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Crosstalk of cold and gibberellin effects on bolting and flowering in flowering Chinese cabbage |
SONG Shi-wei, LEI Yu-ling, HUANG Xin-min, SU Wei, CHEN Ri-yuan, HAO Yan-wei |
College of Horticulture, South China Agricultural University, Guangzhou 510642, P.R.China |
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Abstract The flower stalk is the product organ of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee), which is cultivated extensively in South China. Flower stalk formation and development, including bolting and flowering, determine the yield of flowering Chinese cabbage; however, the bolting and flowering mechanisms remain to be explored. To elucidate these processes, we studied the effects of low-temperature and gibberellin (GA) treatments, and their interaction, on stem elongation, bolting time, flowering time, hormone content, and cell morphology in stem of flowering Chinese cabbage. The results showed that both cold and GA treatments accelerated bolting time, stem elongation, and flowering time. Moreover, cold and GA cotreated plants displayed additive positive effects. In addition, cold treatments increased the GA, indole-3-acetic acid, and cytokinin contents and altered cell size in the shoot apices of flowering Chinese cabbage. Treatment with uniconazole, a GA synthesis inhibitor, strongly delayed bolting time, stem elongation, and flowering time, whereas GA, but not cold treatment, rescued this inhibition, indicating that low temperature accelerates bolting and flowering not only through inducing GA in the shoot apices, but also other ways. These results provide a theoretical basis for further dissecting the regulatory mechanism of bolting and flowering in flowering Chinese cabbage.
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Received: 12 March 2018
Accepted:
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Fund: This work was supported by the earmarked fund for China Agriculture Research System (CARS-25-C-04) and the Natural Science Foundation of Guangdong, China (2016A030313399). |
Corresponding Authors:
Correspondence CHEN Ri-yuan, Tel: +86-20-38294595, E-mail: rychen@scau.edu.cn; HAO Yan-wei, Tel: +86-20-85280228, E-mail: yanweihao@scau.edu.cn
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About author: SONG Shi-wei, E-mail: swsong@scau.edu.cn; |
Cite this article:
SONG Shi-wei, LEI Yu-ling, HUANG Xin-min, SU Wei, CHEN Ri-yuan, HAO Yan-wei.
2019.
Crosstalk of cold and gibberellin effects on bolting and flowering in flowering Chinese cabbage. Journal of Integrative Agriculture, 18(5): 992-1000.
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Amasino M, Michaels D. 2010. The timing of flowering. Plant Physiology, 154, 516–520.
Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. 2003. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell, 115, 591–602.
Bunce A. 1985. Effects of day and night temperature and temperature variation on photosynthetic characteristics. Photosynthesis Research, 6, 175–181.
Dahanayake R, Galwey W. 1999. Effects of interactions between low-temperature treatments, gibberellin (GA3) and photoperiod on flowering and stem height of spring rape (Brassica napus var. annua). Annals of Botany, 84, 321–327.
Goldental-Cohen S, Israeli A, Ori N, Yasuor H. 2017. Auxin response dynamics during wild-type and entire flower development in tomato. Plant and Cell Physiology, 58, 1661–1672.
Hébrard C, Trap-Gentil V, Lafon-Placette C, Delaunay A, Joseph C, Lefèbvre M, Barnes S, Maury S. 2013. Identification of differentially methylated regions during vernalization revealed a role for RNA methyltransferases in bolting. Journal of Experimental Botany, 64, 651–663.
Huang X, Lei Y, Guan H, Hao Y, Liu H, Sun G, Chen R, Song S. 2017. Transcriptomic analysis of the regulation of stalk development in flowering Chinese cabbage (Brassica campestris) by RNA sequencing. Scientific Reports, 7, 1–14.
Hui M, Zhang L, Gong Z, Zhang M, Hou P. 2004. Effects of vernalization temperature on flower bud differentiation and bolting of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Acta Botanica Boreali-Occidentalia Sinica, 24, 2359-2361. (in Chinese)
Izumi K, Oshio H. 1991. Effects of the growth retardant uniconazole-P on endogenous levels of hormones in rice plants. In: Takahashi N, Phinney B O, MacMillan J, eds., Gibberellins. Springer, New York, NY. pp. 330–338.
de Jong M, Wolters-Arts M, García-Martínez L, Mariani C, Vriezen H. 2011. The Solanum lycopersicum AUXIN RESPONSE FACTOR 7 (SlARF7) mediates cross-talk between auxin and gibberellin signalling during tomato fruit set and development. Journal of Experimental Botany, 62, 617–626.
Jung C, Müller E. 2009. Flowering time control and applications in plant breeding. Trends in Plant Science, 14, 563–573.
