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Journal of Integrative Agriculture  2020, Vol. 19 Issue (5): 1261-1273    DOI: 10.1016/S2095-3119(20)63171-9
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Genome-wide identification and expression analysis of asparagine synthetase family in apple
YUAN Xi-sen1*, YU Zi-peng1*, LIU Lin1, XU Yang2, ZHANG Lei1, HAN De-guo3, ZHANG Shi-zhong1 
1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2 Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, P.R.China
3 College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, P.R.China
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Asparagine is an efficient nitrogen transport and storage carrier.  Asparagine synthesis occurs by the amination of aspartate which is catalyzed by asparagine synthetase (ASN) in plants.  Complete genome-wide analysis and classifications of the ASN gene family have recently been reported in different plants.  However, systematic analysis and expression profiles of these genes have not been performed in apple (Malus domestica).  Here, a comprehensive bioinformatics approach was applied to identify MdASNs in apple.  Then, plant phylogenetic tree, chromosome location, conserved protein motif, gene structure, and expression pattern of MdASNs were analyzed.  Five members were identified and distributed on 4 chromosomes with conserved GATase-7 and ASN domains.  Expression analysis indicated that all MdASNs mRNA accumulated at the highest level in reproductive organs, namely flowers or fruits, which may be associated with the redistribution of free amino acids in plant metabolic organs and reservoirs.  Additionally, most of MdASNs were dramatically up-regulated under various nitrogen supplies, especially in the aboveground part.  Taken together, MdASNs may be assigned to be responsible for the nitrogen metabolism and asparagine synthesis in apple.
Keywords:  Malus domestica       bioinformatics        ASN family        asparagine synthetase        nitrogen metabolism  
Received: 17 May 2019   Accepted:
Fund: This study was financially supported by the National Natural Science Foundation of China (31901574 and 31570271).
Corresponding Authors:  Correspondence HAN De-guo, Tel/Fax: +86-451-55190243, E-mail:; ZHANG Shi-zhong, Tel/Fax: +86-538-8241318, E-mail:    
About author:  YUAN Xi-sen, E-mail:; YU Zi-peng, E-mail:; * These authors contributed equally to this study.

Cite this article: 

YUAN Xi-sen, YU Zi-peng, LIU Lin, XU Yang, ZHANG Lei, HAN De-guo, ZHANG Shi-zhong. 2020.

Genome-wide identification and expression analysis of asparagine synthetase family in apple
. Journal of Integrative Agriculture, 19(5): 1261-1273.

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