Abramson J, Adler J, Dunger J, Evans R, Green T, Pritzel A, Ronneberger O, Willmore L, Ballard A J, Bambrick J, Bodenstein S W, Evans D A, Hung C C, O’Neill M, Reiman D, Tunyasuvunakool K, Wu Z, Žemgulytė A, Arvaniti E, Beattie C, et al. 2024. Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630, 493–500.

Agnew-Francis K A, Tang Y, Lin X, Low Y S, Wun S J, Kuo A, Elias S, Lonhienne T, Condon N D, Pimentel B, Vergani C E, Smith M T, Fraser J A, Williams C M, Guddat L W. 2020. Herbicides that target acetohydroxyacid synthase are potent inhibitors of the growth of drug-resistant Candida auris. ACS Infectious Diseases, 6, 2901–2912.

Bandbe C D, Patil K S, Pathan E K. 2024. Tuning fungal promoters for the expression of eukaryotic proteins. World Journal of Microbiology and Biotechnology, 40, 400.

Bhunjun C S, Phukhamsakda C, Jayawardena R S, Jeewon R, Promputtha I, Hyde K D. 2021. Investigating species boundaries in Colletotrichum. Fungal Diversity, 107, 107–127.

Chen X, Selvaraj P, Lin L, Fang W, Wu C, Yang P, Zhang J, Abubakar Y S, Yang F, Lu G, Liu W, Wang Z, Naqvi N I, Zheng W. 2023. Rab7/Retromer-based endolysosomal trafficking is essential for proper host invasion in rice blast. New Phytologist, 239, 1384–1403.

Cheng Y, Lonhienne T, Garcia M D, Williams C M, Schenk G, Guddat L W. 2023. Crystal structure of the commercial herbicide, amidosulfuron, in complex with Arabidopsis thaliana acetohydroxyacid synthase. Journal of Agricultural and Food Chemistry, 71, 5117–5126.

Cheng Y, Wang Y, Lonhienne T, Wang J G, Guddat L W. 2024. Crystal structures of Arabidopsis thaliana acetohydroxyacid synthase in complex with the herbicide triasulfuron and two analogues with herbicidal activity in field trials. Journal of Agricultural and Food Chemistry, 72, 22487–22498.

Chong N F, Van De Wouw A P, Idnurm A. 2024. The ilv2 gene, encoding acetolactate synthase for branched chain amino acid biosynthesis, is required for plant pathogenicity by Leptosphaeria maculans. Molecular Biology Reports, 51, 682.

D'Ávila L S, De Filippi M C C, Café-Filho A C. 2022. Fungicide resistance in Pyricularia oryzae populations from southern and northern Brazil and evidence of fitness costs for QoI-resistant isolates. Crop Protection, 153, 105887.

Damm U, O’Connell R J, Groenewald J Z, Crous P W. 2014. The Colletotrichum destructivum species complex-hemibiotrophic pathogens of forage and field crops. Studies in Mycology, 79, 49–84.

Dean R, Van Kan J A, Pretorius Z A, Hammond-Kosack K E, Di Pietro A, Spanu P D, Rudd J J, Dickman M, Kahmann R, Ellis J, Foster G D. 2012. The Top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13, 414–430.

Díaz-Holguín A, Saarinen M, Vo D D, Sturchio A, Branzell N, Cabeza De Vaca I, Hu H, Mitjavila-Domènech N, Lindqvist A, Baranczewski P, Millan M J, Yang Y, Carlsson J, Svenningsson P. 2024. AlphaFold accelerated discovery of psychotropic agonists targeting the trace amine-associated receptor 1. Science Advances, 10, eadn1524.

Du Y, Zhang H, Hong L, Wang J, Zheng X, Zhang Z. 2013. Acetolactate synthases MoIlv2 and MoIlv6 are required for infection-related morphogenesis in Magnaporthe oryzae. Molecular Plant Pathology, 14, 870–884.

