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| Phenolic Profiles and Antioxidant Activity of Buckwheat (Fagopyrum esculentum Möench and Fagopyrum tartaricum L. Gaerth) Hulls, Brans and Flours |
| LI Fu-hua, YUAN Ya, YANG Xiao-lan, TAO Shu-ying , MING Jian |
1.College of Food Science, Southwest University, Chongqing 400715, P.R.China
2.Key Laboratory of Food Processing & Technology of Chongqing, Chongqing 400715, P.R.China
3.Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Presevation,, Mimnistry of Agriculture, Chongqing 400715, P.R.China |
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摘要 The extracts from hulls, brans and flours of Fagopyrum esculentum Möench (FEM, three varieties) and Fagopyrum tartaricum L. Gaerth (FTG, seven varieties) were screened for free and bound phenolic content or total phenolic content (TPC), as well as 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average EC50 84.54 μg mL-1 and IC50 11.54 μg mL-1 respectively, FTG brans extract had the highest average TPC (24.87 mg GAE g-1 DW), and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient (rs) between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively (P<0.05). However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
Abstract The extracts from hulls, brans and flours of Fagopyrum esculentum Möench (FEM, three varieties) and Fagopyrum tartaricum L. Gaerth (FTG, seven varieties) were screened for free and bound phenolic content or total phenolic content (TPC), as well as 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average EC50 84.54 μg mL-1 and IC50 11.54 μg mL-1 respectively, FTG brans extract had the highest average TPC (24.87 mg GAE g-1 DW), and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient (rs) between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively (P<0.05). However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
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Received: 29 October 2012
Accepted:
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| Fund: This work was supported by the Fundamental Research Funds for the Central Universities, China (XDJK2012B014). |
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Corresponding Authors:
Correspondence MING Jian, Tel: +86-23-68251298, Fax: +86-23-68251947, E-mail: mingjian1972@163.com
E-mail: mingjian1972@163.com
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| About author: LI Fu-hua, E-mail: fuhualee92@163.com |
Cite this article:
LI Fu-hua, YUAN Ya, YANG Xiao-lan, TAO Shu-ying , MING Jian.
2013.
Phenolic Profiles and Antioxidant Activity of Buckwheat (Fagopyrum esculentum Möench and Fagopyrum tartaricum L. Gaerth) Hulls, Brans and Flours. Journal of Integrative Agriculture, 12(9): 1684-1693.
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| [1]Adom K K, Liu R H. 2002. Antioxidant activity of grains.Journal of Agriculture and Food Chemistry, 50, 6182-6187[2]Alvarez-Jubete L, Wijngaard H, Arendt E K, Gallagher E.2010. Polyphenol composition and in vitro antioxidantactivity of amaranth, quinoa buckwheat and wheat asaffected by sprouting and baking. Food Chemistry, 119,770-778[3]Babbar N, Oberoi H S, Uppal D S, Patil R T. 2011. Totalphenolic content and antioxidant capacity of extractsobtained from six important fruit residues. FoodResearch International, 44, 391-396[4]Dykes L, Rooney L W. 2007. Phenolic compounds in cerealgrains and their health benefits. Cereal Foods World,3, 105-111[5]Deleu C Q, Gressier B, Vasseur J, Dine T, Brunet C, LuyckxM, Cazin M, Cazin J C, Bailleul F, Trotin F. 2000. Phenoliccompounds and antioxidant activities of buckwheat(Fagopyrum esculentum Moench) hulls and flour.Journal of Ethnopharmacology, 72, 35-42[6]Eva I, Ondrejovic M, Silhar S. 2012. Antioxidant activity ofmilling fractions of selected cereals. NovaBiotechnologica et Chimica, 11, 45-56[7]Ferreira I C F