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| Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations |
| ZHAO Xia, JIA Miao, WANG Lei, CAO Guang-chun , ZHANG Ze-hua |
1、State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China
2、College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China |
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摘要 Rapid and mass rearing of Locusta migratoria manilensis is an urgent need to meet the increasing demand for food of people. In this study, the effects of four artificial feeds on the development, reproduction and the activities of detoxification and protective enzymes of L. migratoria manilensis in three successive generations were investigated. The results showed that sucrose and monosodium glutamate (MSG) significantly increased the net reproductive rate (R0) and the intrinsic growth rate (rm) of L. migratoria manilensis, but sodium chloride (0.17%) suppressed this increase. Furthermore, the artificial feed with sucrose and monosodium glutamate increased the activities of esterase (EST), acetylcholinesterase (AChE), glutathione-Stransferase (GST), multi-function oxidase (MFO), phenol oxidase (PO), catalase (CAT) and peroxidase (POD), but inhibited the activity of superoxide dismutase (SOD). However, sodium chloride (0.17%) increased the activities of EST, AChE, CAT and SOD, and inhibited the activities of MFO, GST, PO and POD. Correlation analysis found that the increasing of PO activity and the decreasing of SOD activities were significantly related with the increasing of the intrinsic growth rate (rm). The above results indicated that sucrose and monosodium glutamate could promote the development and reproduction of L. migratoria manilensis, but Na+ inhibit such promotion with the concentration above 0.2%. The activities of PO and SOD can be used as biochemical standard to assess the effect of artificial feed.
Abstract Rapid and mass rearing of Locusta migratoria manilensis is an urgent need to meet the increasing demand for food of people. In this study, the effects of four artificial feeds on the development, reproduction and the activities of detoxification and protective enzymes of L. migratoria manilensis in three successive generations were investigated. The results showed that sucrose and monosodium glutamate (MSG) significantly increased the net reproductive rate (R0) and the intrinsic growth rate (rm) of L. migratoria manilensis, but sodium chloride (0.17%) suppressed this increase. Furthermore, the artificial feed with sucrose and monosodium glutamate increased the activities of esterase (EST), acetylcholinesterase (AChE), glutathione-Stransferase (GST), multi-function oxidase (MFO), phenol oxidase (PO), catalase (CAT) and peroxidase (POD), but inhibited the activity of superoxide dismutase (SOD). However, sodium chloride (0.17%) increased the activities of EST, AChE, CAT and SOD, and inhibited the activities of MFO, GST, PO and POD. Correlation analysis found that the increasing of PO activity and the decreasing of SOD activities were significantly related with the increasing of the intrinsic growth rate (rm). The above results indicated that sucrose and monosodium glutamate could promote the development and reproduction of L. migratoria manilensis, but Na+ inhibit such promotion with the concentration above 0.2%. The activities of PO and SOD can be used as biochemical standard to assess the effect of artificial feed.
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Received: 27 February 2013
Accepted:
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| Fund: This study was supported by the the Special Fund for Agro-Scientific Research in the Public Interest of China (201003079) and the Earmarked Fund for China Agriculture Research System (CARS-35-07). |
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Corresponding Authors:
CAO Guang-chun, Tel/Fax: +86-10-82109585, E-mail: caoguangchun77@163.com
E-mail: caoguangchun77@163.com
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Cite this article:
ZHAO Xia, JIA Miao, WANG Lei, CAO Guang-chun , ZHANG Ze-hua.
2014.
Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations. Journal of Integrative Agriculture, 13(4): 819-826.
