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| Immunoproteomic Assay of Antigenic Surface Proteins in Streptococcus equi ssp. zooepidemicus |
| 1Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture/College of Veterinary Medicine, Nanjing Agricultural University |
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摘要 Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is a zoonotic pathogen with worldwide distribution. Lackingsuitable vaccine and virulent maker is still bottleneck to control this infection. An immunoproteomic approach has beenused to screen the membrane-associated and cell wall-associated proteins of S. zooepidemicus isolate in China CY todiscover vaccine candidate antigens and therapeutic agents. Finally, 11 membrane-associated proteins, and 13 cell wallassociatedproteins were successfully identified. BLAST (www.sanger.ac.uk) results also indicated that nucleotidesequences of majority identified proteins shared high homology (>60%) with S. zooepidemicus, except for AC1-3, AC5,AC8, and AC13. Moreover, genes for 7 of the identified proteins were detected from CY; compared with ST171, 3 of them(AM1, AM8 and AC11) were only found in virulent strains (CY). All of the proteins identified in this study remain not tobe reported in S. zooepidemicus. Some of the proteins serve a vital role in the immune system and reproduction of hostspecies according to available data, while the functions of the rest were seldom researched.
Abstract Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is a zoonotic pathogen with worldwide distribution. Lackingsuitable vaccine and virulent maker is still bottleneck to control this infection. An immunoproteomic approach has beenused to screen the membrane-associated and cell wall-associated proteins of S. zooepidemicus isolate in China CY todiscover vaccine candidate antigens and therapeutic agents. Finally, 11 membrane-associated proteins, and 13 cell wallassociatedproteins were successfully identified. BLAST (www.sanger.ac.uk) results also indicated that nucleotidesequences of majority identified proteins shared high homology (>60%) with S. zooepidemicus, except for AC1-3, AC5,AC8, and AC13. Moreover, genes for 7 of the identified proteins were detected from CY; compared with ST171, 3 of them(AM1, AM8 and AC11) were only found in virulent strains (CY). All of the proteins identified in this study remain not tobe reported in S. zooepidemicus. Some of the proteins serve a vital role in the immune system and reproduction of hostspecies according to available data, while the functions of the rest were seldom researched.
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Received: 26 July 2010
Accepted:
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Corresponding Authors:
Correspondence FAN Hong-jie, Professor, Tel: +86-25-843965328, Fax: +86-25-84396517, E-mail: fhj@njau.edu.cn
E-mail: maoying_00@yahoo.com.cn
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| About author: MAO Ying, MSc, E-mail: maoying_00@yahoo.com.cn |
Cite this article:
MAO Ying, FAN Hong-jie, ZHOU Yong-hua, LU Cheng-ping.
2011.
Immunoproteomic Assay of Antigenic Surface Proteins in Streptococcus equi ssp. zooepidemicus. Journal of Integrative Agriculture, 10(7): 1096-1105.
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