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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 1010-1018    DOI: 10.1016/S2095-3119(19)62723-1
Special Issue: Triticeae Crops Physiology · Biochemistry · Cultivation · Tillage
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of sodium benzoate on growth and physiological characteristics of wheat seedlings under compound heavy metal stress
LIANG Pan-pan, ZHAO Chen, LIN Yuan, GENG Ji-jia, CHEN Yuan, CHEN De-hua, ZHANG Xiang
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Agricultural College, Yangzhou University, Yangzhou 225009, P.R.China
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Abstract  
In this study, we investigated the effect of exogenous sodium benzoate on wheat seedlings (Yangmai 16) grown under heavy metal stress.  The results showed that 2.4 mmol kg–1 of heavy metals significantly inhibited growth and delayed emergence of wheat seedlings.  Under compound heavy metal stress, application of 2–4 g L–1 sodium benzoate significantly increased (P<0.01) chlorophyll content and chlorophyll fluorescence parameters Fv/Fm and Fv/Fo of wheat, compared to the control (water treatment).  Further analysis showed that application of 2–4 g L–1 sodium benzoate alleviated osmotic stress by promoting the accumulation of osmolytes such as soluble proteins and free proline, increased the activity of superoxide dismutase (SOD) and reduced malondialdehyde content (MDA).  In contrast, higher concentrations of sodium benzoate solution (>6 g L–1) inhibited the growth of wheat seedlings and even caused damage to seedlings.  Correlation analysis showed that when the sodium benzoate concentration was in the range of 1.97–3.12 g L–1 (2016) and 1.58–3.27 g L–1 (2017), values of chlorophyll and its components, root activity, SOD activity, soluble protein, and free proline content were the highest.  When the sodium benzoate concentration was raised to 2.59 g L–1 (2016) or 3.02 g L–1 (2017), MDA content was the lowest.  Ultimately, exogenous sodium benzoate (2–4 g L–1) facilitates root development and improves the root activity of wheat seedlings grown under compound heavy metals stress, thereby effectively alleviating the damage of compound heavy metal stress in wheat seedlings.
Keywords:  sodium benzoate        compound heavy metals        wheat seedlings        physiological characteristics  
Received: 07 December 2018   Accepted: 04 March 2020
Fund: The work was supported by the National Key Research and Development Program of China (2017YFD0201306), the National Natural Science Foundation of China (31671613, 31471435), the Ph D Programs Foundation of Jiangsu Province, China (1601116C), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China, and the Top-Notch Academic Programs Project of Jiangsu Higher Education Institutions, China (PPZY2015A060).
Corresponding Authors:  Correspondence ZHANG Xiang, Tel/Fax: +86-514-87979357, E-mail: zhangxiang@yzu.edu.cn    
About author:  Received 7 December, 2018 Accepted 13 May, 2019 LIANG Pan-pan, Tel/Fax: +86-514-87979357, E-mail: 1474635982@qq.com;

Cite this article: 

LIANG Pan-pan, ZHAO Chen, LIN Yuan, GENG Ji-jia, CHEN Yuan, CHEN De-hua, ZHANG Xiang. 2020. Effects of sodium benzoate on growth and physiological characteristics of wheat seedlings under compound heavy metal stress. Journal of Integrative Agriculture, 19(4): 1010-1018.

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