Please wait a minute...
Journal of Integrative Agriculture  2019, Vol. 18 Issue (9): 2063-2071    DOI: 10.1016/S2095-3119(19)62603-1
Special Issue: 植物细菌真菌合辑Plant Bacteria/Fungus
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Combined application of Trichoderma harzianum SH2303 and difenoconazole-propiconazolein controlling Southern corn leaf blight disease caused by Cochliobolus heterostrophus in maize
WANG Shao-qing1, 2, 3*, MA Jia4*, WANG Meng1, 2, 3, WANG Xin-hua1, 2, 3, LI Ya-qian1, 2, 3, CHEN Jie1, 2, 3  
1 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
2 Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
3 State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
4 Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Southern corn leaf blight (SCLB) disease caused by Cochliobolus heterostrophus is one of the major threats to corn production worldwide.  The synergistic application of low toxic chemical fungicide and biocontrol agents could improve biocontrol stability and efficiency against plant diseases, which ultimately reduce use of chemical fungicide.  Trichoderma spp., well-known biocontrol fungi have been used to control some foliar diseases.  However, few works have been reported on synergistic application of chemical fungicide and Trichoderma against foliar diseases.  This study was aimed to investigate the control effect on the synergistic application of Trichoderma harzianum SH2303 and difenoconazole-propiconazole (DP) against SCLB.  Results showed that the synergistic application of DP and SH2303 reduced the leaf spot area compared to the control.  The efficacy of synergistic application of DP+SH2303 against SCLB could last for 15–20 d in pot trial under the greenhouse condition.  Under the natural field condition, maize treated with DP+DP and DP+SH2303 showed 60% control, which was higher than that of SH2303+DP (45%) and SH2303+SH2303 (35%).  All these treatments induced the synthesis of defense-related enzymes (phenylalanine ammonia lyase (PAL), catalase (CAT), and superoxide dismutase (SOD)) and the defence-related gene expression of SA pathway (PR1).  Taken together the in-vitro leaf test and field trial, the control of SCLB by synergistic application of DP+SH2303 was similar to that of DP+DP.  Among synergistic application, the sequential application of DP+SH2303 showed better control than the sequential application of SH2303+DP.  It was concluded that the synergistic application of chemical fungicide (DP) and biocontrol agent (T. harzianum SH2303) could be used to reduce the chemical fungicide and to reduce the SCLB diseases in maize, which provided alternative approach to realize an eco-friendly controlling of the foliar disease.
Keywords:  synergistic bio-control        chemical fungicide        Trichoderma harzianum        maize leaf spot disease  
Received: 12 September 2018   Accepted: 31 August 2019
Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0201108, 2017YFD0200901), the National Natural Science Foundation of China (31672072, 31872015, 31750110455), the earmarked fund for China Agriculture Research System (CARS-02), the Key National R&D Programs of China - Key International Intergovernmental Scientific and Technological Innovation Cooperation Projects (2017YFE0104900) and the Agriculture Research System of Shanghai, China (201710).
Corresponding Authors:  Correspondence CHEN Jie, Tel: +86-21-34206141, E-mail:    
About author:  WANG Shao-qing, E-mail:; MA Jia, E-mail:; * These authors contributed equally to this study.

Cite this article: 

WANG Shao-qing, MA Jia, WANG Meng, WANG Xin-hua, LI Ya-qian, CHEN Jie . 2019. Combined application of Trichoderma harzianum SH2303 and difenoconazole-propiconazolein controlling Southern corn leaf blight disease caused by Cochliobolus heterostrophus in maize. Journal of Integrative Agriculture, 18(9): 2063-2071.

