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Special Issue:
玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources
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| Creation of two hyperactive variants of phytochrome B1 for attenuating shade avoidance syndrome in maize |
| ZHAO Yong-ping1*, ZHAO Bin-bin1*, WU Guang-xia1*, MA Xiao-jing1, WANG Bao-bao1, KONG De-xin2, WEI Hong-bin2, WANG Hai-yang2,3 |
1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Ministry of Science and Technology/South China Agricultural University, Guangzhou 510642, P.R.China
3 Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, P.R.China
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摘要
增加种植密度是提高玉米产量的有效措施。然而，密植容易引发植物的避荫反应综合征，导致抗倒伏能力下降，最终导致产量降低。光敏色素B（phyB）在植物避荫反应中起主导作用。研究表明，将拟南芥PHYB(AtPHYB)104和361位的酪氨酸(Y)改变为苯丙氨酸(F)可以增强其活性。在本研究中，我们通过模拟AtPHYB的Y104F和Y361F的突变形式，创制了两个玉米PHYB1超敏突变体: ZmPHYB1Y98F和ZmPHYB1Y359F。在ZmPHYB1自身启动子的驱动下，将ZmPHYB1Y98F、ZmPHYB1Y359F和野生型的ZmPHYB1 (ZmPHYB1WT)转入到拟南芥phyB-9突变体中。在转基因拟南芥中，与ZmPHYB1WT相比，异源表达ZmPHYB1Y98F和ZmPHYB1Y359F增强了对phyB-9相关表型的互补功能。与ZmPHYB1WT相似，红光处理可诱导ZmPHYB1Y98F和ZmPHYB1Y359F蛋白从细胞质中转运到细胞核，并且能够与玉米中的PIF蛋白互作。此外，在模拟遮荫处理下，ZmPHYB1自身启动子驱动的ZmPHYB1Y98F和ZmPHYB1Y359F在转基因玉米中的表达可以减弱玉米幼苗的避荫反应综合征，还能够降低玉米植株的株高和穗位高。综上所述，研究结果表明ZmPHYB1Y98F和ZmPHYB1Y359F能够减弱玉米的避荫反应综合征，为培育耐密玉米品种提供了新思路。
Abstract Increasing the planting density of maize is an effective measure to improve its yield. However, plants under high planting density tend to trigger shade avoidance syndrome (SAS), reducing lodging resistance and ultimately yield drop. Phytochrome B
(phyB) plays a dominant role in mediating shade avoidance response. This study constructed two hyperactive mutated alleles of maize PHYB1: ZmPHYB1Y98F (mimicking Y104F of AtPHYB) and ZmPHYB1Y359F (mimicking Y361F of AtPHYB). Ectopic expression of ZmPHYB1Y98F and ZmPHYB1Y359F under the control of the ZmPHYB1 promoter in the Arabidopsis phyB-9 background rendered enhanced activity on complementing the phyB-9 related phenotypes compared with ZmPHYB1WT. Moreover, similar to the behavior of ZmPHYB1WT, ZmPHYB1Y98F and ZmPHYB1Y359F proteins are localized to the nucleus after red light exposure, and could interact with PIF proteins of maize. In addition, expression of ZmPHYB1Y98F and ZmPHYB1Y359F variants under the control of the native ZmPHYB1 promoter attenuated SAS of maize seedlings subjected to simulated shade treatment. It effectively reduced mature maize’s plant height and ear height in field conditions. The results combined demonstrate the utility of ZmPHYB1Y98F and ZmPHYB1Y359F for attenuating SAS and breeding high density-tolerant varieties of maize.
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Received: 15 August 2020
Accepted: 15 October 2020
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| Fund: This work was financially supported by the Major Program of Guangdong Basic and Applied Research, China (2019B030302006), the National Natural Science Foundation of China (31801377) and the funding from the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China (SKLCUSA-b201801). |
| About author: Correspondence WANG Hai-yang, Tel/Fax: +86-10-82105488, E-mail: whyang@scau.edu.cn
* These authors contributed equally to this study. |
Cite this article:
ZHAO Yong-ping, ZHAO Bin-bin, WU Guang-xia, MA Xiao-jing, WANG Bao-bao, KONG De-xin, WEI Hong-bin, WANG Hai-yang.
2022.
Creation of two hyperactive variants of phytochrome B1 for attenuating shade avoidance syndrome in maize. Journal of Integrative Agriculture, 21(5): 1253-1265.
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