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| Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze |
| HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin |
1、College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China |
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摘要 In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis (L.) Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root of F. bidentis (L.) Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and 1H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as α-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.
Abstract In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis (L.) Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root of F. bidentis (L.) Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and 1H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as α-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.
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Received: 22 January 2013
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31171877). |
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Corresponding Authors:
ZHANG Jin-lin, Tel: +86-312-7528575, E-mail: zhangjinlin@hebau.edu.cn
E-mail: zhangjinlin@hebau.edu.cn
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| About author: HUO Jing-qian, E-mail: huojingqian@163.com |
Cite this article:
HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin.
2014.
Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze. Journal of Integrative Agriculture, 13(4): 804-810.
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| Ananvoranich S, Varin L, Gulick P, Ibrahim R. 1994. Cloning and regulation of flavonol 3-sulfotransferase in cell-suspension cultures of Flaveria bidentis. Plant Physiology, 106, 485-491 Batish D R, Arora K, Singh H P, Kohli R K. 2007. Potential utilization of dried powder of Tagetes minuta as a natural herbicide for managing rice weeds. Crop Protection, 26, 566-571 Chai Q, Huang G B, Huang P, Zhang E H, Feng F X. 2005. Identification of cicer arietinum root exudation and allelopathy of benzaldhyde. Acta Prataculturae Sinica, 14, 106-111 (in Chinese) David K W, Larhy J Z, Cahl D W, James E B, Wolfgang B, James E T. 1997. Toxicity of fractionated and degraded Mexican Marigold floral extract to adult Sitophilus zeamais (Coleoptera: Curculionidae). Journal of Econo Micentomology, 90, 1678-1683 Dayan F E, Cantrell C L, Duke S O. 2009. Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17, 4022-4034 Duke S O, Cantrell C L, Meepagala K M, Wedge D E, Tabanca N, Schrader K K. 2010. Natural toxins for use in pest management. Toxins, 2, 1943-1962 Gao X M, Tang T G, Liang Y, Zheng T X, Sang W G, Chen Y L. 2004. An alert regarding biological invasion by a new exotic plant, Flaveria bidentis, and strategies for its control. Chinese Biodiversity, 12, 274-279 Gidda S K, Varin L. 2006. Biochemical and molecular characterization of flavonoid 7-sulfotransferase from Arabidopsis thaliana. Plant Physiology and Biochemistry, 44, 628-636 Guglielmone H A, Agnese A M, Núñez Montoya S C, Cabrera J L. 2005. Inhibitory effects of sulphated flavonoids isolated from Flaveria bidentis on platelet aggregation. Thrombosis Research, 115, 495-502 Liao M, Xie X M, Peng Y, Ma A L. 2011. Changes of soil microbiological characteristics after Solidago canadensis L. invasion. Agricultural Sciences in China, 10, 1064- 1071. Mares D, Tosi B, Poli F, Andreotti E, Romagnoli C. 2004. Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: ultrastructural evidence on Pythium ultimum. Microbiological Research, 159, 295- 304. Marotti I, Marotti M, Piccaglia R, Nastri A, Grandi S, Dinelli G. 2010. Thiophene occurrence in different Tagetes species: agricultural biomasses as sources of biocidal substances. Journal of the Science of Food and Agriculture, 90, 1210-1217 Paterson I, Anderson E A. 2005. The renaissance of natural products as drug candidates. Science, 310, 451-453 Ren Y P, Jiang S, Gu S, Wang Y Z, Zheng S X. 2008. Advances in Flaveria bidentis (L.) Kuntze, a new exotic plant. Journal of Tropical and Subtropical Botany, 16, 390-396 Stuebler H, Kraehmer H, Hess M. 2008. Global changes in crop production and impact trends in weed management - an industry view. In: Proceedings of the 5th International Weed Science Congress. University of Georgia Press, Vancouver, Canada. pp. 309-319 Wang X G, Wei X Y, Huang X Y, Shen L T, Tian Y Q, Xu H H. 2011. Insecticidal constructure and bioactivities of compounds from Ficus sarmentosa var. henryi. Agricultural Sciences in China, 10, 1402-1409 Xie Q Q, Wei Y, Zhang G L. 2010. Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high- speed counter-current chromatography. Separation and Purification Technology, 72, 229-233 Xu J, Xu W C, Yang Y J, Tao B, Zhang J L. 2008. The allelopathy of Flaveria bidentis (L.) Kuntze, an invasive weed species. Frontiers of Agriculture in China, 2, 446- 450. Zhang F J, Guo J Y, Chen F X, Guo A Y, Wan F H. 2012. Assessment of allelopathic effects of residues of Flaveria bidentis (L.) Kuntze on wheat seedlings. Archives of Agronomy and Soil Science, 58, 257-265 Zhang J L, Zhang L H, Liu Y C, Ma J, Li C, Dong J G. 2006. The herbicidal activity of mutant isolates from Botrytis cinerea. Agricultural Sciences in China, 5, 622- 628. |
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