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Journal of Integrative Agriculture  2016, Vol. 15 Issue (05): 1157-1165    DOI: 10.1016/S2095-3119(15)61274-6
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Differential volatile organic compounds in royal jelly associated with different nectar plants
ZHAO Ya-zhou1, 2, LI Zhi-guo2, TIAN Wen-li1, FANG Xiao-ming1, SU Song-kun2, PENG Wen-jun1
1 Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P.R.China
2 College of Bee Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
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Abstract      The aim of this work was to distinguish volatile organic compound (VOC) profiles of royal jelly (RJ) from different nectar plants.  Headspace solid-phase microextraction (HS-SPME) was used to extract VOCs from raw RJ harvested from 10 nectar plants in flowering seasons.  Qualitative and semi-quantitative analysis of VOCs extracts were performed by gas chromatography-mass spectrometry (GC-MS).  Results showed that VOC profiles of RJ from the samples were rich in acid, ester and aldehyde compound classes, however, contents of them were differential, exemplified by the data from acetic acid, benzoic acid methyl ester, hexanoic acid and octanoic acid.  As a conclusion, these four VOCs can be used for distinguishing RJ harvested in the seasons of different nectar plants.
Keywords:  royal jelly        volatile organic compound       nectar plant       headspace solid-phase microextraction       gas chromatography-mass spectrometry  
Received: 06 February 2015   Accepted:
Fund: 

This work was supported by the Agricultural Science and Technology Innovation Program, China (ASTIP) and the Building of Modern Agricultural Industry (Bees) R&D Systems in China (NYCYTI-43-KXJ17).

Corresponding Authors:  PENG Wen-jun, Tel/Tex: +86-10-62597059, E-mail: pengwenjun@vip.sina.com; SU Song-kun, Tel/Tex: +86-591-83739448, E-mail: susongkun@zju.edu.cn   
About author:  ZHAO Ya-zhou, E-mail: zhaoyazhou@caas.cn

Cite this article: 

ZHAO Ya-zhou, LI Zhi-guo, TIAN Wen-li, FANG Xiao-ming, SU Song-kun, PENG Wen-jun. 2016. Differential volatile organic compounds in royal jelly associated with different nectar plants. Journal of Integrative Agriculture, 15(05): 1157-1165.

Anklam E. 1998. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63, 549–562.

Anklam E, Radovic B. 2001. Suitable analytical methods for determining the origin of European honey. American Laboratory, 33, 60–62.

Baroni M V, Nores M L, Diaz M D P, Chiabrando G A, Fassano J P, Costa C, Wunderlin D A. 2006. Determination of volatile organic compound patterns characteristics of five unifloral honeys by solid-phase microextraction-gas chromatography-mass spectrometry coupled to chemimetrics. Journal of Agricultural and Food Chemistry, 54, 7235–7241.

Bertelli D, Papotti G, Lolli M, Sabatini A G, Plessi M. 2008. Development of an HS-SPME-GC method to determine the methyl anthranilate in Citrus honeys. Food Chemistry, 108, 297–303.

Boselli E, Caboni M F, Sabatini A G, Marcazzan G L, Lercker G. 2003. Determination and changes of free amino acids in royal jelly during storage. Apidologie, 34, 129–138.

Careri M, Mangia A, Barbieri G, Bouoni L, Virgili R, Parolari G. 1993. Sensory property relationships to chemical data of Italian-type dry-cured ham. Journal of Food Science, 58, 968–972.

Chen C, Chen S Y. 1995. Changes in protein components and storage stability of royal jelly under various conditions. Food Chemistry, 54, 195–200.

Daniele G, Casabianca H. 2012. Sugar composition of French royal jelly for comparison with commercial and artificial sugar samples. Food Chemistry, 134, 1025–1029.

Ferioli F, Armaforte E, Caboni M F. 2014. Comparison of the lipid content, fatty acid profile and sterol composition in local Italian and commercial royal jelly samples. Journal of the American Oil Chemists’ Society, 91, 875–884.

Ferrer E, Alegr?a A, Farre R, Abellan P, Romero F. 2002. High-performance liquid chromatographic determination of furfural compounds in infant formulas: Changes during heat treatment and storage. Journal of Chromatography A, 947, 85–95.

Isidorov V A, Bakier S, Grzech I. 2012. Gas chromatographic-mass spectrometric investigation of volatile and extractable compounds of crude royal jelly. Journal of Chromatography B, 885, 109–116.

Isidorov V A, Czy?ewska U, Isidorova A G, Bakier S. 2009. Gas chromatographic and mass spectrometric characterization of the organic acids extracted from some preparations containing lyophilized royal jelly. Journal of Chromatography B, 877, 3776–3780.

