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| Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol, Vitexin, and Isovitexin Contents in Mung Bean |
| YAO Yang, CHENG Xu-zhen , REN Gui-xing |
| 1.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.
Abstract Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.
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Received: 29 September 2010
Accepted:
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| Fund: The present study was supported by the Talent Fund (to Dr. Ren Guixing) from the Chinese Academy of Agricultural Sciences and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (nycytx-018). |
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Corresponding Authors:
Correspondence REN Gui-xing, Tel: +86-10-62115596, Fax: +86-10-62156596, E-mail: renguixing@caas.net.cn
E-mail: renguixing@caas.net.cn
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Cite this article:
YAO Yang, CHENG Xu-zhen , REN Gui-xing.
2011.
Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol, Vitexin, and Isovitexin Contents in Mung Bean. Journal of Integrative Agriculture, 10(12): 1986-1991.
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