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| Gene cloning and expression analyses of WBC genes in the developing grapevine seeds |
TANG Yu-jin1, WANG Qian1, XUE Jing-yi1, LI Yan1, 2, LI Rui-min1, Steve Van Nocker3, WANG Yue-jin1, ZHANG Chao-hong1 |
1 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture/State Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 College of Life Sciences, Northwest A&F University, Yangling 712100, P.R.China
3 Department of Horticulture, Michigan State University, East Lansing 48824, USA |
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摘要 :
Abstract White-brown complex (WBC) transporters, also called half-size ATP binding cassette G (ABCG) transporters, are involved in many biological processes, including seed development; however, the WBC transporters in grapevines received less attention to date. To reveal the molecular characteristics of WBCs and the connection between WBCs and agronomic traits of stenospermocarpic (seedless) grapevine, we carried out a genomic census and analysis of ovule-associated expression for VvWBC genes in grapevine. We identified 30 VvWBC genes and cloned full-length complementary DNAs (cDNAs) for 20 of these. The tissue or organ-specific expression analysis showed that several VvWBCs exhibited distinct expression patterns with some showing tissue specificity. Twelve VvWBC genes were found to be expressed in the developing ovules. Moreover, the results of quantitative real-time PCR (qRT-PCR) suggested that four of twelve ovule-expressed VvWBCs have distinct expression profiles during the development of ovules between seeded and stenospermocarpic grapevines. These four genes might be involved in ovule abortion. Meanwhile, chromosome mapping, multiple sequence alignments, exon/intron structure analyses and synteny analyses were preformed on VvWBC genes. Our experiments provide a new perspective on the mechanism of stenospermocarpic seedlessness and put forward a framework for further study of WBC transporters.
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Received: 24 June 2017
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31372023), and the earmarked fund for China Agricultural Research System (CARS-30-yz-7). |
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Corresponding Authors:
Correspondence ZHANG Chao-hong, Tel/Fax: +86-29-87082522, E-mail: zhangchaohong@nwafu.edu.cn
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| About author: TANG Yu-jin, E-mail: 15249217822@163.com |
Cite this article:
TANG Yu-jin, WANG Qian, XUE Jing-yi, LI Yan, LI Rui-min, Steve Van Nocker, WANG Yue-jin, ZHANG Chao-hong.
2018.
Gene cloning and expression analyses of WBC genes in the developing grapevine seeds. Journal of Integrative Agriculture, 17(06): 1348-1359.
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