Isidorov V A, Czy?ewska U, Jankowska E, Bakier S. 2011. Determination of royal jelly acids in honey. Food Chemistry, 124, 387–391.

Jalali-Heravi M, Parastar H. 2011. Recent trends in application of multivariate curve resolution approaches for improving gas chromatography-mass spectrometry analysis of essential oils. Talanta, 85, 835–849.

Kaškonien? V, Venskutonis P R, ?eksteryt? V. 2008. Composition of volatile compounds of honey of various floral origin and beebread collected in Lithuania. Food Chemistry, 111, 988–997.

Li J, Wang T, Zhang Z, Pan Y. 2007. Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera). Journal of Agricultural and Food Chemistry, 55, 8411–8422.

Liu Y, Lotero E, Goodwin Jr J G. 2006. Effect of water on sulfuric acid catalyzed esterification. Journal of Molecular Catalysis (A: Chemical), 245, 132–140.

Manyi-Loh C E, Ndip R N, Clarke A M. 2011. Volatile compounds in honey: A review on their involvement in aroma, botanical origin determination and potential biomedical activities. International Journal of Molecular Sciences, 12, 9514–9532.

Moazeni-Pourasil R S, Piri F, Ghassempour A, Jalali-Heravi M. 2014. The use of multivariate curve resolution methods to improve the analysis of muramic acid as bacterial marker using gas chromatography-mass spectrometry: An alternative method to gas chromatography-tandem mass spectrometry. Journal of Chromatography B, 949, 1–6.

Overton, S V, Manura J J. 1995. Flavor and aroma in natural bee honey. [2015-1-10] http://www.sisweb.com/referenc/applnote/app-25.htm

Ponnusamy V K, Jen V F. 2011. A novel graphene nanosheets coated stainless steel fiber for microwave assisted headspace solid phase microextraction of organochlorine pesticides in aqueous samples followed by gas chromatography with electron capture detection. Journal of Chromatography A, 1218, 6861–6868.

Ramadan M F, Al-Ghamdi A. 2012. Bioactive compounds and health-promoting properties of royal jelly: A review. Journal of Functional Foods, 4, 39–52.

Sabatini A G, Marcazzan G L, Caboni M F, Bogdanov S, Almeida-Muradian L. 2009. Quality and standardisation of royal jelly. Journal of ApiProduct and ApiMedical Science, 1, 1–6.

Sereia M J, Toledo V D A A D, Furlan A C, Faquinello P, Maia F M C, Wielewski P. 2013. Alternative sources of supplements for Africanized honeybees submitted to royal jelly production. Acta Scientiarum Animal Sciences, 35, 165–171.

Sesta G, Lusco L. 2008. Refractometric determination of water content in royal jelly. Apidologie, 39, 225–232.

Snow N H, Bullock G P. 2010. Novel techniques for enhancing sensitivity in static headspace extraction-gas chromatography. Journal of Chromatography A, 1217, 2726–2735.

Stocker A, Schramel P, Kettrup A, Bengsch E. 2005. Trace and mineral elements in royal jelly and homeostatic effects. Journal of Trace Elements in Medicine and Biology, 19, 183–189.

Vázquez L C, Díaz-Maroto M C, Guchu E, Perez-Coéllo M S. 2006. Analysis of volatile compounds of eucalyptus honey by solid phase extraction followed by gas chromatography coupled to mass spectrometry. European Food Research and Technology, 224, 27–31.

Wolski T, Tambor K, Rybak-Chmielewska H, Kedzia B. 2006. Identification of honey volatile components by solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS). Journal of Apicultural Science, 50, 115–126.

Wu L M, Zhou J H, Xue X F, Li Y, Zhao J. 2009. Fast determination of 26 amino acids and their content changes in royal jelly during storage using ultra-performance liquid chromatography. Journal of Food Composition and Analysis, 22, 242–249.

Zhou J, Xue X, Chen F, Zhang J, Li Y, Wu L, Chen L, Zhao J. 2009. Simultaneous determination of seven fluoroquinolones in royal jelly by ultrasonic-assisted extraction and liquid chromatography with fluorescence detection. Journal of Separation Science, 32, 955–964.

Zhou L, Xue X, Zhou J, Li Y, Zhao J, Wu L. 2012. Fast determination of adenosine 5′-triphosphate (ATP) and its aatabolites in royal jelly using ultraperformance liquid chromatography. Journal of Agricultural and Food Chemistry, 60, 8994–8999.
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