Duan L, Wang L, Chen W, He Z, Zhou E, Zhu Y. 2023. Deficiency of ChPks and ChThr1 inhibited DHN-melanin biosynthesis, disrupted cell wall integrity and attenuated pathogenicity in Colletotrichum higginsianum. International Journal of Molecular Sciences, 24, 15890.

Eberhardt J, Santos-Martins D, Tillack A F, Forli S. 2021. AutoDock Vina 1.2.0: New docking methods, expanded force field, and python bindings. Journal of Chemical Information and Modeling, 61, 3891–3898.

Fisher M C, Gurr S J, Cuomo C A, Blehert D S, Jin H, Stukenbrock E H, Stajich J E, Kahmann R, Boone C, Denning D W, Gow N A R, Klein B S, Kronstad J W, Sheppard D C, Taylor J W, Wright G D, Heitman J, Casadevall A, Cowen L E. 2020. Threats posed by the fungal kingdom to humans, wildlife, and agriculture. mBio, 11, e00449–20.

Gai Y, Wang H. 2024. Plant disease: A growing threat to global food security. Agronomy, 14, 1615.

Garcia M D, Chua S M H, Low Y S, Lee Y T, Agnew-Francis K, Wang J G, Nouwens A, Lonhienne T, Williams C M, Fraser J A, Guddat L W. 2018. Commercial AHAS-inhibiting herbicides are promising drug leads for the treatment of human fungal pathogenic infections. Proceedings of the National Academy of Sciences of the United States of America, 115, E9649–E9658.

Gutiérrez-Alonso O, Hawkins N J, Cools H J, Shaw M W, Fraaije B A. 2017. Dose-dependent selection drives lineage replacement during the experimental evolution of SDHI fungicide resistance in Zymoseptoria tritici. Evolutionary Applications, 10, 1055–1066.

Homma F, Huang J, Van Der Hoorn R A L. 2023. AlphaFold-Multimer predicts cross-kingdom interactions at the plant–pathogen interface. Nature Communications, 14, 6040.

Jeon J, Park S Y, Chi M H, Choi J, Park J, Rho H S, Kim S, Goh J, Yoo S, Choi J, Park J Y, Yi M, Yang S, Kwon M J, Han S S, Kim B R, Khang C H, Park B, Lim S E, Jung K, et al. 2007. Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature Genetics, 39, 561–565.

Jubb H C, Higueruelo A P, Ochoa-Montaño B, Pitt W R, Ascher D B, Blundell T L. 2017. Arpeggio: A web server for calculating and visualising interatomic interactions in protein structures. Journal of Molecular Biology, 429, 365–371.

Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, Tunyasuvunakool K, Bates R, Žídek A, Potapenko A, Bridgland A, Meyer C, Kohl S A A, Ballard A J, Cowie A, Romera-Paredes B, Nikolov S, Jain R, Adler J, Back T, et al. 2021. Highly accurate protein structure prediction with AlphaFold. Nature, 596, 583–589.

Koreki A, Michel S, Lebeaux C, Trouilh L, Délye C. 2024. Prevalence, spatial structure and evolution of resistance to acetolactate-synthase (ALS) inhibitors and 2,4-D in the major weed Papaver rhoeas (L.) assessed using a massive, country-wide sampling. Pest Management Science, 80, 637–647.

Kück U, Hoff B. 2005. Application of the nourseothricin acetyltransferase gene (nat1) as dominant marker for transformation of filamentous fungi. Fungal Genetics Reports, 53, 9–11.

Liu H, Lee G, Sang H. 2024. Exploring SDHI fungicide resistance in Botrytis cinerea through genetic transformation system and AlphaFold model-based molecular docking. Pest Management Science, 80, 5954–5964.

Lonhienne T, Low Y S, Garcia M D, Croll T, Gao Y, Wang Q, Brillault L, Williams C M, Fraser J A, Mcgeary R P, West N P, Landsberg M J, Rao Z, Schenk G, Guddat L W. 2020. Structures of fungal and plant acetohydroxyacid synthases. Nature, 586, 317–321.

