Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
A weak OsBRI1 allele in Zhonghua 11 as a genetic tool for brassinosteroid signaling research in rice

Yanzhao Feng1*, Qingfeng Zhu1*, Qiuyue Yuan1, 2, Pei Chen1, Xielian Tan1, 2, Ning Huang1, 2, Jiao Xue1#, Yang Yu1#

1 Guangdong Key Laboratory of Crop Germplasm Resources Preservation and Utilization/Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

2 College of Agriculture, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

 Highlights 

Ÿ Zhonghua 11 and Taichung 65 exhibit distinct physiological and molecular responses to brassinosteroids despite their close japonica relationship.

Ÿ A near-isogenic d61-zh11 line was developed to transfer the classical BR-insensitive mutation into a modern functional genomics background.

Ÿ d61-zh11 provides a standardized reference mutant for reproducible and systems-level dissection of BR signaling in rice.

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

油菜素内酯(brassinosteroidsBRs)是调控水稻株型建成、发育进程和产量形成的重要植物激素。尽管同属粳稻亚种,中花11Zhonghua 11ZH11)与台中65Taichung 65TC65)在BR相关性状上仍存在明显差异,这与其遗传背景及育种历程的分化密切相关。由于株型紧凑、抽穗期短、遗传转化效率高,并拥有完整的T2T基因组序列,ZH11已成为当前水稻功能基因组研究的重要模式材料。然而,ZH11中缺乏诸如OsBRI1等关键BR信号通路突变体,在一定程度上制约了BR相关研究的开展。本研究从形态、生理和分子层面系统比较了ZH11TC65对外源BR处理的响应差异,揭示了两种材料在BR敏感性上的显著分化。基于此,我们通过连续回交并结合分子标记辅助选择,将TC65背景的弱等位d61-1突变导入ZH11背景,成功构建了近等基因系d61-zh11。该材料表现出适度的BR不敏感性和紧凑株型,遗传稳定,同时避免了强突变体常见的严重多效性缺陷。作为一种非转基因突变体,d61-zh11为在现代遗传背景下精细解析BR信号通路提供了理想平台,也可作为正向和反向遗传筛选的重要研究资源。本研究实现了经典激素遗传学与现代功能基因组学的有效衔接,为水稻BR相关基础研究和育种应用提供了重要的遗传材料。



Online: 05 February 2026  
Fund: 

This research was supported by the Special Funding for the Construction of the High-Level Academy of Agricultural Sciences (NYQS202606), the National Natural Science Foundation of China (32372066 and 32300454), the grants from Guangdong Province, China (2023A1515012075 and 2024A1515013064), the Guangzhou Science and Technology Programme (2024B03J1363), and the funding from the Guangdong Academy of Agricultural Sciences (R2021YJ-QG005, R2021YJ-YB3011 and R2022PY-QY011).

About author:  #Correspondence Jiao Xue, E-mail: xuejiao@gdaas.cn; Yang Yu, E-mail: yuyang@gdaas.cn * These authors contributed equally to the study.

Cite this article: 

Yanzhao Feng, Qingfeng Zhu, Qiuyue Yuan, Pei Chen, Xielian Tan, Ning Huang, Jiao Xue, Yang Yu. 2026. A weak OsBRI1 allele in Zhonghua 11 as a genetic tool for brassinosteroid signaling research in rice. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.02.008

Hou L, Zhu L, Hao M, Liang Y, Xiao G. 2025. Brassinosteroids enhance gibberellic acid biosynthesis to promote cotton fibre cell elongation. Plant Biotechnology Journal, 23, 1213-1215.

Joo S H, Jang M S, Kim M K, Lee J E, Kim S K, 2015. Biosynthetic relationship between C28-brassinosteroids and C29-brassinosteroids in rice (Oryza sativa) seedlings. Phytochemistry, 111, 84-90.

Li X, Li J, Zabed H M, Li J, Xiong M, Shi H, Li J. 2025. Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants. aBIOTECH6, 328-345.

Manghwar H, Hussain A, Ali Q, Liu F. 2022. Brassinosteroids (BRs) role in plant development and coping with different stresses. International Journal of Molecular Sciences23,1012.

Nakamura A, Fujioka S, Sunohara H, Kamiya N, Hong Z, Inukai Y, Miura K, Takatsuto S, Yoshida S, Ueguchi-Tanaka M, Hasegawa Y, Kitano H, Matsuoka M. 2006. The role of OsBRI1 and its homologous genes, OsBRL1 and OsBRL3, in rice. Plant Physiology140, 580-590.

Nam K H, Li J. 2002. BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell110, 203-212.

Yamamuro C, Ihara Y, Wu X, Noguchi T, Fujioka S, Takatsuto S, Ashikari M, Kitano H, Matsuoka M. 2000. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. The Plant Cell12, 1591-1605.

Yu Y, He R R, Yang L, Feng Y Z, Xue J, Liu Q, Zhou Y F, Lei M Q, Zhang Y C, Lian J P, Chen Y Q. 2024. A transthyretin-like protein acts downstream of miR397 and LACCASE to regulate grain yield in rice. The Plant Cell36, 2893-2907.

Yuan H, Xu Z, Chen W, Deng C, Liu Y, Yuan M, Gao P, Shi H, Tu B, Li T, Kang L, Ma B, Wang Y, Wang J, Chen X, Li S, Qin P. 2022. OsBSK2, a putative brassinosteroid-signalling kinase, positively controls grain size in rice. Journal of Experimental Botany73, 5529-5542.

Zhang C, Bai M Y, Chong K. 2014. Brassinosteroid-mediated regulation of agronomic traits in rice. Plant Cell Reports33, 683-696.

No related articles found!
No Suggested Reading articles found!