Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat
Fei Wang1, 2*, Xin Zhao1, 2*, Xianghai Yu1, 2, Wei Zhu1, 2, Lili Xu2, Yiran Cheng1, Yazhou Zhang1, 2, Yi Wang1, 2, Jian Zeng3, Xing Fan1, 2, Lina Sha4, Haiqin Zhang4, Yonghong Zhou1, 2, Dandan Wu1, 2#, Houyang Kang1, 2#

1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China 

2 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China

3 College of Resources, Sichuan Agricultural University, Chengdu 611130, China

4 College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  小麦赤霉病(Fusarium head blightFHB)主要由禾谷镰孢菌(Fusarium graminearumFg)引起的一种严重危害小麦生产的真菌病害。偃麦草(Elymus repens, 2n=6x=42StStStHH)是小麦的野生近缘种,具有许多抗生物和非生物胁迫的优良性状。为了将该野生种质的抗赤霉病基因转移并应用于小麦育种,我们从偃麦草与普通小麦杂交的衍生后代中鉴定了一个新的抗赤霉病易位系K140-7通过基因组原位杂交(GISH)、非变性-荧光原位杂交(ND-FISH)、寡核苷酸-FISH涂染(Oligo-FISH painting)、拷贝基因-FISH(single gene FISH)和小麦55K SNP芯片分析发现K140-7是一个细胞遗传学稳定的7DS·7StL易位系,易位断点(340.8-342.5 Mb)靠近染色体7D的着丝粒区域336.3-341.7 Mb)。基于二倍体鹅观草Pseudoroegneria libanotica)St参考基因组,开发了217StL特异简单序列重复(SSR分子标记。不同小麦背景中对7DS·7StL易位进行基因型和表型分析,表明外源片段7StL赋予了小麦赤霉病抗性且抗病性为显性,将该赤霉病抗性位点命名为QFhb.Er-7StL进一步将该位点向种遗传背景的小麦品种进行转移,创制了3份农艺性状更为优良的第二代7DS·7StL易位系,可用于小麦赤霉病抗性遗传改良。

Abstract  Fusarium head blight (FHB), mainly caused by Fusarium graminearum (Fg), is one of the most devastating fungal diseases in wheat production worldwide.  Elymus repens (2n=6x=42, StStStStHH) is a wild relative of wheat with many biotic and abiotic stress resistance traits.  To transfer and apply the wild germplasm's resistance gene (s) for wheat breeding, we identified a new translocation line K140-7 with high resistance to FHB, developed from the derivative progenies of E. repens crossed with common wheat cultivars.  Cytogenetic analyses based on genomic in situ hybridization (GISH), non-denaturing fluorescence in situ hybridization (ND-FISH), oligonucleotide-FISH painting (Oligo-FISH painting), and single-gene FISH revealed that K140-7 had 40 wheat chromosomes and two 7DS·7StL translocated chromosomes.  Wheat 55K SNP array analysis confirmed that the translocated breakpoint (340.8~342.5 Mb) was close to the centromere region of chromosome 7D (336.3~341.7 Mb), supporting the 7DS·7StL translocation event.  Based on the diploid reference St genome of Pseudoroegneria libanotica, we developed 21 simple sequence repeats (SSR) markers, specific for chromosome arm 7StL. Genotyping and phenotyping analysis of the 7DS·7StL translocation in different wheat backgrounds demonstrated that the chromosome arm 7StL confers FHB resistance and possesses the dominant FHB resistance locus (s) named QFhb.Er-7StL.  We further transferred QFhb.Er-7StL into three different wheat cultivars, their second 7DS·7StL translocation line-generations showed improved agronomic traits, representing new germplasms that could be used in wheat FHB-resistant breeding programs.
Keywords:  Fusarium       head blight (FHB)              Elymus       repens              wheat              translocation line              breeding transfer  
Online: 03 April 2024  
About author:  Houyang Kang, Tel: +86-28-86291177, E-mail: houyang.kang@sicau.edu.cn; Dandan Wu, Tel: +86-28-82650350, E-mail: wudandan@sicau.edu.cn * These authors contributed equally to this work.

