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Journal of Integrative Agriculture  2017, Vol. 16 Issue (03): 664-671    DOI: 10.1016/S2095-3119(16)61473-9
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw, local vegetable by-products and alfalfa in Southeast China
WANG Jian1, 2, CHEN Lei1, YUAN Xian-jun1, GUO Gang1, LI Jun-feng1, BAI Yun-feng3, SHAO Tao1

1 Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.China

2 College of Agriculture, Hainan University, Haikou 570228, P.R.China

3 Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China

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Abstract  This experiment was conducted to study the effect of molasses on the fermentation characteristics of mixed silage ensiled rice straw and vegetable by-products with alfalfa.  Mixture (202 g kg–1 dry matter (DM)) consisting of rice straw, broccoli residue and alfalfa at the ratio of 5:4:1 was ensiled with three experimental treatments: (1) no additives (control); (2) molasses at 2.5% (M1); (3) molasses at 5% (M2) on a fresh matter basis of mixture, respectively.  All treatments were packed into laboratory-scale silos, and three silos per treatment were sampled on days 1, 3, 5, 14 and 30.  The result showed that the pH value of all mixed silages decreased gradually with the time of ensiling except for the control silage, in which a significant increase (P<0.05) on day 30 occurred.  The lactic acid content increased gradually with the time of ensiling and reached the highest value on day 14, and a marked decrease (P<0.05) was found in the control silage on day 30.  The changes of acetic acid content showed similar pattern with lactic acid content.  A trace amount of propionic and butyric acid contents were found in the three mixed silages during the fermentation period.  Comparing to the control, M1 and M2 treatments improved the fermentation quality of mixed silages as indicated by higher (P<0.05) lactic acid contents and lower (P<0.05) pH and ammonia-N contents.  The Flieg points also showed that M1 and M2 silages were well preserved, whereas the control silage had a bad quality.  Overall, the findings of this study suggested that adding molasses could improve fermentation quality of mixed silage, and M1 was more suitable for practical application.
Keywords:  by-products      fermentation characteristics      mixed silage      molasses  
Received: 25 March 2016   Accepted:
Fund: 

This work was supported by the project of Jiangsu Independent Innovation, China (CX(15)1003-3), the Key Technologies R&D Program of China during the 13th Five Year Plan period (2016YFC0502005), and the special project of grass of Tibetan Autonomous Region for the “13th Five-Year” Plan, China.

Corresponding Authors:  SHAO Tao, Tel/Fax: +86-25-84396356, E-mail: taoshaolan@163.com    
About author:  WANG Jian, Mobile: +86-15261874002, E-mail: clsungodlwx@126.com

Cite this article: 

WANG Jian, CHEN Lei, YUAN Xian-jun, GUO Gang, LI Jun-feng, BAI Yun-feng, SHAO Tao . 2017. Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw, local vegetable by-products and alfalfa in Southeast China. Journal of Integrative Agriculture, 16(03): 664-671.

