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| New geographic distribution and molecular diversity of Citrus chlorotic dwarf-associated virus in China |
| YANG Zhen1*, ZHANG Lan2*, ZHAO Jin-fa1, ZHANG Xing-kai1, WANG Ying1, LI Tai-sheng1, ZHANG Wei3, ZHOU Yan1 |
1 National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
2 Horticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530000, P.R.China
3 Plant Protection Station of Sichuan Agriculture and Rural Bureau, Chengdu 610041, P.R.China
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摘要
2009年在中国首次发现了柑橘褪绿矮缩病毒（CCDaV），该病毒在云南瑞丽造成柠檬大量发病、减产，损失严重。目前，CCDaV在中国其它柑橘种植省份的发生分布和流行趋势尚不清楚。为了系统掌握CCDaV在中国的发生分布及其分子特性，本研究于2017-2019年，从中国11个柑橘主要生产省份采集了1,772份柑橘样品，通过PCR检测发现，采自广西省、云南省和广东省的134份柑橘样品感染了CCDaV。其中感病品种分别为红宝石蜜柚（50.8%）、泰国青柚（24.0%）、尤力克柠檬（20.8%）、墨西哥莱檬（20.0%）、塔希提莱檬（18.2%）和三红蜜柚（3.2%）。在采集的甜橙、宽皮柑橘、杂柑、枳、葡萄柚和金柑等柑橘类型样品中均没有检测出CCDaV。与前期的研究结果相比，CCDaV在中国的发生区域正在逐步扩大，且感病品种的种类也在增加。此外，CCDaV在红宝石蜜柚、泰国青柚和三红蜜柚新梢上产生的症状较其在尤力克柠檬上更为严重，除产生典型的“V”型叶，叶片扭曲、畸形，黄化外，CCDaV在上述3种柚类品种上还能产生严重的脉明症状。本研究选取了17个CCDaV毒株与GenBank数据库中已知的15个CCDaV毒株进行全系列分析，结果显示CCDaV的序列保守性高，所有 32个CCDaV毒株的核苷酸相似性大约为99%-100%。系统发育树分析表明，CCDaV毒株间的亲缘关系与其采样地和寄主存在相关性。根据地理来源和寄主种类的差异，CCDaV毒株被划分成了4个不同的类群，其中中国和土耳其的CCDaV毒株属于不同的类群。此外，还首次证明中国的CCDaV毒株可能存在多个起源中心，且部分毒株可能来自泰国。本研究结果为明确CCDaV在中国的发生分布、流行规律，以及遗传演化提供了重要的理论依据。
Abstract In 2009, an emerging citrus viral disease caused by Citrus chlorotic dwarf-associated virus (CCDaV) was discovered in Yunnan Province of China. However, the occurrence and spread of CCDaV in other citrus-growing provinces in China is unknown to date. To better understand the distribution and molecular diversity of CCDaV in China, a total of 1 772 citrus samples were collected from 11 major citrus-growing provinces and were tested for CCDaV by PCR. Among these, 134 citrus samples from Guangxi, Yunnan and Guangdong were tested positive for CCDaV, demonstrating that the occurrence and spread of CCDaV are increasing in China. The complete genome sequences of 17 CCDaV isolates from different provinces and hosts were sequenced. Comparisons of the whole-genome sequences of the 17 CCDaV isolates as well as the 15 isolates available in GenBank revealed that the sequence identity was about 99–100%, showing that the CCDaV isolates were highly conserved. Phylogenetic studies showed that the 32 CCDaV isolates belonged to four different groups based on geographical origins and host species, and that CCDaV isolates from China and Turkey were clustered into different groups. The results provide important information for clarifying the distribution and genetic diversity of CCDaV in China.
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Received: 19 September 2020
Accepted: 18 December 2020
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| Fund: This research was partially supported by the National Key R&D Program of China (2019YFD1001800), the China Agriculture Research System, Overseas Expertise Introduction Project for Discipline Innovation (B18044), the China Agriculture Research System of MOF and MARA (CARS-26-05B), the Natural Science Foundation of Chongqing, China (cstc2019jcyj-msxmX0557), and the Guangxi Natural Science Foundation, China (2018GXNSFBA050027). |
| About author: YANG Zhen, Tel: +86-23-68349002, E-mail: 1374960920@qq.com; Correspondence ZHOU Yan, E-mail: zhouyan@cric.cn
* These authors contributed equally to this study. |
Cite this article:
YANG Zhen, ZHANG Lan, ZHAO Jin-fa, ZHANG Xing-kai, WANG Ying, LI Tai-sheng, ZHANG Wei, ZHOU Yan.
2022.
New geographic distribution and molecular diversity of Citrus chlorotic dwarf-associated virus in China. Journal of Integrative Agriculture, 21(1): 293-298.
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