Cite this article: 

Fei Wang, Xin Zhao, Xianghai Yu, Wei Zhu, Lili Xu, Yiran Cheng, Yazhou Zhang, Yi Wang, Jian Zeng, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou, Dandan Wu, Houyang Kang. 2024. Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2024.03.027

Bai G H, Su Z Q, Cai J. 2018. Wheat resistance to Fusarium head blight. Canadian Journal of Plant Pathology, 40, 336–346.

Bi Y F, Zhao Q Z, Yan W K, Li M X, Liu Y X, Cheng C Y, Zhang L, Yu X Q, Li J, Qian C T, Wu Y F, Cheng J F, Lou Q F. 2020. Flexible chromosome painting based on multiplex PCR of oligonucleotides and its application for comparative chromosome analyses in Cucumis. The Plant Journal, 102, 178–186.

Buerstmayr M, Wagner C, Nosenko T, Omony J, Steiner B, Nussbaumer T, Mayer K F X, Buerstmayr H. 2021. Fusarium head blight resistance in European winter wheat: Insights from genome-wide transcriptome analysis. BMC Genomics, 22, 470.

Cainong J C, Bockus W W, Feng Y G, Chen P D, Qi L L, Sehgal S K, Danilova T V, Koo D H, Friebe B, Gill B S. 2015. Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theoretical and Applied Genetics, 128, 1019–1027.

Chai Y, Senay S, Horvath D, Pardey P. 2022. Multi-peril pathogen risks to global wheat production: A probabilistic loss and investment assessment. Frontiers in Plant Science, 13, 1034600.

Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y H, Xia R. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13, 1194–1202. 

Chen N, Chen W J, Yan H, Wang Y, Kang H Y, Zhang H Q, Zhou Y H, Sun G L, Sha L N, Fan X. 2020. Evolutionary patterns of plastome uncover diploid-polyploid maternal relationships in Triticeae. Molecular Phylogenetics and Evolution, 149, 106838.

Cui L, Ren Y K, Murray T D, Yan W Z, Guo Q, Niu Y Q, Sun Y, Li H J. 2018. Development of perennial wheat through hybridization between wheat and wheatgrasses: A review. Engineering, 4, 507–513.

Czaban J, Wróblewska B, Sułek A, Mikos M, Boguszewska E, Podolska G, Nieróbca A. 2015. Colonisation of winter wheat grain by Fusarium spp. and mycotoxin content as dependent on a wheat variety, crop rotation, a crop management system and weather conditions. Food Additives & Contaminants (Part A), 32, 874–910.

Danilova T V, Friebe B, Gill B S. 2014. Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. Theoretical and Applied Genetics, 127, 715–730.

Dewey D R. 1984. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson J P, ed., Gene Manipulation in Plant Improvement. Springer, Boston. pp. 209–279.

Du H M, Tang Z X, Duan Q, Tang S Y, Fu S L. 2018. Using the 6RLKu minichromosome of rye (Secale cereale L.) to create wheat-rye 6D/6RLKu small segment translocation lines with powdery mildew resistance. International Journal of Molecular Sciences, 19, 3933.

Du X, Feng X B, Li R X, Jin Y L, Shang L H, Zhao J X, Wang C Y, Li T D, Chen C H, Tian Z R, Deng P C, Ji W Q. 2022. Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight. Frontiers in Plant Science, 13, 1012939.

Endo T R. 1979. Selective gametocidal action of a chromosome of Aegilops cylindrica in a cultivar of common wheat. Wheat Information Service, 50, 24–28.

Endo T R, Tsunewaki K. 1975. Sterility of common wheat with Aegilops triuncialis cytoplasm. Journal of Heredity, 66, 13–18.

Fedak G, Cao W, Wolfe D, Chi D, Xue A. 2017. Molecular characterization of Fusarium resistance from Elymus repens introgressed into bread wheat. Cytology and Genetics, 51, 130–133.

Gamache J, Sun G L. 2015. Phylogenetic analysis of the genus Pseudoroegneria and the Triticeae tribe using the rbcL gene. Biochemical Systematics and Ecology, 62, 73–81.

