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| The Effect of Glycyl-Glutamine Dipeptide Concentration on Enzyme Activity,Cell Proliferation and Apoptosis of Jejunal Tissues from Weaned Piglets |
| Institute of Animal Nutrition, Sichuan Agricultural University |
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摘要 An experiment was conducted in a singly factorial design to study the effect of glycyl-glutamine dipeptide on enzymeactivity, cell proliferation and apoptosis of jejunal tissues from weaned piglets at different glycyl-glutamine concentrationlevels of 2, 4, 10, 20, and 30 mmol L-1, respectively. The glutaminase activity, diamine oxidase (DAO) activity, cellpeoliferation, apoptosis, and perotein metabolism were measured by the tissue culture method in vitro using jejunaltissues. The immunohistochemical method was used to study the cell proliferation and apoptosis of jejunal tissues. Theresults showed that compared to the blank control, the percentage and MOD value of BrdU-positicve cells incubated withglycyl-glutamine dipeptide solution were significantly (P<0.05) increased. Accordingly, the percentage and MOD valueof caspase-3-positive cells from tissue incubated with glycyl-glutamine dipeptide were notably lower (P<0.05) than thatfrom the control treatment. The glycyl-glutamine dipeptide increased the glutaminase activity, DAO activity and proteincontent of jejunal tissues, as the dipeptide concentration was on the rise (P<0.05). These results indicated that glycylglutaminedipeptide affected the jejunum development and adaptation of weaned piglets, and the function might befulfilled by enhancing the glutamine-related enzyme activity, thereby increasing the consumption of glutamine, and thenimproving the jejunal cell proliferation and suppressing cell apoptosis. The effects of glycyl-glutamine dipeptide relied ina dose-dependent manner, and the maximum effect was achieved at 20-30 mmol L-1 glycyl-glutamine dipeptide.
Abstract An experiment was conducted in a singly factorial design to study the effect of glycyl-glutamine dipeptide on enzymeactivity, cell proliferation and apoptosis of jejunal tissues from weaned piglets at different glycyl-glutamine concentrationlevels of 2, 4, 10, 20, and 30 mmol L-1, respectively. The glutaminase activity, diamine oxidase (DAO) activity, cellpeoliferation, apoptosis, and perotein metabolism were measured by the tissue culture method in vitro using jejunaltissues. The immunohistochemical method was used to study the cell proliferation and apoptosis of jejunal tissues. Theresults showed that compared to the blank control, the percentage and MOD value of BrdU-positicve cells incubated withglycyl-glutamine dipeptide solution were significantly (P<0.05) increased. Accordingly, the percentage and MOD valueof caspase-3-positive cells from tissue incubated with glycyl-glutamine dipeptide were notably lower (P<0.05) than thatfrom the control treatment. The glycyl-glutamine dipeptide increased the glutaminase activity, DAO activity and proteincontent of jejunal tissues, as the dipeptide concentration was on the rise (P<0.05). These results indicated that glycylglutaminedipeptide affected the jejunum development and adaptation of weaned piglets, and the function might befulfilled by enhancing the glutamine-related enzyme activity, thereby increasing the consumption of glutamine, and thenimproving the jejunal cell proliferation and suppressing cell apoptosis. The effects of glycyl-glutamine dipeptide relied ina dose-dependent manner, and the maximum effect was achieved at 20-30 mmol L-1 glycyl-glutamine dipeptide.
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Received: 15 July 2010
Accepted:
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Corresponding Authors:
Correspondence JIA Gang, Professor, Ph D, Tel: +86-835-2885005, E-mail: jiagang700510@163.com
E-mail: E-mail: fungia@163.com
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| About author: WANG Hui, MSc, E-mail: fungia@163.com |
Cite this article:
WANG Hui, JIA Gang, CHEN Zheng-li, HUANG Lan, WU Cai-mei, WANG Kang-ning.
2011.
The Effect of Glycyl-Glutamine Dipeptide Concentration on Enzyme Activity,Cell Proliferation and Apoptosis of Jejunal Tissues from Weaned Piglets. Journal of Integrative Agriculture, 10(7): 1088-1095.
