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Journal of Integrative Agriculture
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TaTB1 suppresses wheat spikelet formation by interacting with TaDUO1 to synergistically activate WFZP transcription 

Baolian Lv1, 2, Hongyong Dai1, Tianqi Liu1, Fei Du1, Zhencheng Xie1, Xiuying Kong1, Chuan Xia1, Jijun Yan3, Jinfang Chu3, Jiaqiang Sun1, Xu Liu1#, Zhiyong Ni2#, Lichao Zhang1#

1 State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

2 Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms, College of Life Sciences, Xinjiang Agricultural University, Ürümqi 830052, China

3 Key Laboratory of Seed Innovation, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Highlights

l Genome editing of TaTB1 produces a multi-row spikelet phenotype with increased grain number.

l TaTB1 physically interacts with TaDUO1 to synergistically activate WFZP transcription.

TaTB1 modulates spike development in association with auxin level.

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摘要  

小麦穗部结构直接影响穗粒数,但其遗传调控机制仍不明确。本研究利用基因编辑技术,创制了小麦TaTB1基因在A、B、D三个亚基因组上同时敲除的突变体,发现该突变体呈现多小穗表型,且单穗小穗数和籽粒数均显著增加。转录组分析和分子生物学实验表明,TaTB1可直接激活穗发育关键基因WFZP的转录。进一步研究发现,TaTB1能够与转录因子TaDUO1物理互作协同增强WFZP的转录表达,从而共同参与小穗发育调控。此外,TaTB1突变体幼穗中生长素含量明显降低,提示TaTB1可能参与穗发育过程中生长素水平的调节。综上,本研究揭示了TaTB1–TaDUO1–WFZP分子模块在小麦小穗发育中的关键调控功能,为解析小麦穗部发育的分子网络及穗部结构遗传改良提供了重要的基因资源和理论依据。



Online: 14 September 2026  
Fund: 

This work was supported by the Agriculture Science and Technology Major Project, the Key Research and Development Program of Shandong Province (2024LZGCQY001), Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-CSCB-202401, CAAS-BRC-202609 CAAS-ZDRW202403, CAAS-CSAL-202401, and CAAS-BRC-CS-2025-01).

About author:  #Correspondence: Lichao Zhang, E-mail: zhanglichao@caas.cn; Zhiyong Ni, E-mail: nzy@xjau.edu.cn; Xu Liu, E-mail: liuxu03@caas.cn

Cite this article: 

Baolian Lv, Hongyong Dai, Tianqi Liu, Fei Du, Zhencheng Xie, Xiuying Kong, Chuan Xia, Jijun Yan, Jinfang Chu, Jiaqiang Sun, Xu Liu, Zhiyong Ni, Lichao Zhang. 2026.

TaTB1 suppresses wheat spikelet formation by interacting with TaDUO1 to synergistically activate WFZP transcription  . Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.09.024

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