Gilbert J, Haber S. 2013. Overview of some recent research developments in Fusarium head blight of wheat. Canadian Journal of Plant Pathology, 35, 149–174.

Gong B R, Zhu W, Li S Y, Wang Y Q, Xu L L, Wang Y, Zeng J, Fan X, Sha L N, Zhang H Q, Qi P F, Huang L, Chen G Y, Zhou Y H, Kang H Y. 2019. Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust. BMC Plant Biology, 19, 590.

Guo X R, Shi Q H, Liu Y, Su H D, Zhang J, Wang M, Wang C H, Wang J, Zhang K B, Fu S L, Hu X J, Jing D L, Wang Z, Li J B, Zhang P Z, Liu C, Han F P. 2023. Systemic development of wheat-Thinopyrum elongatum translocation lines and their deployment in wheat breeding for Fusarium head blight resistance. The Plant Journal, 114, 1475–1489.

Guo X R, Wang M, Kang H Y, Zhou Y H, Han F P. 2022. Distribution, polymorphism and function characteristics of the GST-encoding Fhb7 in Triticeae. Plants, 11, 2074.

Han F P, Lamb J C, Birchler J A. 2006. High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proceedings of the National Academy of Sciences of the United States of America, 103, 3238–3243.

Hu W J, Fu L P, Gao D R, Li D S, Liao S, Lu C B. 2023. Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in the high-quality soft wheat cultivar Yangmai 15. Journal of Integrative Agriculture, 22, 360–370,

Jia H W, Feng H, Yang G T, Li H W, Fu S L, Li B, Li Z S, Zheng Q. 2022. Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430. Theoretical and Applied Genetics, 135, 3277–3291.

Jia H Y, Zhou J Y, Xue S L, Li G Q, Yan H S, Ran C F, Zhang Y D, Shi J X, Jia L, Wang X, Luo J, Ma Z Q. 2018. A journey to understand wheat Fusarium head blight resistance in the Chinese wheat landrace Wangshuibai. The Crop Journal, 6, 48–59.

Juliana P, He X Y, Kabir M R, Roy K K, Anwar M B, Marza F, Poland J, Shrestha S, Singh R P, Singh P K. 2020. Genome-wide association mapping for wheat blast resistance in CIMMYT's international screening nurseries evaluated in Bolivia and Bangladesh. Scientific Reports, 10, 15972.

Karlsson I, Friberg H, Kolseth A K, Steinberg C, Persson P. 2017. Agricultural factors affecting Fusarium communities in wheat kernels. International Journal of Food Microbiology, 252, 53–60.

Li G R, Zhang T, Yu Z H, Wang H J, Yang E N, Yang Z J. 2020. An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae. The Plant Journal, 105, 978–993.

Li H W, Zhang F P, Zhao J X, Bai G H, Amand P S, Bernardo A, Ni Z F, Sun Q X, Su Z Q. 2022. Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse. Theoretical and Applied Genetics, 135, 1867–1877.

Li J F, Miao B B, Wang S X, Dong W, Xu H X, Si C C, Wang W, Duan S Q, Lou J C, Bao Z W, Zeng H L, Yang Z Z, Cheng W Y, Zhao F, Zeng J M, Liu X S, Wu R, Shen Y, Chen Z, Chen S J, et al. 2022. Hiplot: A comprehensive and easy-to-use web service for boosting publication-ready biomedical data visualization. Briefings in Bioinformatics, 23, bbac261.

Li J J, Li Z, Lü B Y, Fu Y, Zhang S F, Liu S H, Yang Q H, Wu J, Li J C, Chen X H. 2023. Development and characterization of a novel common wheat-Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance. Journal of Integrative Agriculture, 22, 1291–1307.

Li P Y. 2010. Turf-using germplasm resource evaluation on native Elytrigia repens (L.) Nevski in Xinjiang. Ph. D. dissertation, Xinjiang Agricultural University. (in Chinese)

Liu C, Fels-Klerx H J V D. 2021. Quantitative modeling of climate change impacts on mycotoxins in cereals: A review. Toxins, 13, 276.

