|
|
|
|
|
| Biological Effects of Low Energy N+ Beams Implantation on Calluses of Autotetraploid Rice |
| ZHAO Shuai-peng, HUANG Qun-ce, LIANG Qiu-xia, ZHANG Shu-gen, JIAO Zhen |
1.Province Key Laboratory of Ion Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou 450052,P.R.China
2.Qingdao Yuance Biological Technology Co. Ltd., Qingdao 266071, P.R.China |
|
|
|
摘要 Calluses of two autotetraploid rice (DPR (4) and Zijing (4)) were implanted with 30 KeV N+ beams at doses ranging from 1.0×1015 to 9.0×1015 ions cm-2. The differentiation rates of calluses, the concentration of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and the change of esterase (EST) spectrum were measured and analyzed on the calluses stage. The results showed that differentiation rates of calluses under 3.0×1015 ions cm-2 ((46.12±2.01)%) were higher than the other treatment groups in DPR (4), and those of Zijing (4) were similar under the doses of 1.0×1015, 3.0×1015 and 5.0×1015 ions cm-2 ((40.55±2.74), (37.77±3.04) and (34.56±2.65)%) and were higher than that of 7.0×1015 and 9.0×1015 ions cm-2. When the doses were 3.0×1015 and 5.0×1015 ions cm-2, the activities of SOD, POD and CAT were notably enhanced, and the accumulation of MDA content was markedly alleviated in the implanted calluses of two materials, as well as the activity of EST was increased by the additional isforms DPR (4). Whereas the anti-oxidative systems and enzymes spectrum of EST were destroyed seriously, the accumulation of MDA was significantly aggravated, also the differentiation rate of calluses reduced sharply and almost closed to zero when the implantation doses were 7.0×1015 and 9.0×1015 ions cm-2. It suggested that the proper dose of ion implantation effectively maintained the normal membrane structure and metabolism, and it would be also a feasible approach for autotetraploid rice to improve its genetic characteristics at calluses stage. Additionally, the differences of the radiation resistance between DPR (4) and Zijing (4) could be related to the different genotypes.
Abstract Calluses of two autotetraploid rice (DPR (4) and Zijing (4)) were implanted with 30 KeV N+ beams at doses ranging from 1.0×1015 to 9.0×1015 ions cm-2. The differentiation rates of calluses, the concentration of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and the change of esterase (EST) spectrum were measured and analyzed on the calluses stage. The results showed that differentiation rates of calluses under 3.0×1015 ions cm-2 ((46.12±2.01)%) were higher than the other treatment groups in DPR (4), and those of Zijing (4) were similar under the doses of 1.0×1015, 3.0×1015 and 5.0×1015 ions cm-2 ((40.55±2.74), (37.77±3.04) and (34.56±2.65)%) and were higher than that of 7.0×1015 and 9.0×1015 ions cm-2. When the doses were 3.0×1015 and 5.0×1015 ions cm-2, the activities of SOD, POD and CAT were notably enhanced, and the accumulation of MDA content was markedly alleviated in the implanted calluses of two materials, as well as the activity of EST was increased by the additional isforms DPR (4). Whereas the anti-oxidative systems and enzymes spectrum of EST were destroyed seriously, the accumulation of MDA was significantly aggravated, also the differentiation rate of calluses reduced sharply and almost closed to zero when the implantation doses were 7.0×1015 and 9.0×1015 ions cm-2. It suggested that the proper dose of ion implantation effectively maintained the normal membrane structure and metabolism, and it would be also a feasible approach for autotetraploid rice to improve its genetic characteristics at calluses stage. Additionally, the differences of the radiation resistance between DPR (4) and Zijing (4) could be related to the different genotypes.
|
|
Received: 18 October 2012
Accepted:
|
| Fund: This work was financially supported by the National Natural Science Foundation of China (10505018) and the Natural Science Foundation of Henan Province, China (091100110401). |
|
Corresponding Authors:
Correspondence HUANG Qun-ce, Tel: +86-371-67766836, E-mail: quncehuang@zzu.edu.cn
E-mail: quncehuang@zzu.edu.cn
|
| About author: ZHAO Shuai-peng, Mobile: 13721419852, E-mail: zhaoshuaipeng@163.com; |
Cite this article:
ZHAO Shuai-peng, HUANG Qun-ce, LIANG Qiu-xia, ZHANG Shu-gen, JIAO Zhen.
2013.
