Please wait a minute...
Journal of Integrative Agriculture  2024, Vol. 23 Issue (02): 731-734    DOI: 10.1016/j.jia.2023.11.042
Letter Advanced Online Publication | Current Issue | Archive | Adv Search |
First identification of the oxazolidinone/phenicol resistance gene optrA in Streptococcus pluranimalium worldwide

Kuan Zhao1*, Longyu Zhou2, 3*, Shixia Zhang1, Wanjiang Zhang2#, Yao Zhu2#

1 College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China

2 State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

3 College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

恶唑烷酮类抗生素作为目前治疗多重耐药革兰氏阳性菌感染最有效的抗生素之一,其耐药基因optrA的流行和传播备受关注,然而,目前optrA基因在链球菌中的流行情况尚不十分清楚。本文从山东省某猪场分离出一株多发性链球菌(Streptococcus pluranimalium S. pluranimaliumSP28,通过肉汤稀释法测定了该分离株的药物敏感性,其次利用IlluminaNanopore测序技术测定了该分离株的全基因组序列,通过生物信息学技术对全基因组序列进行比对分析,最后利用PCR方法检测插入序列IS1216E介导的易位单元(Translocatable UnitTU)。研究结果显示,分离株SP28表现出多重耐药表型和相对高的利奈唑胺MIC值。全基因组测序结果表明,成功获得了该分离株的完整染色体序列和一个质粒序列。耐药基因检测分析发现,该分离株的染色体携带optrA基因以及多个介导其它类型抗生素的耐药基因。耐药基因环境分析显示optrAfexA所在的耐药区域侧翼为两个同向的IS1216E插入序列,PCR检测结果显示携带optrA的耐药区域能够利用TU的方式进行水平转移,进而导致optrA的广泛传播。本研究首次在S. pluranimalium中发现耐药基因optrA,同时进一步揭示了IS1216EoptrA基因转移中发挥的重要作用。



Received: 23 June 2023   Accepted: 30 October 2023
CLC Number:   
Fund: This work was supported by the National Key Research and Development Program of China (grant no. 2022YFF0710505). The central Public-interest Scientific Institution Basal Research Fund (grant no. 1610302022001) and the Talents Introduction Projects of Hebei Agricultural University (grant no. YJ201945).
About author:  ZHAO Kuan, Fax: +98-0312-7528377, E-mail: zhaokuan519@126.com; ZHOU Long-yu, Tel: E-mail: zly6987@163.com; #Correspondence ZHANG Wan-jiang, Tel: +86-451-51051734, Fax: +86-0451-51997166, E-mail: zhangwanjiang@caas.cn; ZHU Yao, Tel: +86-451-51051734, Fax: +86-0451-51997166, E-mail: yaozhu922@163.com *These authors contributed equally to the work.

Cite this article: 

Kuan Zhao, Longyu Zhou, Shixia Zhang, Wanjiang Zhang, Yao Zhu. 2024. First identification of the oxazolidinone/phenicol resistance gene optrA in Streptococcus pluranimalium worldwide . Journal of Integrative Agriculture, 23(02): 731-734.

Aziz R K, Bartels D, Best A A, DeJongh M, Disz T, Edwards R A, Formsma K, Gerdes S, Glass E M, Kubal M, Meyer F, Olsen G J, Olson R, Osterman A L, Overbeek R A, McNeil L K, Paarmann D, Paczian T, Parrello B, Pusch G D, et al. 2008. The RAST Server: Rapid annotations using subsystems technology. BMC Genomics, 9, 75.

Bortolaia V, Kaas R S, Ruppe E, Roberts M C, Schwarz S, Cattoir V, Philippon A, Allesoe R L, Rebelo A R, Florensa A F, Fagelhauer L, Chakraborty T, Neumann B, Werner G, Bender J K, Stingl K, Nguyen M, Coppens J, Xavier B B, Malhotra-Kumar S, et al. 2020. ResFinder 4.0 for predictions of phenotypes from genotypes. Journal of Antimicrobial Chemotherapy, 75, 3491–3500.

Brenciani A, Fioriti S, Morroni G, Cucco L, Morelli A, Pezzotti G, Paniccia M, Antonelli A, Magistrali C F, Rossolini G M, Giovanetti E. 2019. Detection in Italy of a porcine Enterococcus faecium isolate carrying the novel phenicol-oxazolidinone-tetracycline resistance gene poxtA. Journal of Antimicrobial Chemotherapy, 74, 817–818.

Brenciani A, Morroni G, Schwarz S, Giovanetti E. 2022. Oxazolidinones: Mechanisms of resistance and mobile genetic elements involved. Journal of Antimicrobial Chemotherapy, 77, 2596–2621.

CLSI (Clinical and Laboratory Standards Institute). 2022. Performance Standards for Antimicrobial Susceptibility Testing. Thirty-Second Informational Supplement. Clinical and Laboratory Standards Institute Document M100-S32. Clinical and Laboratory Standards Institute, Wayne, PA.

Fu D J, Ramachandran A, Miller C. 2021. Streptococcus pluranimalium meningoencephalitis in a horse. Journal of Veterinary Diagnostic Investigation, 33, 956–960.

Harmer C J, Moran R A, Hall R M. 2014. Movement of IS26-associated antibiotic resistance genes occurs via a translocatable unit that includes a single IS26 and preferentially inserts adjacent to another IS26. mBio, 5, e01801–e01814.

Pan Y, An H, Fu T, Zhao S, Zhang C, Xiao G, Zhang J, Zhao X, Hu G. 2018. Characterization of Streptococcus pluranimalium from a cattle with mastitis by whole genome sequencing and functional validation. BMC Microbiology, 18, 182.

Schwarz S, Zhang W, Du X D, Kruger H, Fessler A T, Ma S, Zhu Y, Wu C, Shen J, Wang Y. 2021. Mobile oxazolidinone resistance genes in Gram-positive and Gram-negative bacteria. Clinical Microbiology Reviews, 34, e0018820.

Sharkey L K, Edwards T A, O’Neill A J. 2016. ABC-F proteins mediate antibiotic resistance through ribosomal protection. mBio, 7, e01975.

Wang Y, Lv Y, Cai J, Schwarz S, Cui L, Hu Z, Zhang R, Li J, Zhao Q, He T, Wang D, Wang Z, Shen Y, Li Y, Fessler A T, Wu C, Yu H, Deng X, Xia X, Shen J. 2015. A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin. Journal of Antimicrobial Chemotherapy, 70, 2182–2190.

Wick R R, Judd L M, Gorrie C L, Holt K E. 2017. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Computational Biology, 13, e1005595.

Yang Q, Zhu Y, Schwarz S, Wang L, Liu W, Yang W, Liu S, Zhang W. 2023. Integrative and conjugative elements in streptococci can act as vectors for plasmids and translocatable units integrated via IS1216E. International Journal of Antimicrobial Agents, 61, 106793.

Zhu Y, Zhang W, Liu S, Schwarz S. 2021. Identification of an IS431-derived translocatable unit containing the erm(C) gene in Staphylococcus aureus. Journal of Antimicrobial Chemotherapy, 76, 1102–1104.

No related articles found!
No Suggested Reading articles found!