Liu C, Han R, Wang X L, Gong W P, Cheng D G, Cao X Y, Liu A F, Li H S, Liu J J. 2020. Research progress of wheat wild hybridization, disease resistance genes transfer and utilization. Scientia Agricultura Sinica, 53, 1287–1308. (in Chinese)

Ma Z Q, Xie Q, Li G Q, Jia H Y, Zhou J Y, Kong Z X, Li N, Yuan Y. 2020. Germplasms, genetics and genomics for better control of disastrous wheat Fusarium head blight. Theoretical and Applied Genetics, 133, 1541–1568.

McMullen M, Bergstrom G, Wolf E D, Dill-Macky R, Hershman D, Shaner G, Sanford D V. 2012. A unified effort to fight an enemy of wheat and barley: Fusarium head blight. Plant Disease, 96, 1712–1728.

Mujeeb-Kazi A, Kazi A G, Dundas I, Rasheed A, Ogbonnaya F, Kishii M, Bonnett D, Wang R R C, Xu S, Chen P D, Mahmood T, Bux H, Farrakh S. 2013. Genetic diversity for wheat improvement as a conduit to food security. In: Sparks D L ed., Advances in Agronomy. Academic Press, San Diego. pp. 179–257.

Niranjana M. 2017. Gametocidal genes of Aegilops: Segregation distorters in wheat-Aegilops wide hybridization. Genome, 60, 639–647.

Oliver R E, Cai X, Xu S S, Chen X, Stack R W. 2005. Wheat‐alien species derivatives: A novel source of resistance to Fusarium head blight in wheat. Crop Science, 45, 1353–1360.

Qi L L, Pumphrey M O, Friebe B, Chen P D, Gill B S. 2008. Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theoretical and Applied Genetics, 117, 1155–1166.

Shen X R, Ohm H. 2007. Molecular mapping of Thinopyrum-derived Fusarium head blight resistance in common wheat. Molecular Breeding, 20, 131–140.

Song R R, Cheng Y F, Wen M X, Song X Y, Wang T, Xia M S, Sun H J, Cheng M H, Cui H M, Yuan C X, Liu X X, Wang Z K, Sun L, Wang H Y, Xiao J, Wang X E. 2023. Transferring a new Fusarium head blight resistance locus FhbRc1 from Roegneria ciliaris into wheat by developing alien translocation lines. Theoretical and Applied Genetics, 136, 36.

Song X Y, Song R R, Zhou J W, Yan W K, Zhang T, Sun H J, Xiao J, Wu Y F, Xi M L, Lou Q F, Wang H Y, Wang X E. 2020. Development and application of oligonucleotide-based chromosome painting for chromosome 4D of Triticum aestivum L. Chromosome Research, 28, 171–182.

Tan B W, Wang M M, Cai L, Li S Y, Zhu W, Xu L L, Wang Y, Zeng J, Fan X, Sha L N, Wu D D, Cheng Y R, Zhang H Q, Chen G Y, Zhou Y H, Kang H Y. 2022. Cytogenetic and molecular marker analyses of a novel wheat-Psathyrostachys huashanica 7Ns disomic addition line with powdery mildew resistance. International Journal of Molecular Sciences, 23, 10285.

Tang Z X, Yang Z J, Fu S L. 2014. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics, 55, 313–318.

Wan Y F, Yen C, Yang J L. 1997. The diversity of head-scab resistance in Triticeae and their relation to ecological conditions. Euphytica, 97, 277–281.

Wang H, Cheng S W, Shi Y, Zhang S X, Yan W, Song W F, Yang X F, Song Q J, Jang B, Qi X Y, Li X L, Friebe B, Zhang Y M. 2021. Molecular cytogenetic characterization and Fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids. Molecular Cytogenetics, 14, 15.

