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Journal of Integrative Agriculture
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Genetic diversity of H13 avian influenza viruses in migratory shorebirds in eastern China

Mengdi Guan1*, Wenxi Li1*, Wenjun Shi2*, Mengjing Wang1, Lili Liu1, Yujiao Xie1, Huihui Kong3, Yujie Li4, Hongke Xu1, Mingfeng Liu1, Xuan Yang1, Zhanshuai Li1, Anran Ren1, Jing Guo1#, Xuyong Li1#

1 College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China

Technology Center of Qindao Customs, Qingdao 266000, China

2 Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory for Animal Disease Control and Prevention, Harbin 150069, China

3 Shandong Provincial Center for Animal Disease Control, Jinan 250100, China.

 Highlights: 

1. Twenty one H13 subtype avian influenza viruses were isolated from 50016 samples in migratory birds during 2017 to 2024 in Shandong Province, China.

2. The H13 viruses were mainly harbored by shorebirds (gulls) and exhibited considerable genetic diversity.

3. Extensive gene segment reassortment between H13/H16 like viruses and other subtypes were observed.

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摘要  

H13亚型禽流感病毒的主要宿主是水鸟家禽和哺乳动物中鲜有发现,目前对其流行病学动态及遗传特征的认知仍较为欠缺。为填补这一研究空白,本研究于2017-2024年在我国山东省主要候鸟栖息地开展了系统性的主动监测累计采集50016份候鸟样本,共分离到21H13亚型禽流感病毒(19H13N62H13N8。所有H13亚型病毒均发现于2021-2024年的春秋迁徙季,且绝大多数源自鸥类。贝叶斯系统发育分析显示,21病毒HA基因分属欧亚谱系和北美-欧亚谱系,而N6N8基因则均聚类于欧亚谱系。本研究还发现H13/H16-like病毒存在广泛的基因重配现象,如其内部基因片段参与高致病性H5N1毒株的形成,部分毒株的基因与感染人类的H10N5亚型病毒基因具有高度同源性。此外,H13病毒在小鼠体内复制能力较差,表明其公共卫生风险较低。本研究深化了对H13亚型禽流感病毒全球流行特征、地理分布、宿主范围及系统发育多样性的认知,同时也凸显了针对迁徙鸟类开展持续性监测的必要性。



Online: 22 December 2025  
Fund: 

This work was supported by the National Natural Science Foundation of China (32202798, 32570621) and the “Unveiling and Commanding” of General Administration of Customs of China (2024HK306).

About author:  #Correspondence Xuyong Li, E-mail: lixuyong@lcu.edu.cn; Jing Guo, E-mail: guojing@lcu.edu.cn * These authors contributed equally to this work.

Cite this article: 

Mengdi Guan, Wenxi Li, Wenjun Shi, Mengjing Wang, Lili Liu, Yujiao Xie, Huihui Kong, Yujie Li, Hongke Xu, Mingfeng Liu, Xuan Yang, Zhanshuai Li, Anran Ren, Jing Guo, Xuyong Li. 2025. Genetic diversity of H13 avian influenza viruses in migratory shorebirds in eastern China. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.12.043

Hill N J, Bishop M A, Trovão N S, Ineson K M, Schaefer A L, Puryear W B, Zhou K, Foss A D, Clark D E, MacKenzie K G, Gass J D, Jr., Borkenhagen L K, Hall J S, Runstadler J A. 2022. Ecological divergence of wild birds drives avian influenza spillover and global spread. PLoS Pathogens, 18, e1010062

Karakus U, Mena I, Kottur J, El Zahed S S, Seoane R, Yildiz S, Chen L, Plancarte M, Lindsay L, Halpin R, Stockwell T B, Wentworth D E, Boons G J, Krammer F, Stertz S, Boyce W, de Vries R P, Aggarwal A K, García-Sastre A. 2024. H19 influenza A virus exhibits species-specific MHC class II receptor usage. Cell Host and Microbe, 32, 1089-1102.e1010

Lan J, Liu L, Li X, Ding R, Zhu J, Qu F, Zhai B, Wu J, Wang Y, Yang S, Chai H, Zeng X. 2025. H13N8 and H16N3 AIV Isolated From Shorebirds Can Replicate in the Rectum of Mice. Journal of Medical Virology, 97, e70455

Li X, Li A, Qu F, Li Y, Chen F, Lv X, An Q, Fei M, Chen H, Liang H, Zhang X, Li J, Yu M, Qin S, Xie L, Yin S, Huang Z, Yang S, Sun H, Li X, Chai H. 2025. Evaluation of global distribution, genetic evolution, and mammalian infectivity and pathogenicity of H13 and H16 avian influenza viruses. Emerging Microbes & Infections, 14, 2482695

Olsen B, Munster V J, Wallensten A, Waldenström J, Osterhaus A D, Fouchier R A. 2006. Global patterns of influenza a virus in wild birds. Science, 312, 384-388

Peng P, Shen J, Shi W, Guo J, Wang M, Li W, Yue Z, Sun X, Guan M, Liu L, Xu H, Xie Y, Ren A, Liu M, Liu W, Zhang Z, Xiao Z, Li X. 2024. Novel H16N3 avian influenza viruses isolated from migratory gulls in China in 2023. Frontiers in Microbiology, 15, 1543338

Shi J, Zeng X, Cui P, Yan C, Chen H. 2023. Alarming situation of emerging H5 and H7 avian influenza and effective control strategies. Emerging Microbes & Infections, 12, 2155072

Sun W, Zhao M, Yu Z, Li Y, Zhang X, Feng N, Wang T, Wang H, He H, Zhao Y, Yang S, Xia X, Gao Y. 2023. Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals. Emerging Microbes & Infections, 12, e2184177

Verhagen J H, Majoor F, Lexmond P, Vuong O, Kasemir G, Lutterop D, Osterhaus A D, Fouchier R A, Kuiken T. 2014. Epidemiology of influenza A virus among black-headed gulls, the Netherlands, 2006-2010. Emerging Infectious Diseases, 20, 138-141

Verhagen J H, Poen M, Stallknecht D E, van der Vliet S, Lexmond P, Sreevatsan S, Poulson R L, Fouchier R A M, Lebarbenchon C. 2020. Phylogeography and Antigenic Diversity of Low-Pathogenic Avian Influenza H13 and H16 Viruses. Journal of Virology, 94 

Webster R G, Govorkova E A. 2014. Continuing challenges in influenza. Annals of the New York Academy of Sciences, 1323, 115-139

Yang J, Zheng S, Sun J, Wu H, Zhang D, Wang Y, Tian T, Zhu L, Wu Z, Li L, Gao G F, Bi Y, Yao H. 2025. A human-infecting H10N5 avian influenza virus: Clinical features, virus reassortment, receptor-binding affinity, and possible transmission routes. Journal of Infection, 90, 106456

Zeng X, Shi J, Chen H. 2024. Control of highly pathogenic avian influenza through vaccination. Journal of Integrative Agriculture, 23, 1447-1453

Zhang H, Guo J, Peng P, Wang M, Shen J, Sun X, Guan M, Cui P, Deng G, Chu D, Li X. 2025. Evolution and biological characteristics of the circulated H8N4 avian influenza viruses. Journal of Integrative Agriculture, 24, 2342-2355 

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