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Journal of Integrative Agriculture  2020, Vol. 19 Issue (1): 193-203    DOI: 10.1016/S2095-3119(19)62778-4
Special Issue: 昆虫合辑Plant Protection—Entomolgy 昆虫和植物互作合辑Insect and Plant Interact
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Exploiting push-pull strategy to combat the tea green leafhopper based on volatiles of Lavandula angustifolia and Flemingia macrophylla
HAN Shan-jie1*, WANG Meng-xin1*, WANG Yan-su1, WANG Yun-gang2, CUI Lin1, HAN Bao-yu1
1 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, P.R.China
2 Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, P.R.China
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Abstract  
Thirteen volatile compounds were identified from Flemingia macrophylla plants.  Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F.  Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, MeSA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL–1 in n-hexane, respectively; 2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL–1 in n-hexane, respectively; 3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and MeSA at 2, 10, 10 and 11 mg mL–1 in n-hexane, respectively.  Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL–1.  For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps.  The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards.  Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components.  When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 92 leafhoppers/trap/day.  Control efficacy on the leafhoppers within the push-pull zones increased progressively from day 1 (43%) to day 5 (73%).  This push-pull approach might have a great potential as a green control strategy for combating the tea green leafhoppers. 
Keywords:  push-pull strategy        attractants        repellents        Flemingia macrophylla        tea green leafhopper        tea plantation  
Received: 15 February 2019   Accepted:
Fund: The work was financially supported by the National Key Research and Development Program of China (2018YFC1604402), the Natural Science Foundation of Zhejiang Province, China (LY17C140002), the Fundamental and Public Welfare of Zhejiang Province, China (LGN18C160006), and the College Student Innovation and Entrepreneurship of Zhejiang Province, China (2017R409055).
Corresponding Authors:  Correspondence HAN Bao-yu, Tel: +86-571-86835706, E-mail: han-insect@263.net    
About author:  HAN Shan-jie, E-mail: hanshanjie@126.com; * These authors contributed equally to this study.

Cite this article: 

HAN Shan-jie, WANG Meng-xin, WANG Yan-su, WANG Yun-gang, CUI Lin, HAN Bao-yu. 2020. Exploiting push-pull strategy to combat the tea green leafhopper based on volatiles of Lavandula angustifolia and Flemingia macrophylla. Journal of Integrative Agriculture, 19(1): 193-203.