Wang H W, Sun S L, Ge W Y, Zhao L F, Hou B Q, Wang K, Lyu Z F, Chen L Y, Xu S S, Guo J, Li M, Su P S, Li X F, Wang G P, Bo C Y, Fang X J, Zhuang W W, Cheng X X, Wu J W, Dong L H, et al. 2020. Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science, 368, eaba5435.

Wang J, Liu Y L, Su H D, Guo X R, Han F P. 2017. Centromere structure and function analysis in wheat-rye translocation lines. The Plant Journal, 91, 199–207. 

Wang R R C, Li X F, Robbins M D, Larson S R, Bushman S B, Jones T A, Thomas A. 2020. DNA sequence-based mapping and comparative genomics of the St genome of Pseudoroegneria spicata (Pursh) Á. Löve versus wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). Genome, 63, 445–457.

Wang S W, Wang C Y, Feng X B, Zhao J X, Deng P C, Wang Y J, Zhang H, Liu X L, Li T D, Chen C H, Wang B T, Ji W Q. 2022. Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines. BMC Plant Biology, 22, 111.

Wang X, Li G Q, Jia H Y, Cheng R, Zhong J K, Shi J X, Chen R T, Wen Y X, Ma Z Q. 2024. Breeding evaluation and precise mapping of Fhb8 for Fusarium head blight resistance in wheat (Triticum aestivum). Plant Breeding, 143, 26-33.

Wu D D, Yang N M, Xiang Q, Zhu M K, Fang Z Y, Zheng W, Lu J L, Sha L N, Fan X, Cheng Y R, Wang Y, Kang H Y, Zhang H Q, Zhou Y H. 2022. Pseudorogneria libanotica intraspecific genetic polymorphism revealed by fluorescence in situ hybridization with newly identified tandem repeats and wheat single-copy gene probes. International Journal of Molecular Sciences, 23, 14818.

Xiao J, Cheng Y F, Song R R, Sun L, Wang Z K, Yuan C X, Wang H Y, Wang X E. 2021. Creation and utilization of resistant wheat alien germplasms to Fusarium head blight. Current Biotechnology, 11, 560–566. (in Chinese)

Xing L P, Yuan L, Lv Z S, Wang Q, Yin C H, Huang Z P, Liu J Q, Cao S Q, Zhang R Q, Chen P D, Karafiátová M, Vrána J, Bartoš J, Doležel J, Cao A Z. 2021. Long-range assembly of sequences helps to unravel the genome structure and small variation of the wheat-Haynaldia villosa translocated chromosome 6VS.6AL. Plant Biotechnology Journal, 19, 1567–1578.

Xu X M, Nicholson P. 2009. Community ecology of fungal pathogens causing wheat head blight. Annual Review of Phytopathology, 47, 83–103.

Yang G T, Tong C Y, Li H W, Li B, Li Z S, Zheng Q. 2022. Cytogenetic identification and molecular marker development of a novel wheat-Thinopyrum ponticum translocation line with powdery mildew resistance. Theoretical and Applied Genetics, 135, 2041–2057. 

Yang X Y, Xu M R, Wang Y F, Cheng X F, Huang C X, Zhang H, Li T D, Wang C Y, Chen C H, Wang Y J, Ji W Q. 2022. Development and molecular cytogenetic identification of two wheat-Aegilops geniculate Roth 7Mg chromosome substitution lines with resistance to Fusarium head blight, powdery mildew and stripe rust. International Journal of Molecular Sciences, 23, 7056.

Yen C, Yang J L. 2022. Biosystematics of genus Elymus. In: Biosystematics of Triticeae: Volume V. Springer, Singapore. pp. 67-416.

Zeng J, Cao W, Hucl P, Yang Y, Xue A, Chi D, Fedak G. 2013. Molecular cytogenetic analysis of wheat-Elymus repens introgression lines with resistance to Fusarium head blight. Genome, 56, 75–82.

Zhu Z W, Hao Y F, Mergoum M, Bai G H, Humphreys G, Cloutier S, Xia X C, He Z H. 2019. Breeding wheat for resistance to Fusarium head blight in the Global North: China, USA, and Canada. The Crop Journal, 7, 730–738.

No related articles found!
No Suggested Reading articles found!