xiang H Y, Zhou C J, Li D W, Yu J L, Han C G. 2014. Complete genome sequence analysis identifies a new genotype of brassica yellows virus that infects cabbage and radish in China. Archives of Virology, 159, 2177–2180.
Zhang X Y, Zhao T Y, Li Y Y, Xiang H Y, Dong S W, Zhang Z Y, Wang Y, Li D W, Yu J L, Han C G. 2018. The conserved proline18 in the polerovirus P3a is important for brassica yellows virus systemic infection. Frontiers in Microbiology, 9, 613.
Zhou C J, Zhang X Y, Liu S Y, Wang Y, Li D W, Yu J L, Han C G. 2017. Synergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana. Scientific Report, 7, 45132.

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