Lv W, Xu Z, Talbot N J, Wang Z. 2020. The sorting nexin FgAtg20 is involved in the Cvt pathway, non-selective macroautophagy, pexophagy and pathogenesis in Fusarium graminearum. Cellular Microbiology, 22, e13208.

Mahmoudjanlou Y, Hoff B, Kück U. 2019. Construction of a codon-adapted nourseotricin-resistance marker gene for efficient targeted gene deletion in the mycophenolic acid producer Penicillium brevicompactum. Journal of Fungi, 5, 96.

McCourt J A, Duggleby R G. 2006. Acetohydroxyacid synthase and its role in the biosynthetic pathway for branched-chain amino acids. Amino Acids, 31, 173–210.

Meyer V, Andersen M R, Brakhage A A, Braus G H, Caddick M X, Cairns T C, De Vries R P, Haarmann T, Hansen K, Hertz-Fowler C, Krappmann S, Mortensen U H, Peñalva M A, Ram A F J, Head R M. 2016. Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: A white paper. Fungal Biology and Biotechnology, 3, 6.

Morris G M, Huey R, Lindstrom W, Sanner M F, Belew R K, Goodsell D S, Olson A J. 2009. AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. Journal of Computational Chemistry, 30, 2785–2791.

O’Boyle N M, Banck M, James C A, Morley C, Vandermeersch T, Hutchison G R. 2011. Open Babel: An open chemical toolbox. Journal of Cheminformatics, 3, 33.

O’Connell R, Herbert C, Sreenivasaprasad S, Khatib M, Esquerré-Tugayé M T, Dumas B. 2004. A novel Arabidopsis-Colletotrichum pathosystem for the molecular dissection of plant-fungal interactions. Molecular Plant-Microbe Interactions, 17, 272–282.

O’Connell R J, Thon M R, Hacquard S, Amyotte S G, Kleemann J, Torres M F, Damm U, Buiate E A, Epstein L, Alkan N, Altmüller J, Alvarado-Balderrama L, Bauser C A, Becker C, Birren B W, Chen Z, Choi J, Crouch J A, Duvick J P, Farman M A, et al. 2012. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nature Genetics, 44, 1060–1065.

Pang S S, Duggleby R G, Schowen R L, Guddat L W. 2004. The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate. Journal of Biological Chemistry, 279, 2242–2253.

Pang S S, Guddat L W, Duggleby R G. 2002. Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. Journal of Biological Chemistry, 278, 7639–7644.

Patzoldt W L, Tranel P J. 2007. Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Science, 55, 421–428.

Peri K V R, Faria-Oliveira F, Larsson A, Plovie A, Papon N, Geijer C. 2023. Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia. FEMS Yeast Research, 23, foad016.

Rutter B D, Chu T T, Dallery J F, Zajt K K, O’Connell R J, Innes R W. 2022. The development of extracellular vesicle markers for the fungal phytopathogen Colletotrichum higginsianum. Journal of Extracellular Vesicles, 11, e12216.

Sang H, Hulvey J P, Green R, Xu H, Im J, Chang T, Jung G. 2018. A xenobiotic detoxification pathway through transcriptional regulation in filamentous fungi. mBio, 9, e00457–18.

Sfeir A, Symington L S. 2015. Microhomology-mediated end joining: A back-up survival mechanism or dedicated pathway? Trends in Biochemical Sciences, 40, 701–714.

Shen Q, Liang M, Yang F, Deng Y Z, Naqvi N I. 2020. Ferroptosis contributes to developmental cell death in rice blast. New Phytologist, 227, 1831–1846.

Sherriff C, Whelan M J, Arnold G M, Lafay J F, Brygoo Y, Bailey J A. 1994. Ribosomal DNA sequence analysis reveals new species groupings in the genus Colletotrichum. Experimental Mycology, 18, 121–138.

Sidhu Y S, Cairns T C, Chaudhari Y K, Usher J, Talbot N J, Studholme D J, Csukai M, Haynes K. 2015. Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. Fungal Genetics and Biology, 79, 102–109.

Stecher G, Tamura K, Kumar S. 2020. Molecular evolutionary genetics analysis (MEGA) for macOS. Molecular Biology and Evolution, 37, 1237–1239.