Cai X M, Li Z Q, Pan H S, Lv Y H. 2018. Research and application of food-based attractants of herbivorous insect pests. Chinese Journal of Biological Control, 34, 8–35. (in Chinese)
Cheng S Y, Xie Y, Feng X L, Huang L F. 2012. Study of the volatile constituents in radix Flemingiae macrophyllae and a substitute by gas chromatography-mass spectrometry and chemometric methods. Molecules, 17, 14111–14125.
Cook S M, Khan Z R, Pickett J A. 2007. The use of push-pull strategies in integrated pest management. Annual Review of Entomology, 52, 375–400.
Gregg P C, Del Socorrol A P, Hawes A J, Binns M R. 2016. Developing bisexual attract-and-kill for polyphagous insects. Journal of Chemical Ecology, 42, 666–675.
Han B Y, Chen Z M. 2002. Composition of the volatiles from intact and mechanically pierced tea aphid-tea shoot complexes and their attraction to natural enemies of the tea aphid. Journal of Agricultural and Food Chemistry, 50, 2571–2575.
Han S J, Pan C, Han B Y. 2016. Changes in volatiles of tea shoots damaged by tea green leafhoppers and their attraction to Schizophragma parvula Ogloblin. Chinese Journal of Biological Control, 32, 142–148. (in Chinese)
Jiang L R, Liu S A, Han B Y, Ouyang L M. 2010. Effect of odours from seven species of host and non-host plants on the adult behaviour of the tea geometrid, Ectropis obliqua (Prout). Acta Ecologica Sinica, 30, 4993–5000. (in Chinese)
Liu J H, Gao L X, Gao Y Q, Huo X. 2003. Study on the chemical constituents of volatile oil from radix Flemingiae Philippinensis. Chinese Traditional Patent Medicine, 25, 485–487. (in Chinese)
Miyazaki H, Otake J, Mitsuno H, Ozaki K, Kanzaki R, Chieng A C T, Hee A K W, Nishida R, Ono H. 2018. Functional characterization of olfactory receptors in the Oriental fruit fly Bactrocera dorsalis that respond to plant volatiles. Insect Biochemistry and Molecular Biology, 101, 32–46.
Mu D, Cui L, Ge J, Wang M X, Liu L F, Yu X P, Zhang Q H, Han B Y. 2012. Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper Empoasca vitis. Insect Science, 19, 229–238.
Navarro-Llopis V, Primo J, Vacas S. 2013. Efficacy of attract-and-kill devices for the control of Ceratitis capitata. Pest Management Science, 69, 478–482.
Njuguna P K, Murungi L K, Fombong A, Teal P E A, Beck J J, Torto B. 2018. Cucumber and tomato volatiles: Influence on attraction in the melon fly Zeugodacus cucurbitate (Diptera: Tephritidae). Journal of Agricultural and Food Chemistry, 66, 8504–8513.
Pan C. 2015. Efficacity of infochemicals of tea and lavender plant regulating behaviour of Empoasca vitis and Stethynium empoascae. MSc thesis, China Jiliang University, China. (in Chinese)
Piesik D, Pañka D, Delaney K J, Skoczek A, Lamparski R, Weaver D K. 2011. Cereal crop volatile organic compound induction after mechanical injury, beetle herbivory (Oulema spp.), or fungal infection (Fusarium spp.). Journal of Plant Physiology, 168, 878–886.
Pinto-Zevallos D M, Strapasson P, Zarbin P H G. 2016. Herbivore-induced volatile organic compounds emitted by maize: Electrophysiological responses in Spodoptera frugiperda females. Phytochemistry Letters, 16, 70–74.
Pu X Y, Feng M G. 2004. Efficacy of emulsifiable formulations of two entomopathogenic fungi against small green leafhoppers on tea plant. Chinese Journal of Applied Ecology, 15, 619–622. (in Chinese)
Reher T, Van Kerckvoorde V, Loes Verheyden, Wenseleers T, Beliën T, Bylemans D, Martens J A. 2019. Evaluation of hop (Humulus lupulus) as a repellent for the management of Drosophila suzukii. Crop Protection, 124, 1–6.
Sun H N, Zhang F, Chen S M, Guan Z Y, Jiang J F, Fang W M, Chen F D. 2015. Effects of aphid herbivory on volatile organic compounds of Artemisia annua and Chrysanthemum morifolium. Biochemical Systematics and Ecology, 60, 225–233.
Wang M X, Li H X, Wu W Z, Sun H C, Shi S P, Ding Y, Cao C H, Han B Y. 2016. Behavioral responses of Empoasca vitis Gothe to volatiles from tea shoots. Chinese Journal of Applied Entomology, 53, 507–515. (in Chinese)
Xu Q X, Hatt S, Lopes T, Zhang Y, Bodson B, Chen J, Francis F. 2017. A push-pull strategy to control aphids combines intercropping with semiochemical releases. Journal of Pest Science, 91, 93–103.
Yan D, Xia H B, Li C, Liu S, Li Y M, Liao D F, Lin L M, Wu P. 2016. Research progress on medicinal plants of Flemingia Roxb. ex W. T. Ait. Chinese Traditional and Herbal Drugs, 47, 4456–4471. (in Chinese)
Zhang S. 2016. Studies on effect of intercropping of Flemingia macrophylla in tea plantation on community diversity of arthropod and foliage damaged percentage by insects. MSc thesis, University of Chinese Academy of Sciences, China. (in Chinese)
Zhang Z Q, Luo Z X, Gao Y, Bian L, Sun X L, Chen Z M. 2014. Volatiles from non-host aromatic plants repel tea green leafhopper Empoasca vitis. Entomologia Experimentalis et Applicata, 153, 156–169.
Zheng Y C, Niu Y Q, Cui G L, Zhou N N, Zhang X T, Wang M X, Cui L, Zheng Y T, Han B Y. 2013. Community composition and phototaxis of insects in tea plantations in southern Jiangsu Province during late fall. Acta Ecologica Sinica, 33, 5017–5025. (in Chinese)
Zhu D H, Wang Y L, Huang L F. 2012. Analysis of the volatile chemical constituents in Moghania Macrophylla by gas chromatography-mass spectrometry. Chemical Production and Technology, 19, 37–39. (in Chinese)
Zhuang J X, Fu J W, Su Q Q, Li J Y, Zhan Z X. 2009. The regional diversity of resistance of tea green leafhopper, Empoasca vitis (Göthe), to insecticides in Fujian Province. Journal of Tea Science, 29, 154–158. (in Chinese)
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