R, Baptista P, Vilas-Boas M, Barros L. 2007.Free-radical scavenging capacity and reducing powerof wild edible mushrooms from northeast Portugal:Individual cap and stipe activity. Food Chemistry, 100,1511-1516[8]Guo X D, Ma Y J, Parry J, Gao J M, Yu L L, Wang M. 2011.Phenolics content and antioxidant activity of tartarybuckwheat from different locations. Molecules, 16,9850-9867[9]Hodzic Z, Pasalic H, Memisevic A, Srabovic M, SaletovicM, Poljakovic M. 2009. The influence of total phenolscontent on antioxidant capacity in the whole grainextracts. European Journal of Scientific Research, 28,471-477[10]Holasovaa M, Fiedlerovaa V, Smrcinovaa H, Orsakb M,Lachmanb J, Vavreinova S. 2002. Buckwheat - thesource of antioxidant activity in functional foods. FoodResearch International, 35, 207-211[11]Hung P V, Morita N. 2008. Distribution of phenoliccompounds in the graded flours milled from wholebuckwheat grains and their antioxidant capacities. FoodChemistry, 109, 325-331[12]Inglett G E, Chen D, Berhowa M, Lee S. 2011. Antioxidantactivity of commercial buckwheat flours and their freeand bound phenolic compositions. Food Chemistry,125, 923-929[13]Kaur C, Kapoor H C. 2000. Antioxidant activity and totalphenolic content of some Asian vegetables.International Journal of Food Science andTechnology, 37, 153-161[14]Kong S, Lee J. 2010. Antioxidants in milling fractions ofblack rice cultivars. Food Chemistry, 120, 278-281[15]Morishita T, Yamaguchi H, Konosuke D. 2007. Thecontribution of polyphenols to antioxidative activity incommon buckwheat and tartary buckwheat grain. PlantProduction Science, 10, 99-104[16]Oomah B D, Campbell C G, Mazza G. 1996. Effects of cultivarand environment on phenolic acids in buckwheat.Euphytica, 90, 73-77[17]Pereez-Jimenez J, Torres J L. 2011. Analysis of nonextractablephenolic compounds in foods: the currentstate of the art. Journal of Agricultural and FoodChemistry, 59, 12713-12724[18]Peng Y Y, Liu F H, Ye J N. 2004. Determination of phenoliccompounds in the hull and flour of buckwheat(Fagopyrum esculentum Möench) by capillaryelectrophoresis with electrochemical detection.Analytical Letters, 37, 2789-2803[19]Rockenbach I I, Rodrigues E, Gonzaga L V, Caliari V,Genovese M I, Gonçalves A E S S, Fett R. 2011. Phenoliccompounds content and antioxidant activity in pomacefrom selected red grapes (Vitis vinifera L. and Vitislabrusca L.) widely produced in Brazil. Food Chemistry,127, 174-179[20]Sedej I, Sakaè M, Mandiæ A, Mišan A, Tumbas V,Canadanoviæ-Brunet J. 2012. Buckwheat (Fagopyrumesculentum Moench) grain and fractions: antioxidantcompounds and activities. Journal of Food Science,77, C954-C959.Sharma P, Ghimeray A K, Gurung A, Jin C W, Rho H S, ChoD H. 2012. Phenolic contents, antioxidant and ?-glucosidase inhibition properties of Nepalese strainbuckwheat vegetables. African Journal ofBiotechnology, 11, 184-190[21]Sedej I J, Sakac M B, Misan A C. Mandic A I. 2010.Antioxidant activity of wheat and buckwheat flours.Zbornik Matice Srpske za Prirodne Nauke, 2010, 59-68[22]Sun T, Ho C T. 2005. Antioxidant activities of buckwheatextracts. Food Chemistry, 90, 743-749[23]Vaher M, Matso K, Levandi T, Helmja K, Kaljurand M.2010. Phenolic compounds and the antioxidant activityof the bran, flour and whole grain of different wheatvarieties. Procedia Chemistry, 2, 76-82[24]Verardo V, Arráez-Román D, Segura-Carretero A, MarconiE, Fernández-Gutiérrez A, Caboni A F. 2010.Identification of buckwheat phenolic compounds byreverse phase high performance liquid chromatographyelectrosprayionization-time of flight-mass spectrometry(RP-HPLC-ESI-TOF-MS). Journal of Cereal Science,52, 170-176[25]Zieliñski H, Kozlowska H. 2000. Antioxidant activity andtotal phenolics in selected cereal grains and theirdifferent morphological fractions. Journal ofAgriculture Food Chemistry, 48, 2008-2016 |
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