Adesogan A T, Salawu M B. 2002. The effect of different additives on the fermentation quality, aerobic stability and in vitro digestibility of pea/wheat bi-crop silages containing contrasting pea to wheat ratios. Grass and Forage Science, 57, 25–32.
Alli I, Fairbairn R, Noroozi E, Baker B E. 1984. The effects of molasses on the fermentation of chopped whole-plant leucaena. Journal of the Science of Food and Agriculture, 35, 285–289.
Amer S, Hassanat F, Berthiaume R, Seguin P, Mustaf A F. 2012. Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Animal Feed Science and Technology, 177, 23–29.
AOAC (Association of Official Analytical Chemists). 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Arlington.
Bampidis V A, Robinson P H. 2006. Citrus by-products as ruminant feeds: A review. Animal Feed Science and Technology, 128, 175–217.
Cao Y, Cai Y, Hirakubo T, Fukui H, Matsuyama H. 2011. Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons. Animal Science Journal, 82, 259–266.
Cao Y, Takahashi T, Horiguchi K I. 2009. Effects of addition of food by-products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Animal Feed Science and Technology, 151, 1–11.
Castle M E, Watson J N. 1985. Silage and milk production: Studies with molasses and formic acid as additives for grass silage. Grass and Forage Science, 40, 85–92.
Chen L, Guo G., Yuan X, Shimojo M, Yu C, Shao T. 2014. Effect of applying molasses and propionic acid on fermentation quality and aerobic stability of total mixed ration silage prepared with whole-plant corn in Tibet. Asian-Australasian Journal of Animal Sciences, 27, 349–356.
Dunière L, Sindou J, Chaucheyras-Durand F, Chevallier I, Thévenot-Sergentet D. 2013. Silage processing and strategies to prevent persistence of undesirable microorganisms. Animal Feed Science and Technology, 182, 1–15.
Guo G, Yuan X, Li L, Wen A, Shao T. 2014. Effects of fibrolytic enzymes, molasses and lactic acid bacteria on fermentation quality of mixed silage of corn and hulless-barely straw in the Tibetan Plateau. Grassland Science, 60, 240–246.
Kilic A. 1986. Silo Feed (Instruction, Education and Application Proposals). Bilgehan Press, Izmir, Turkey. p. 327.
Kung Jr L, Taylor C C, Lynch M P, Neylon J M. 2003. The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows. Journal of Dairy Science, 86, 336–343.
Lima R, Lourenço M, Díaz RF, castro A, Fievez V. 2010. Effect of combined ensiling of sorghum and soybean with or without molasses and lactobacilli on silage quality and in vitro rumen fermentation. Animal Feed Science and Technology, 155, 122–131.
McDonald P, Henderson A R, Heron S J E. 1991. The Biochemistry of Silage. 2nd ed. Chalcombe Publications, Bucks, UK. pp. 81–166.
Moselhy M A, Borba J P, Borba A E. 2015. Improving the nutritive value, in vitro digestibility and aerobic stability of Hedychium gardnerianum silage through application of additives at ensiling time. Animal Feed Science and Technology, 206, 8–18.
NRC (National Research Council). 2001. Nutrient Requirements of Dairy Cattle. 7th ed. The National Academies Press, Washington, D.C.
Pahlow G, Muck R E, Driehuis F, Oudeel Ferink S J W H. 2003. Microbiology of ensiling. In: Buxton D R, Muck R E, Harrison J H, eds., Silage Science and Technology. Agronomy Monograph No. 42. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, WI, USA. pp. 31–94.
Playne M J, McDonald P. 1966. The buffering constituents of herbage and silage. Journal of the Science of Food and Agriculture, 17, 264–266.
Porter M G, Murray R S. 2001. The volatility of components of grass silage on oven drying and the inter-relationship between dry-matter content estimated by different analytical methods. Grass and Forage Science, 56, 405–411.
SAS (Statistical Analysis System). 2001. User’s Guide: Statistics. ver. 8.2 edition. SAS Institute, Cary, NC.
Savoie P, Jofriet J C. 2003. Silage storage. In: Buxton D R, Muck R E, Harrison J H, eds., Silage Science and Technology. Agronomy Publication No. 42. American Society of Agronomy, Madison, WI, USA. pp. 405–467.
Shao T, Shimojo M, Wang T, Masuda Y. 2005. Effect of additives on the fermentation quality and residual mono- and di-saccharides compositions of forage oats (Avena sativa L.) and Italian ryegrass (Lolium multiflorum Lam.) silages. Asian-Australasian Journal of Animal Sciences, 18, 1582–1588.
Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597.
Thomas T A. 1977. An automated procedure for the determination of soluble carbohydrates in herbage. Journal of the Science of Food and Agriculture, 28, 639–642.
Weinberg Z G, Chen Y, Weinberg P. 2008. Ensiling olive cake with and without molasses for ruminant feeding. Bioresource Technology, 99, 1526–1529.
Wiedenhoeft M H, Barton B A. 1994. Management and environment effects on Brassica forage quality. Agronomy Journal, 86, 227–237.
Wuisman Y, Hiraoka H, Yahaya M S, Takeda M, Kim W, Takahashi T, Goto M. 2006. Effects of phenylalanine fermentation byproduct and sugarcane molasses on fermentation quality and rumen degradation of whole crop barley (Hordeum vulgare L.) silage in situ. Grassland Science, 52, 73–79.
Yi Y B, Ha M G, Lee J W, Park S M, Choi Y H, Chung C H. 2013. Direct conversion of citrus peel waste into hydroxymethylfurfural in ionic liquid by mediation of fluorinated metal catalysts. Journal of Industrial and Engineering Chemistry, 19, 523–528.
Yunus M, Ohba N, Shimojo M, Furuse M, Masuda Y. 2000. Effects of adding urea and molasses on napiergrass silage quality. Asian-Australasian Journal of Animal Sciences, 13, 1542–1547.
Zhang Y G, Xin H S, Hua J L. 2010. Effects of treating whole-plant or chopped rice straw silage with different levels of lactic acid bacteria on silage fermentation and nutritive value for lactating Holsteins. Asian-Australasian Journal of Animal Sciences, 23, 1601–1607.
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