Kang Y, Huang A, Yang X, Liu X. 2010. Effects of ABA and uniconazole on bolting characteristic and growth of flowering Chinese cabbage. Agricultural Science in Guangdong, 12, 49–51. (in Chinese)
Lambrechts H, Rook F, Kolloffel C. 1994. Carbohydrate status of tulip bulbs during cold-induced flower stalk elongation and flowering. Plant Physiology, 104, 515–520.
Lei L. 2016. Study on the bolting characteristics and the change of hormone of flowering Chinese cabbage under low temperature treatment. MSc thesis, South China Agricultural University, China. (in Chinese)
Li S. 2014. Influences of vernalization on bolting and blossom of radish. MSc thesis, Henan University of Science and Technology, China. (in Chinese)
Liang N, Cheng D, Liu Q, Cui J, Luo C. 2018. Difference of proteomics vernalization-induced in bolting and flowering transitions of Beta vulgaris. Plant Physiology and Biochemistry, 123, 222–232.
Liu S. 2010. Effects of temperature and photoperiod on spinach bolting. MSc thesis, Shandong Agricultural University, China. (in Chinese)
Mathieu S, Lutts S, Vandoorne B, Descamps C, Périlleux C, Dielen V, Van Herck C, Quinet M. 2014. High temperatures limit plant growth but hasten flowering in root chicory (Cichorium intybus) independently of vernalisation. Journal of Plant Physiology, 171, 109–118.
Nie S, Li C, Wang Y, Xu L, Muleke M, Tang M, Sun X, Liu L. 2016. Transcriptomic analysis identifies differentially expressed genes (DEGs) associated with bolting and flowering in radish (Raphanus sativus L.). Frontiers in Plant Science, 7, 682.
Olszewski N, Sun T P, Gubler F. 2002. Gibberellin signaling: Biosynthesis, catabolism, and response pathways. The Plant Cell, 14, S61–S80.
Pressman E, Shaked R. 1988. Bolting and flowering of Chinese cabbage as affected by the intensity and source of supplementary light. Scientia Horticulturae, 34, 177–181.
Ranwala P, Miller B. 2008. Gibberellin-mediated changes in carbohydrate metabolism during flower stalk elongation in tulips. Plant Growth Regulation, 55, 241–248.
Regnault T, Daviere M, Heintz D, Lange T, Achard P. 2014. The gibberellin biosynthetic genes AtKAO1 and AtKAO2 have overlapping roles throughout Arabidopsis development. The Plant Journal, 80, 462–474.
Ross J, Weston E, Davidson E, Reid B. 2011. Plant hormone interactions: How complex are they? Physiologia Plantarum, 141, 299–309.
Suge H, Rappaport L. 1968. Role of gibberellins in stem elongation and flowering in radish. Plant Physiology, 43, 1208–1214.
Sun H, Lei J. 2008. Study on control of flowering with gibberellin treatment in Clivia miniata Regel. Northern Horticulture, (4), 172–174. (in Chinese)
Sun Q, Yang Y, Lin D. 2008. Research progress on cruciferous vegetables bolting and flowering. In: Proceedings of 8th Youth Symposium of the Chinese Horticultural Society on the Modern Horticulture Researches. Shanghai Jiao Tong University Press, Shanghai. pp. 492–496. (in Chinese)
Tarkowská D, Filek M, Biesaga-Ko?cielniak J, Marcińska I, Machá?ková I, Krekule J, Strnad M. 2012. Cytokinins in shoot apices of Brassica napus plants during vernalization. Plant Science, 187, 105–112.
Wang L, Li M, Tian C, Hou L. 2009. Effects of gibberellins treatment on growth and development of cabbage. Journal of Shanxi Agricultural Sciences, 37, 58–60. (in Chinese)
Wang S, Xu W, He Q, Liu J, Zhang C. 2003. Effect of vernalization depth on bolting of radish and changes of GA3 and IAA contents during radish bolting. Shandong Agricultural Sciences, 9, 20–21. (in Chinese)
Xu X, van Lammeren A, Vermeer E, Vreugdenhil D. 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology, 117, 575–584.
Yan F, Hu G, Ren Z, Deng W, Li Z. 2015. Ectopic expression a tomato KNOX gene Tkn4 affects the formation and the differentiation of meristems and vasculature. Plant Molecular Biology, 89, 589–605.
Yan W, Hunt L A. 1999. Reanalysis of vernalization data of wheat and carrot. Annals of Botany, 84, 615–619.
Yang T, Davies P J, Reid J B. 1996. Genetic dissection of the relative roles of auxin and gibberellin in the regulation of stem elongation in intact light grown peas. Plant Physiology, 110, 1029–1034.
Zanewich P, Rood B. 1995. Vernalization and gibberellin physiology of winter canola. Plant Physiology, 108, 615–621.
Zhong L, Zhang Y, Liu H, Sun G, Chen R, Song S. 2016. Agrobacterium-mediated transient expression via root absorption in flowering Chinese cabbage. SpringerPlus, 5, 1825.
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