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Journal of Integrative Agriculture  2016, Vol. 15 Issue (8): 1833-1845    DOI: 10.1016/S2095-3119(15)61186-8
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
The cellular interactome for glycoprotein 5 of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus
DU Ji-ge*, GE Xin-na*, DONG Hong, ZHANG Ning, ZHOU Lei, GUO Xin, YANG Han-chun
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China
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Abstract     The glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) is a multi-functional protein that plays important roles in virus assembly, entry and viral anti-host responses. In the present study, we investigated the cellular binding partners of GP5 by using lentivirus transduction coupled with immunoprecipitation and mass spectrometry. There were about 40 cellular proteins identified with high Confidence Icons by MS/MS. Ingenuity Pathway Analysis (IPA) indicated that these proteins could be assigned to different functional classes and networks. Furthermore, we validated some of the interactions by co-immunoprecipitation (Co-IP) and confocal microscopy, including those with mitofilin, a mitochondrial inner membrane protein that might be involved in PRRSV or GP5-induced apoptosis, and calnexin, a protein chaperone that might facilitate the folding and maturation of GP5. The interactome data contribute to understand the role and molecular mechanisms of GP5 in PRRSV pathogenesis.
Keywords:  PRRSV        glycoprotein 5 (GP5)        interactome profile        mitofilin        calnexin  
Received: 28 August 2015   Accepted:
Fund: 

This study was supported by the Earmarked Fund for Modern Agro-industry Technology Research System of China (CARS-36) from the Ministry of Agriculture of China, the National Natural Science Foundation of China (31572549) and the National Key Technology R&D Program of China (2015BAD12B01-2) from the Ministry of Science and Technology of China.

Corresponding Authors:  GUO Xin, Tel/Fax: +86-10-62731296, E-mail: guoxin@cau.edu.cn; YANG Han-chun, Tel/Fax: +86-10-62731296, E-mail: yanghanchun1@cau.edu.cn   
About author:  DU Ji-ge, Tel: +86-10-62733962, E-mail: du19371@163.com; GE Xin-na, Tel: +86-10-62733962, E-mail: gexinna@126.com;

Cite this article: 

DU Ji-ge, GE Xin-na, DONG Hong, ZHANG Ning, ZHOU Lei, GUO Xin, YANG Han-chun. 2016. The cellular interactome for glycoprotein 5 of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus. Journal of Integrative Agriculture, 15(8): 1833-1845.

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