Biological Effects of Low Energy N+ Beams Implantation on Calluses of Autotetraploid Rice. Journal of Integrative Agriculture, 12(11): 2045-2055.
|
| [1]A-H-Mackerness S, Surplus S L, Jordan B R, Thomas B.1998. Effects of supplementary ultraviolet-B radiationon photosynthetic transcripts at different stages of leafdevelopment and light levels in pea (Pisum sativum L.):role of active oxygen species and antioxidant enzymes.Journal of Photochemistry and Photobiology, 68, 88-96[2]Antar N E, Ismael A K. 2012. Biochemical characterationof rice somaclones resistant to blast. Current ResearchJournal of Biological Sciences, 4, 137-142[3]Bailly C, Benamar A, Corbineau F. 1996. Changes inmalondialdehyde content and in superoxide dismutase,catalase and glutathione reductase activities in sunflowerseeds as related to deterioration during accelerated aging.Physiology Plant, 97, 104-110[4]Feng Y R, Chen Q F, Qin G Y, Ya H Y, Shang S P, GuL B, Ni K K. 2012. Semi-quantitative analysis of thefive up-regulated genes in rice screened by gentchiparray underlying the implantation of the low-energy ionbeams. Journal of Radition Research and RadiationProcess, 30, 48-52[5](in Chinese)Fu Q S, Zhao B, Wang X N, Wang Y J, Ren S X, GuoY D. 2011. The responses of morphological trait,leaf ultrastructure, photosynthetic and biochemicalperformance of tomao to differential light availabilities.Agricultural Sciences in China, 10, 1887-1897[6]Giannopolitis C N, Ries S K. 1977. Superxide dismutase: I.Occurrence in higher plants. Plant Physiology, 59, 309-314[7]Gu Y H, Gao F, Qi Y L, Jiao Z. 2011. Study on variationeffects caused by ion beam-mediated transformationwhose transformation receptors are wheat’s segregetionpopulation seeds. Life Sicence Journal, 8, 356-362[8]Han R, Wang X L, Yue M. 2002. Influence of He-Nelaser irradiation on the excision repair of cyclobutylpyrimidine dimers in the rice DNA. Chinese ScienceBulletin, 47, 818-821[9]He J H, Shahid M Q, Chen Z X, Chen X A, Liu X D, Lu Y G.2011. Abnormal PMC microtubule distribution patternand chromosome behavior resulted in low pollen fertilityof an intersubspecific autotetraploid rice hybrid. PlantSystematics and Evolution, 291, 257-265[10]He Z, He C, Zhang Z, Zou Z, Wang H. 2007. Changes ofantioxidative enzymes and cell membrane osmosis intomato colonized by arbuscular mycorrhizae underNaCl stress. Journal of Colloids and Surfaces (BBiointerfaces), 59, 128-133[11]Hodges D M, DeLong J M, Forney C F, Prange R K. 1999.Improving the thiobarbituric acid-reactive-substancesassay for estimating lipid peroxidation in plant tissuescontaining anthocyanin and other interfering compounds.Planta, 207, 604-611[12]Ji L, Li Y M, Wang C S, Cao G Q, Jia X. 2005. Studies on wheat mutants induced by nitrogen ion beamimplantation. Acta Genetica Sinica, 32, 1176-1183[13]Joshi P N, Biswal B, Biswal U C. 1991. Effect of UV-Aon aging of wheat leaves and role of phytochrome.Environmental and Experimental Botany, 31, 267-276[14]Khush G S. 2003. Productivity improvement in rice.Nutrition Reviews, 61, 114-116[15]Kikuchi S, Saito Y, Ryuto H, Fukunishi N, Abe T, TanakaH, Tsujimoto H. 2009. Effects of heavy-ion beamson chromosomes of common rice, Triticum aestivum.Journal of Mutation Research, 669, 63-66[16]Kozi A. 1992. Ascorbate peroxidase - a hydrogen peroxidescavengingenzyme in plants. Journal of PhysiologiaPlantarum, 85, 235-241[17]Kwon O S, Lee H. 2004. Productivity improvement inKorean rice farming: parametric and non-parametricanalysis. Australian Journal of Agricultural andResource Economics, 48, 323-346[18]Larkin P J, Scowcroft W P. 1981. Somaclonal variationanovel source of variability from cell culture for plantimprovements. Theoretical and Applied Genetics, 60,197-214[19]Latif M A, Omar M Y, Tan S G, Siraj S S, Ismail A R. 2010.Biochemical studies on malathion resistance, inheritanceand association of carboxylesterase activity in brownplanthopper, Nilaparvata lugens complex in Peninsularmalaysia. Insect Science, 17, 517-526[20]Li G P, Huang Q C, Qin G Y, Huo Y P. 2005. The effectsof low energy nitrogen ion implantation on pollen exinesubstructure and pollen germination of cedrus deodara.Plasma Science & Echnology, 7, 3176-3180[21]Li P R, Yan S L, Zhang D H, Li Z Y, Qin G Y, Huo Y P.2008. Mutagenic effects of bioflocculant-producingstrains by N+ ion Implantation. Journal of NuclearTechniques, 31, 193-196[22]Li S S, Zhang Y L. 1990. Studies on tissue culture of wheatimmature inflorescences and the differentiation of call.Journal of Wuhan Botanical Research, 8, 349-354[23]Li