Steinkellner G, Kroutil W, Gruber K, Gruber C C. 2025. AlphaFold 3 is great-but it still needs human help to get chemistry right. Nature, 637, 548.

Takahara H, Hacquard S, Kombrink A, Hughes H B, Halder V, Robin G P, Hiruma K, Neumann U, Shinya T, Kombrink E, Shibuya N, Thomma B P, O’Connell R J. 2016. Colletotrichum higginsianum extracellular LysM proteins play dual roles in appressorial function and suppression of chitin-triggered plant immunity. New Phytologist, 211, 1323–1337.

Tunyasuvunakool K, Adler J, Wu Z, Green T, Zielinski M, Žídek A, Bridgland A, Cowie A, Meyer C, Laydon A, Velankar S, Kleywegt G J, Bateman A, Evans R, Pritzel A, Figurnov M, Ronneberger O, Bates R, Kohl S A A, Potapenko A, et al. 2021. Highly accurate protein structure prediction for the human proteome. Nature, 596, 590–596.

Wei C, Qin T, Li Y, Wang W, Dong T, Wang Q. 2020. Host-induced gene silencing of the acetolactate synthases VdILV2 and VdILV6 confers resistance to Verticillium wilt in cotton (Gossypium hirsutum L.). Biochemical and Biophysical Research Communications, 524, 392–397.

Xia Y, Sun G, Xiao J, He X, Jiang H, Zhang Z, Zhang Q, Li K, Zhang S, Shi X, Wang Z, Liu L, Zhao Y, Yang Y, Duan K, Ye W, Wang Y, Dong S, Wang Y, Ma Z, et al. 2024. AlphaFold-guided redesign of a plant pectin methylesterase inhibitor for broad-spectrum disease resistance. Molecular Plant, 17, 1344–1368.

Xie L, Zang X, Cheng W, Zhang Z, Zhou J, Chen M, Tang Y. 2021. Harzianic acid from Trichoderma afroharzianum is a natural product inhibitor of acetohydroxyacid synthase. Journal of the American Chemical Society, 143, 9575–9584.

Xu Q, Gao Y, Sun Z, Shi J R, Tang J Y, Wang Y, Liu Y, Sun X W, Li H R, Lonhienne T G, Niu C W, Li Y H, Guddat L W, Wang J G. 2024. Chemical synthesis, herbicidal activity, crop safety, and molecular basis of ortho-fluoroalkoxy substituted sulfonylureas as novel acetohydroxyacid synthase inhibitors. Journal of Agricultural and Food Chemistry, 72, 22595–22605.

Yamada T, Makimura K, Hisajima T, Ito M, Umeda Y, Abe S. 2008. Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker. Journal of Dermatological Science, 49, 53–61.

Yan Y, Yuan Q, Tang J, Huang J, Hsiang T, Wei Y, Zheng L. 2018. Colletotrichum higginsianum as a model for understanding host-pathogen interactions: A review. International Journal of Molecular Sciences, 19, 2142.

Yang F, Naqvi N I. 2014. Sulfonylurea resistance reconstitution as a novel strategy for ILV2-specific integration in Magnaporthe oryzae. Fungal Genetics and Biology, 68, 71–76.

Yonehara K, Kumakura N, Motoyama T, Ishihama N, Dallery J F, O’Connell R, Shirasu K. 2023. Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3-based marker recycling. Molecular Plant Pathology, 24, 1451–1464.

Zhou Q, Liu W, Zhang Y, Liu K K. 2007. Action mechanisms of acetolactate synthase-inhibiting herbicides. Pesticide Biochemistry and Physiology, 89, 89–96.

Zhu Y, Duan L, Zhu C, Wang L, He Z, Yang M, Zhou E. 2023. Dual transcriptome analysis reveals that ChATG8 is required for fungal development, melanization and pathogenicity during the interaction between Colletotrichum higginsianum and Arabidopsis thaliana. International Journal of Molecular Sciences, 24, 4376.