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Journal of Integrative Agriculture
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Coronavirus-like particles package the negative-strand genome of coronavirus

Xinyu Yang*, Wenzhen Qin*, Ning Kong*, Yuchang Liu, Guangzhi Tong, Tongling Shan#

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China

 Highlights 

1. The particles of coronavirus package the negative-sense genome of coronavirus into the coronavirus-like particles.

2. In PEDV particles purified by sucrose density gradient centrifugation and treated with antibodies and nuclease, approximately 20% to 46% contained negative-sense genomes.

3. Coronavirus-like particles are capable of adsorption and internalization.

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摘要  

冠状病毒是单链正义 RNA 病毒。在 PEDV 吸附和内化后及病毒负链 RNA 合成前,我们同时检测到了冠状病毒的正链和负链序列。为解释上述发现,本研究证实α、β和δ冠状病毒属的冠状病毒病毒粒子中包裹病毒的负链基因组。通过蔗糖密度梯度离心纯化,并经抗体和核酸酶处理的 PEDV 颗粒中,约 20% 至 46% 含有负链基因组,且这些病毒颗粒具备吸附和内化的能力。该研究解释了冠状病毒在吸附和内化后检测到病毒的负链RNA的发现,也表明了冠状病毒在组装过程中不仅包装病毒正链基因组,也会同时包装了病毒负链基因组。



Online: 22 December 2025  
Fund: 

This work was supported by the National Key Research and Development Programs of China (2023YFD1801300) and the National Natural Science Foundation of China (32272999).

About author:  # Correspondence Tongling Shan, E-mail: shantongling@shvri.ac.cn * These authors contributed equally to this work.

Cite this article: 

Xinyu Yang, Wenzhen Qin, Ning Kong, Yuchang Liu, Guangzhi Tong, Tongling Shan. 2025. Coronavirus-like particles package the negative-strand genome of coronavirus. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.12.042

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