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Journal of Integrative Agriculture
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Point-of-Care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips

Zezhao Cao1*, Junchao Shi1*, Ruijie Hu1, Jun Xue1, Gaili Wang2, Zi Li1, Huabo Yu1, Wei Liu1, Wenqi He1, Hualei Wang1, Haili Zhang1#, Yungang Lan1#

1 State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China

2Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China

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摘要  

猪德尔塔冠状病毒(PDCoV感染引起仔猪严重腹泻、脱水甚至死亡,威胁养猪业发展,且PDCoV具备跨种传播的潜能,存在不容忽视的人兽共患病风险。因此,开发PDCoV现场快速检测工具对防控至关重要。本研究针对 PDCoV 高度保守的核衣壳(N)蛋白,建立胶体金和荧光微球两种免疫层析试纸条。首先,原核表达系统表达并纯化获得N蛋白免疫小鼠采用杂交瘤技术筛选出 4 株高亲和力单克隆抗体。经配对优化,选择最优组合制备胶体金与荧光微球标记试纸,均可在 5 分钟内完成检测。胶体金试纸条对病毒感染细胞培养物的检测下限为 102.3 TCID50/0.1 mL,荧光试纸条灵敏度更高,达101.7 TCID50/0.1 mL。两种试纸条均高度特异,与猪血凝性脑脊髓炎病毒(PHEV)、伪狂犬病病毒(PRV)、猪圆环病毒 2 型(PCV2)、猪圆环病毒 3 型(PCV3塞内卡病毒(SVA牛疱疹病毒4型(BoHV-4均无交叉反应。两种试纸条在室温下可稳定保存 6 个月,有较好的稳定性。临床测试腹泻仔猪样本显示:胶体金试纸条与 qRT-PCR 符合率 90.32%28/31),荧光试纸条与 qRT-PCR 符合率为 96.77%30/31)。结果表明,本研究建立的两种试纸条操作简便、快速,无需专业设备肉眼或便携紫外灯即可判读结果具有良好临床应用潜力,为 PDCoV 现场筛查、疫情溯源及跨物种传播监测提供了重要技术支持。



Online: 05 August 2025  
Fund: 

This work was financially supported by the National Key Research and Development Program of China (grant No.2021YFF0703600).

About author:  Zezhao Cao, E-mail: 599142457@qq.com; Junchao Shi, E-mail: sjc@jlu.edu.cn; #Correspondence Haili Zhang, E-mail: zhanghaili@jlu.edu.cn; Yungang Lan, lanyungang@jlu.edu.cn * These authors contributed equally to this work.

Cite this article: 

Zezhao Cao, Junchao Shi, Ruijie Hu, Jun Xue, Gaili Wang, Zi Li, Huabo Yu, Wei Liu, Wenqi He, Hualei Wang, Haili Zhang, Yungang Lan. 2025. Point-of-Care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.08.001

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