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| Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis |
| Key Laboratory of Dairy Science, Ministry of Education/Life College, Northeast Agricultural University |
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摘要 In previous experiment, we isolated a compound dibutyl phthalate (DBP) from Vaccaria segetalis which had galactopoieticfunction on mammary gland epithelial cells of dairy cow (DCMECs). In this experiment, we ascertained the metabolicregulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, â-casein, Glut1, SREBP-1,PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot andimmunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 andp-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates â-casein synthesis. DBP also raises theactivities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPKdownstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, theactivities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolismlevel of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs.
Abstract In previous experiment, we isolated a compound dibutyl phthalate (DBP) from Vaccaria segetalis which had galactopoieticfunction on mammary gland epithelial cells of dairy cow (DCMECs). In this experiment, we ascertained the metabolicregulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, â-casein, Glut1, SREBP-1,PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot andimmunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 andp-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates â-casein synthesis. DBP also raises theactivities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPKdownstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, theactivities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolismlevel of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs.
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Accepted:
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Corresponding Authors:
Correspondence GAO Xue-jun, Professor, Tel: +86-451-55190244, Fax: +86-451-55190244, E-mail:gaoxj5390@sina.com
E-mail: tonghuili2004@163.com
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| About author: TONG Hui-li, Ph D, E-mail: tonghuili2004@163.com |
Cite this article:
TONG Hui-li, GAO Xue-jun, LI Qing-zhang, LIU Jie, LI Nan, WAN Zhong-ying.
2011.
Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis. Journal of Integrative Agriculture, 10(7): 1106-1116.
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