Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene

Tao Zhang, Jiaqi Tang, Qianfeng Li, Lin Zhang, Changquan Zhang, Qiaoquan Liu#, Dongsheng Zhao#

Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, College of Agriculture, Yangzhou University, Yangzhou 225009, China

 Highlights: 

(1) Most japonica rice varieties possess similar alleles for grain size, producing short and wide grains.

(2) The gs9ko allele has widely applicable effect for grain appearance improvement.

(3) The increased leaf angle of gs9ko allele does not affect it improvement effect. 

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

粒形是决定水稻产量和品质的重要因素。功能缺失型gs9等位基因可以产生细长粒形和较低垩白,影响籽粒外观。本研究中我们证实了当前大多数的粳稻品种表现为短圆粒,其主要粒形基因位点上携带相同的等位基因组合。利用CRISPR/Cas9产生的敲除等位基因gs9KO能够显著改善供试粳稻品种的粒形和垩白,且对产量性状无影响。此外,通过栽培密度试验确认gs9KO等位基因造成的植株叶夹角略有增加,不影响最终单株产量。结果表明,gs9KO等位基因在改善籽粒外观品质方面具有广泛的应用潜力。



Online: 03 April 2025  
Fund: 

This work was supported by grants from the Government of Jiangsu Province, China (BE2023331, JBGS[2021]001, 23KJA210001, BE2021334, BM2022008-02, KYCX23_3575) and the National Natural Science Foundation of China (31971914).

About author:  #Correspondence Dongsheng Zhao, E-mail: dszhao@yzu.edu.cn; Qiaoquan Liu, E-mail: qqliu@yzu.edu.cn

Cite this article: 

Tao Zhang, Jiaqi Tang, Qianfeng Li, Lin Zhang, Changquan Zhang, Qiaoquan Liu, Dongsheng Zhao. 2025. Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.04.002

Chen L, Li X, Zheng M, Hu R, Dong J, Zhou L, Liu W, Liu D, Yang W. 2024. Genes controlling grain chalkiness in rice. The Crop Journal, 12, 979-991.

Huang H, Qian Q. 2017. Progress in genetic research of rice grain shape and breeding achievements of long-grain shape and good quality japonica rice. Chinese Journal of Rice Science, 31, 665-672.

Jiang L, Wu L, Wang Y, Xu Q, Xu Z. Chen W. 2022. Research progress on the divergence and genetic basis of agronomic traits in xian and geng rice. The Crop Journal, 10, 924-931.

Lin S, Liu Z, Zhang K, Yang W, Zhan P, Tan Q, Gou Y, Ma S, Luan X, Huang C, Xiao Z, Liu Y, Zhu B, Liang R, Zhou W, Zhu H, Bu S, Liu G, Zhang G, Wang S. 2023. GL9 from Oryza glumaepatula controls grain size and chalkiness in rice. The Crop Journal, 11, 198-207.

Ren D, Ding C, Qian Q. 2023. Molecular bases of rice grain size and quality for optimized productivity. Science Bulletin, 68, 314-350.

Tong H, Chu C. 2018. Functional specificities of brassinosteroid and potential utilization for crop improvement. Trends in Plant Science, 23, 1016-1028.

Wang S, Li S, Liu Q, Wu K, Zhang J, Wang S, Wang Y, Chen X, Zhang Y, Gao C, Wang F, Huang H, Fu X. 2015. The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality. Nature Genetics, 47, 949-954.

Wang S, Wu K, Yuan Q, Liu X, Liu Z, Lin X, Zeng R, Zhu H, Dong G, Qian Q, Zhang G, Fu X. 2012. Control of grain size shape and quality by OsSPL16 in rice. Nature Genetics, 44, 950-954.

Wang Y, Xiong G, Hu J, Jiang L, Yu H, Xu J, Fang Y, Zeng L, Xu E, Xu J, Ye W, Meng X, Liu R, Chen H, Jing Y, Wang Y, Zhu X, Li J, Qian Q. 2015. Copy number variation at the GL7 locus contributes to grain size diversity in rice. Nature Genetics, 47, 944-952.

Wu B, Yun P, Zhou H, Xia D, Gu Y, Li P, Yao J, Zhou Z, Chen J, Liu R, Cheng S, Zhang H, Zheng Y, Lou G, Chen P, Wan S, Zhou M, Li Y, Gao G, Zhang Q, et al. 2022. Natural variation in WHITE-CORE RATE 1 regulates redox homeostasis in rice endosperm to affect grain quality. Plant Cell, 34, 1912-1932.

Yang W, Zhan P, Lin S, Gou Y, Zhang G, Wang S. 2019. Research progress of grain shape genetics in rice. Journal of South China Agricultural University, 40, 203-210.

Zhang T, Wang Z, Liu Q, Zhao D. 2025. Genetic improvement of rice grain size using the CRISPR/Cas9 system. Rice, 18, 3.

Zhang Y, Massel K, Godwin ID, Gao C. 2018. Applications and potential of genome editing in crop improvement. Genome Biology, 19, 210.

Zhao D, Li Q, Zhang C, Zhang C, Yang Q, Pan L, Ren X, Lu J, Gu M, Liu Q. 2018. GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality. Nature Communications, 9, 1240.

Zhao D, Liu J, Ding A, Zhang T, Ren X, Zhang L, Li Q, Fan X, Zhang C, Liu Q. 2021. Improving grain appearance of erect-panicle japonica rice cultivars by introgression of the null gs9 allele. Journal of Integrative Agriculture, 20, 2032-2042.

Zhao D, Zhang C, Li Q, Liu Q. 2022. Genetic control of grain appearance quality in rice. Biotechnology Advances, 60, 108014. 

No related articles found!
No Suggested Reading articles found!