Aebi H. 1984. Catalase in vitro. In Methods in Enzymology, 105, 121–126.
Bhagyashree K, Beura S K. 2014. Efficacy of biocontrol agents against maydis leaf blight of maize. Journal of Plant Protection and Environment, 11, 95–97.
Bringel F, Couee I. 2018. Plant-pesticide interactions and the global chloromethane budget. Trends in Plant Science, 23, 95–99.
Elad Y, Freeman S. 2002. Biological control of fungal plant pathogens. In: Agricultural Applications. Springer, Berlin, Heidelberg. pp. 93–109.
Gao J X, Chen J. 2018. Transcriptome analysis identifies candidate genes associated with melanin and toxin biosynthesis and pathogenicity of the maize pathogen, Curvularia lunata. Journal of Phytopathology, 166, 233–241.
Hemm M R, Rider S D, Ogas J, Murry D J, Chapple C. 2004. Light induces phenylpropanoid metabolism in Arabidopsis roots. The Plant Journal, 38, 765–778.
Hirooka T, Ishii H. 2013. Chemical control of plant diseases. Journal of General Plant Pathology, 79, 390–401.
Howell C R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87, 4–10.
Hu X, Roberts D P, Xie L, Yu C, Li Y, Qin L, Hu L, Zhang Y, Liao X. 2016. Use of formulated Trichoderma sp. Tri-1 in combination with reduced rates of chemical pesticide for control of Sclerotinia sclerotiorium on oilseed rape. Crop Protection, 79, 124–127.
Kapat A, Zimand G, Elad Y. 1998. Effect of two isolates of Trichoderma harzianumon the activity of hydrolytic enzymes produced by Botrytis cinerea. Physiological and Molecular Plant Pathology, 52, 127–137.
Korolev N, David D R, Elad Y. 2008. The role of phytohormones in basal resistance and Trichoderma-induced systemic resistance to Botrytis cinerea in Arabidopsis thaliana. BioControl, 53, 667–683.
Ku?niak E, Sk?odowska M. 2005. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta, 222, 192–200.
Legrand F, Picot A, Cobo-Díaz J F, Chen W, Le Floch G. 2017. Challenges facing the biological control strategies for the management of Fusarium head blight of cereals caused by F. graminearum. Biological Control, 113, 26–38.
Li Q, Yan F, Chen L, He Y. 2011. Study on two methods of chemical control for maize gray spot disease. Journal of Yunnan Agricultural University (Natural Science Edition), 26, 735–739. (in Chinese)
Li Y, Sun R, Yu J, Saravanakumar K, Chen J. 2016. Antagonistic and biocontrol potential of Trichoderma asperellum ZJSX5003 against the maize stalk rot pathogen Fusarium graminearum. Indian Journal of Microbiology, 56, 318–327.
Liu H, Carvalhais L C, Kazan K, Schenk P M. 2016. Development of marker genes for jasmonic acid signaling in shoots and roots of wheat. Plant Signaling and Behavior, 11, e1176654.
Ma C, Zhai C X, Wang L A, Xia C, Li Y, Guo X, Cui S, Li G. 2006. Induced resistance by the toxin filtrate of Bipolaris maydis race T cultivation. Agricultural Sciences in China, 5, 678–684.
Malik V K, Singh M, Hooda K S, Yadav N K, Chauhan P K. 2018. Efficacy of newer molecules, bioagents and botanicals against maydis leaf blight and banded leaf and sheath blight of maize. The Plant Pathology Journal, 34, 121–125.
Martínez Medina A, Fernandez I, Lok G B, Pozo M J, Pieterse C M, Van Wees S. 2017. Shifting from priming of salicylic acid to jasmonic acid regulated defences by Trichoderma protects tomato against the root knot nematode Meloidogyne incognita. New Phytologist, 213, 1363–1377.
Orole O O, Adejumo T O. 2009. Activity of fungal endophytes against four maize wilt pathogens. African Journal of Microbiology Research, 3, 969–973.
Perazzolli M, Dagostin S, Ferrari A, Elad Y, Pertot I. 2008. Induction of systemic resistance against Plasmopara viticola in grapevine by Trichoderma harzianum T39 and benzothiadiazole. Biological Control, 47, 228–234.
Perazzolli M, Roatti B, Bozza E, Pertot I. 2011. Trichoderma harzianum T39 induces resistance against downy mildew by priming for defense without costs for grapevine. Biological Control, 58, 74–82.
Polidoros A N, Mylona P V, Scandalios J G. 2001. Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress. Transgenic Research, 10, 555–569.
Prochazkova D, Sairam R K, Srivastava G C, Singh D V. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Science, 161, 765–771.
Rosler J, Krekel F, Amrhein N, Schmid J. 1997. Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity. Plant Physiology, 113, 175–179.
Kumar S, Rani A, Jha M M. 2009. Potential of Trichoderma spp. as biocontrol agents against pathogens causing maydis leaf blight of maize. Journal of Biological Control, 23, 89–91.
Saravanakumar K, Li Y, Yu C, Wang Q, Wang M, Sun J, Gao J, Chen J. 2017. Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium stalk rot. Scientific Reports, 7, 1771.
Shoresh M, Harman G E, Mastouri F. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology, 48, 21–43.
Šrobárová A, Eged S. 2005. Trichoderma and sulphoethyl glucan reduce maize root rot infestation and fusaric acid content. Plant Soil and Environment, 51, 322–327.
Vinale F, Sivasithamparam K, Ghisalberti E L, Marra R, Woo S L, Lorito M. 2008. Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry, 40, 1–10.
Wang M, Ma J, Fan L, Fu K, Yu C, Gao J, Li Y, Chen J. 2015. Biological control of southern corn leaf blight by Trichoderma atroviride SG3403. Biocontrol Science and Technology, 25, 1133–1146.
Wang M, Wang S, Ma J, Yu C, Gao J, Chen J. 2017. Detection of Cochliobolus heterostrophus races in South China. Journal of Phytopathology, 165, 681–691.
Wilson P S, Ahvenniemi P M, Lehtonen M J, Kukkonen M, Rita H, Valkonen J P T. 2008. Biological and chemical control and their combined use to control different stages of the rhizoctonia disease complex on potato through the growing season. Annals of Applied Biology, 153, 307–320.
Wu Q, Zhang L, Xia H, Yu C, Dou K, Li Y, Chen J. 2017. Omics for understanding synergistic action of validamycin A and Trichoderma asperellum GDFS1009 against maize sheath blight pathogen. Scientific Reports, 7, 40140.
Wu X. 2007. Formulation study of 10% difenoconazole water dispersible granules. Anhui Chemical Industry, 33, 33–34. (in Chinese)
No related articles found!
No Suggested Reading articles found!