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| Bradford M M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein binding. Analytical Biochemistry, 72, 248-254 Cai Q N, Zhang Q W, Cheo M. 2004. Contribution of indole alkaloids to Sitobion avenae (F.) resistance in wheat. Journal of Applied Entomology, 128, 517-521Cao C Q, Cheng S Z, Zhang Z J 2008. Effect of the artificial feed with different content of water on the growth and development of Locusta migratoria manilensis (Meyen). Journal of Anhui Agriculture Science, 36, 15920-15921, 15924 (in Chinese) Carpenter J E, Bloem S. 2003. Performance of natural enemies reared on artificial feeds. In: Proceedings of the International Symposium on Biological Control of Arthropods. Honolulu, Hawaii, USA. pp. 143-149 Chen Y L. 2000. Characteristics causes of formation and ecological management of locust plagues. Bulletin of Biology, 35, 1-5 (in Chinese) Cui X Y. 2010. Special breeding: Locusts. Popular Science & Technology, 4, 130-131 (in Chinese) Deryck P W. 1997. Laboratory rearing of the fall armyworm. The Florida Entomologist, 62, 87-91 Fang J, Zhu L, Yang Z D, Zhao B G. 2003. Advances and problems in insect artificial feed studies. Journal of Sichuan Forestry Science and Technology, 24, 18-26. (in Chinese) Guo J Y, Wu G, Wan F H. 2010. Activities of digestive and detoxification enzymes in multiple generations of beet armyworm, Spodoptera exigua (Hübner), in response to transgenic Bt cotton. Journal of Pest Science, 83, 453- 460. Han Z J, Moores G, Devonshire A, Denholm L. 1998. Association between biochemical marks and insecticide resistance in the cotton aphid, Aphis gossypii. Pesticide Biochemistry and Physiology, 62, 164-171 Hansen L G, Hodgson E. 1971. Biochemical characteristics of insect microsomes N- and O-demethylation. Biochemical Pharmacology, 20, 1569-1573 Hamamura Y, Kuwata K, Masada H. 1966. Effect of gallic acid on the growth of the silkworm nymph Bombyx morill. Nature, 212, 1386-1387 Howden P, Hunter J. 1958. An artificial feed for the laboratory rearing of locusts. Nature, 182, 1527. Hulting F L, Orr D B, Obrycki J J. 1990. A computer program for calculation and statistical comparison of intrinsic rate of increase and associated life parameters. Florida Entomologist, 73, 601-612 Isaac G S, Alex CA. 2011. Phenoloxidase: A key component of the insect immune system. Entomologia Experimentalis et Applicata, 10, 1570- 7458. Luo W C, Xue C B. 2010. Insects Phenol Oxidase and Inhibitor. Science Press, Beijing. p. 30. (in Chinese) Ma E B, He Y P, Zhu Y K. 2004. Comparative studies of acetylcholinesterases purified from two field populations of the oriental migratory locust (Locusta migratoria manilensis): Implications of insecticide resistance. Pesticide Biochemistry and Physiology, 78, 67-77 Oppenoort F J, Welling W. 1976. Bioehemistry and physiology of resistance. In: Insecticide Biochemistry and Physiology. Plenum Press, New York. pp. 507-551 Pang B P, Zhou X R, Shi L, Mu H B. 2004. Performance of Tetranychus truncatus Ehara (Acarina: Tetranychidae) reared with different host plants. Acta Entomologica Sinica, 47, 55-58 (in Chinese) Payne J A, Lowman H, Pate R R. 1975. Artificial feeds for rearing the tilehorned Prionus. Annals of the Entomological Society of America, 68, 680-682 SAS Institute. 1996. SAS/STAT Software: Changes and Enhancements Through Release 6.12. SAS Institute, Cary, NC Son Y Q, Sun H Z, Li T, Wu Z. 2009. Effect of the host plants on activity of 4 detoxifying enzymes in beet armyworm, Spodoptera exigua. Chinese Agricultural Science Bulletin, 25, 203-205 (in Chinese) Wu F Z. 1951. Locusts of China. Yongxiang Press, Shanghai. pp. 17-19. (in Chinese) Wu G, Chen F J, Ge F. 2006. Response of multiple generations of cotton bollworm Helicoverpa armigera Hübner, feeding on spring wheat, to elevated CO2. Journal of Applied Entomology, 130, 2-9 Wu G, Chen F J, Xiao N W, Ge F. 2010. Plant allocation to defensive compounds of transgenic Bt cotton in response to infestation by cotton bollworm under conditions of elevated CO2. International Journal of Pest Management, 56, 81-89 Wu G, Marvin K H, Guo J Y, Wan F H. 2009. Temporal allocation of metabolic tolerance in the body of beet armyworm in response to three gossypol-cotton cultivars. Science in China (Series C: Life Science), 52, 1140-1147 Wu K J, Gong P Y, Li X Z. 1990. The study on artificial feed of Heliothis assulta. Acta Entomologica Sinica, 33, 301-308. (in Chinese) Zhang B, Liu H, Helen H S, Wang J J. 2011. Effect of host plants on development, fecundity and enzyme activity of spodoptera exigua (Hübner) (Lepidoptera: Noctuidae). Agricultural Sciences in China, 10, 1232-1240. |
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