Y L, Tang J H, Qin GY, Huo Y P. 2008. Maize pollenmutation by low energy ion beams. The PhilippineAgriculture Scientist, 94, 395-400[24]Liang Q X, Huang Q C, Cao G Q. 2008. Study of biologicaleffect of low energy ion implantation on tomato andradish breeding. Plasma Science and Technology, 10,254-259[25]Luan L, Tu S B, Long W B, Wang X, Liu Y H, Kong F L,He T, Yan W G, Yu M Q. 2007. Cytogenetic studies ontwo F1 hybrids of autotetraploid rice varieties showingextremely high level of heterosis. Plant Systematics andEvolution, 267, 205-213[26]Luan L, Wang X, Long W B, Liu Y H, Tu S B, Zhao Z P,Kong F L, Yu M Q. 2008. Microsatellite analysis ofgenetic variation and population genetic differentiationin autotetraploid and diploid rice. Biochemical Genetics,46, 248-266[27]Murashige T, Skoog F. 1962. A revised medium for rapidgrowth and bioassay with tobacco tissue cultures.Physiologia Plantarum, 15, 473-497[28]Murphy T M. 1990. Effect of broad-band ultraviolet andvisible radiation on hydrogen peroxide formation bycultured rose cells. Physiology Plant, 80, 63-68[29]Nanthakumar M, Lakshmi V J, Bhushan V S, BalachandranS M, Mohan M. 2012. Decrease of rice plant resistanceand induction of hormesis and carboxylesterase titrein brown planthopper, Nilaparvata lugens (Stål) byxenobiotics. Pesticide Biochemistry and Physiology,102, 146-152[30]Normile D. 2008. Reinventing rice to feed the world.Science, 321, 330-333[31]Polle A, Otter T, Seifert F. 1994. Apoplastic peroxidesesand lignification in needles of norway sprue. PlantPhysiology, 106, 53-60[32]Qi Z, Yue M, Wang X. 2000. Laser pretreatment protectscells of broad bean from UV-B radiation damage.Journal of Photochemistry and Photobiology (B:Biology), 59, 33-37[33]Singh D, Mehta R, Talati J G. 2009. Identification of rice(Oryza sativa L.) varieties through biochemical markers.Indian Journal of Agriculture Biochemistry, 22, 18-25[34]Singh R, Singh S, Tripathi R, Agrawal S B. 2011.Supplemental UV-B radiation induced changes ingrowth, pigments and antioxidant pool of bean (Dolichoslablab) under field conditions. Journal of EnvironmentalBiology, 32, 139-145[35]Stapleton A E. 1992. Ultraviolet radiation and plants:burning questions. The Plant Cell, 4, 1353-1358[36]Torabi S, Niknam V. 2011. Effects of iso-osmoticconcentrations of NaCl and Mannitol on some metabolicactivity in calluseses of two salicornia species. In VitroCellular & Developmental Biology-Plant, 47, 734-742[37]Willekens H, Chamnongpol S, Davey M, Schraudner M,Langebartels C, Montagu M, Inzé D, Camp W. 1997.Catalase is a sink for H2O2 and is indispensable for stressdefense in C3 plants. The EMBO Journal, 16, 4806-4816[38]Yang C P, Shen Z Q, Yu G C, Wang J L. 2009. Effectof gamma ray radiation pretreatment on enzymatichydrolysis of wheat straw to produce sugar. AtomicEnergy Science and Technology, 43, 36-40[39](in Chinese)Yang P M, Huang Q C, Qin G Y, Zhao S P. 2011.Preliminary studies on some traits of autotetraploid rice.Southwest China Journal of Agricultural Science, 24,396-399[40](in Chinese)Yu Z L, Deng J, He J, Huo Y, Wu Y, Wang X, Lui G.1991. Mutation breeding by ion implantation. NuclearInstruments and Methods in Physics Research Section(B: Beam Interactions with Materials and Atoms), 59/60,705-708[41]Yu Z L. 2000. Ion beam application in genetic modification.IEEE Transactions on Plasma Science, 28, 128-132[42]Zhang G F, Shi X M, Nie Y L, Li K, Zhou H Y, LuT. 2005. The comparative research on effects of low energy N+ implantation and gama-radiation onsoluble proteins, activity of enzymes and isoamylasein Arabidopsis thaliana. Chinese High TechnologyLetters, 15, 112-118[43]Zhang J, Kirkham M B. 1996. Enzymatic responses of theascorbate-glutathione cycle to drought in sorghum andsunflower plants. Plant Science, 113, 139-147[44]Zhao S P, Huang Q C, Huang Y Q, Jia H R, Qin G Y. 2010.Biological effects of calluses formations in rice inducedby N+ implantations. Journal of Zhengzhou University(Natural Science Edition), 42, 115-119[45](in Chinese)Zhao S P, Huang Q C, Liang Q X, Chen X N. 2011.Physiological response of japonica rice to differenttime gradient treatment of colchicines. In: Proceedingsof Remote Sensing, Environment and TransportationEngineering (RSETE), 2011 International Conference.Nanjing, China. pp. 6982-6985[46]Zhou Z S, Huang S Q, Guo K, Mehta S K, Zhang P C, YangZ M. 2007. Metabolic adaptations to mercury-inducedoxidative stress in roots of Medicago sativa L. Journalof Inorganic Biochemistry, 101, 1-9 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
