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Journal of Integrative Agriculture
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Risk assessment and residue behavior of a novel pesticide cyetpyrafen and its metabolites from fresh tea leaves to tea infusion

Yue Hu1, 2, Yating Ning1, 2, 3, Yan Zhao1, 4, Yaqi Wang1, 2, Fengjian Luo1, 2, 5, Li Zhou1, 5, Xinzhong Zhang1, 3, 5#

1 Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

3 Hangzhou Hope Agricultural Technology Co., Ltd, Hangzhou 310008, China

4 College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

5 National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Hangzhou 310008, China

 Highlights 

1. Residual dissipation of cyetpyrafen and its three metabolites was studied across tea cultivation, processing, and brewing.

2. Cyetpyrafen dissipated rapidly (t1/2=0.59 d) in the field, with M-309 identified as the major metabolite.

3. The dietary intake risk of cyetpyrafen from tea consumption was negligible to human health.

4. Cyetpyrafen and its main metabolites were predicted to be harmful to non-target organisms.

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摘要  

目前,茶园防治螨类害虫时,杀螨剂的选择范围非常有限。乙唑螨腈是一种高效、低毒和负温度系数的新型国产杀螨剂,为茶叶螨类害虫防治提供了一种潜在替代方案;然而,它在茶叶中的残留行为尚不清楚。本研究开发了一种同时检测不同茶基质中乙唑螨腈及其代谢物(M-309M-325-1M-409-3)残留的分析方法,并用于研究它们从茶园到茶杯中的残留降解行为。方法中目标化合物的平均回收率为73.4%~106.2%,相对标准偏差(RSD)低于12.0%;在茶叶种植过程中,乙唑螨腈的消解半衰期为0.59天,主要代谢产物为M-309。乙唑螨腈和代谢物M-309的残留量受茶叶不同加工阶段的影响,特别是受杀青、干燥和萎凋过程中的失水和高温影响。茶园喷施乙唑螨腈后的157天分别采样茶鲜叶加工成绿茶和红茶,加工过程中乙唑螨腈的总加工系数分别为1.39~1.71(绿茶)和1.48~2.28(红茶)。将绿茶和红茶冲泡茶汤时,乙唑螨腈的浸出率分别为7.4%6%。膳食风险评估结果表明,饮茶中摄入乙唑螨腈的风险较低,风险熵低于100%。然而,理论计算表明,乙唑螨腈代谢物对非目标生物存在潜在危害,需要值得注意。本研究结果为在茶园中安全合理施用乙唑螨腈提供了参考。



Abstract  

China has limited acaricide options for tea plantations. Cyetpyrafen, a novel domestic acaricide with high efficacy, low toxicity and a negative temperature coefficient, offers an alternative for tea pest control; however, its residue fate in tea remains unclear. This study developed a method to simultaneously detect cyetpyrafen and its metabolites (M-309, M-325-1, and M-409-3) in different tea matrices to investigate their fate. Recoveries of compounds ranged from 73.4% to 106.2% with the relative standard deviations (RSDs) below 12.0%. During tea cultivation, the dissipation half-life of cyetpyrafen was 0.59 d, with M-309 as a major metabolite. The residues of cyetpyrafen and M-309 were affected by different processing stages, especially water loss and high temperatures during fixing, drying and withering. The total processing factors ranged from 1.39 to 1.71 for green tea and 1.48 to 2.28 for black tea (processed from fresh tea leaves sampled at 1, 5, and 7 d), respectively. The leaching rates of cyetpyrafen from green tea and black tea into tea infusions were 7.4% and 6%, respectively. The risk associated with cyetpyrafen intake from tea consumption was low, with risk quotient values below 100%. However, theoretical calculation indicated potential harm to non-target organisms from its metabolites. This research provides a reference for the safe and efficient use of cyetpyrafen in tea gardens.

Keywords:  cyetpyrafen       leaching rate       tea       residue dissipation       processing  
Online: 07 January 2026  
Fund: 

This work was financially supported by the National Key Research and Development Program of China (2022YFD1600803), the National Natural Science Foundation of China (31772077), the Yunnan Provincial Tea Industry Technology Innovation Center (202505AK340010) and the Chinese Academy of Agricultural Sciences for Agricultural Science and Technology Innovation Program of Tea Research Institute (CAAS-ASTIP-2021-TRI).

About author:  #Correspondence Xinzhong Zhang, E-mail: zxz.1982@163.com, zhangxinzhong@tricaas.com

Cite this article: 

Yue Hu, Yating Ning, Yan Zhao, Yaqi Wang, Fengjian Luo , Li Zhou, Xinzhong Zhang. 2026. Risk assessment and residue behavior of a novel pesticide cyetpyrafen and its metabolites from fresh tea leaves to tea infusion. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.01.001

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