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Journal of Integrative Agriculture  2019, Vol. 18 Issue (8): 1871-1881    DOI: 10.1016/S2095-3119(18)62146-X
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Effect of nanosilver (nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions
Marcelina Krupa-Ma?kiewicz1, Jan Oszmiański2, Sabina Lachowicz2, Ma?gorzata Szczepanek3, Bogus?awa Ja?kiewicz4, Kamila Pachnowska5, Ireneusz Ochmian5 
1 Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology Szczecin, Szczecin 71-434, Poland; ORCID 0000-0002-4333-9122
2 Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wroclaw University of Environmental and Life Sciences, Wroc?aw 51-630, Poland; Sabina Lachowicz ORCID 0000-0001-6182-0211
3 Department of Agrotechnology, UTP University of Science and Technology, Bydgoszcz 85-225, Poland
4 Departament of Cereal Crop Production, Institute of Soil Science and Plant Cultivation National Research Institute, Pu?awy 24-100, Poland
5 Department of Horticulture, West Pomeranian University of Technology Szczecin, Szczecin 71-434, Poland; ORCID 0000-0002-3606-1927
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Cereals, including barley, have been playing a key role in human diet for a long time.  The objective of the present study was to determine the effect of nanosilver (nAg) on limitation of infections, morphological features, and their chemical composition of young barley seedlings under in vitro condition.  Addition of 0, 2, 4, 6 and 8 mg dm–3 nAg into MS medium was used.  Obtained results showed that the effect on the morphological features depended on the nAg concentration.  The addition of 6 and 8 mg dm–3 nAg into MS medium limited the number of infected barley embryos in vitro, whereas 4 and 8 mg dm–3 nAg resulted in the highest seedlings with the longest roots.  nAg in the medium affected the colour of leaves and increased the contents of chlorophyll and β-carotene, in particular in seedlings growing in MS medium supplied with 6 mg dm–3 nAg.  The addition of 8 mg dm–3 had the greatest effect on the contents of vitamin C and E in young barley seedlings.  It was found that the contents of K and Ca in the young barley leaves were lower, as compared to control plants.  The presence of 6 mg dm–3 nAg in the medium resulted in an increased contents of N, Mg, Zn, Cu, and P.  Hence, a diversified effect of nAg on individual groups of polyphenolic compounds was noticed.  The presence of 2 and 8 mg dm–3 nAg caused higher content values of polyphenolic compounds in young barley leaves.
Keywords:  polyphenols        mineral compositions        micro propagation        contaminations        Hordeum vulgare  
Received: 15 August 2018   Accepted:
Fund: This work was supported by the grant of West Pomeranian University of Technology in Szczecin, Poland (518-07-014-3171-03/18).
Corresponding Authors:  Correspondence Marcelina Krupa-Ma?kiewicz, E-mail:; Ireneusz Ochmian, E-mail:   

Cite this article: 

Marcelina Krupa-Ma?kiewicz, Jan Oszmiański, Sabina Lachowicz, Ma?gorzata Szczepanek, Bogus?awa Ja?kiewicz, Kamila Pachnowska, Ireneusz Ochmian. 2019.

Effect of nanosilver (nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions
. Journal of Integrative Agriculture, 18(8): 1871-1881.

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