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| [1] Baylin S B, Stevens S A, Shakir K M. 1978. Association ofdiamine oxidase and ornithine decarboxylase with maturingcells in rapidly proliferating epithelium. Biochimica etBiophysica Acta, 541, 415-419.[2] Boudry G, Péron V, le Huërou-Luron I, Lallès J P, Sève B. 2004.Weaning induces both transient and long-lasting modificationsof absorptive, secretory, and barrier properties of pigletintestine. Journal of Nutrition, 134, 2256-2262.[3] Christie A, Butler M. 1994. Glutamine-based dipeptides areutilized in mammalian cell culture by extracellular hydrolysiscatalyzed by a specific peptidase. Journal of Biotechnology,37, 277-290.[4] Coeffer M, Dechelotte P. 2005. The role of glutamine in intensivecare unit patients: mechanisms of action and clinical outcome.Nutrition Reviews, 63, 65-69.[5] Dechelotte P, Hasselmann M, Cynober L, Allaouchiche B, CoefferM, Hecketsweiler B, Merle V, Mazerolles M, Samba D,Guillou Y M, et al. 2006. L-Alanyl-L-glutamine dipeptidesupplementedtotal parenteral nutrition reduces infectiouscomplications and glucose intolerance in critically ill patients:the French controlled randomised double-blind multicenterstudy. Critical Care Medicine, 34, 598-604.[6] Erbil Y, Oztezcan S, Giris M, Barbaros U, Olgac V, Bilge H,Kucucuk H, Toker G. 2005. The effect of glutamine onradiation-induced organ damage. Life Sciences, 78, 376-382.[7] Haque S M, Chen K, Usui N, Iiboshi Y, Okuyama H, MasunariA, Cui L, Nezu R, Takagi Y. 1996. Alanyl-glutaminedipeptide-supplemented parenteral nutrition improvesintestinal metabolism and prevents increased permeability in rats. Annals of Surgery, 223, 334-341.[8] Janicke R U, Sprengart M L, Wati M R, Porter A G. 1998.Caspase-3 is required for DNA fragmentation andmorphological changes associated with apoptosis. TheJournal of Biological Chemistry, 273, 9357-9360.[9] Jilling T, Lu J, Jackson M, Caplan M S. 2004. Intestinal epithelialapoptosis initiates gross bowel necrosis in an experimentalmodel of neonatal necrotizing enterocolitis. PediatricResearch, 55, 622-629.[10] Köhler H, Hartig-Knecht H, Rüggeberg J, Adam R, Schroten H.2000. Lymphocyte proliferation is possible with lowconcentrations of glycyl-glutamine. European Journal ofNutrition, 39, 103-105.[11] Legrand C, Bour J M, Jacob C, Capiaumont J, Martial A, MarcA, Wudtke M, Kretzmer G, Demangel C, Duval D. 1992.Lactate dehydrogenase (LDH) activity of the culturedeukaryotic cells as marker of the number of dead cells in themedium. Journal of Biotechnology, 25, 231-243.[12] Li Y S, Li J S, Jiang J W, Li N, Wang X B,Wang Z M, Wu B, LiuF N. 2002. Glycyl-glutamine-supplemented long-term totalparenteral nutrition selectively improves structure andfunction in heterotopic small-bowel autotransplantation inthe pig. Transplant International, 16, 866-871.[13] Melis G C, ter Wengel N, Boelens P G, van Leeuwen P A. 2004.Glutamine: recent developments in research on the clinicalsignificance of glutamine. Current Opinion in ClinicalNutrition & Metabolic Care, 7, 59-70.[14] Scheppach W, Loges C, Bartram P, Christl S U, Richter F, DuselG, Stehle P, Fuerst P, Kasper H. 1994. Effect of freeglutamine and alanyl-glutamine dipeptide on mucosalproliferation of the human ileum and colon. Gastroenterology,107, 429-34.[15] Stehle P, Fuerst P. 2002. Effect of glutamine and glutaminedipeptide on mucosal of the human ileum and colon in vitro.Chinese Journal of Clinical Nutrition, 1, 1-5.(inChinese)[16] Tamada H, Nezu R, Matsuo Y, Imamura I, Takagi Y, Okada A.1992. Alanyl-glutamine enriched total paernteral nutritionrestores intestinal adaption after either proximal or distalmassive resection in rats. Journal of Parenteral and EnteralNutrition, 16, 110-116.[17] Tazuke Y, Wasa M, Shimizu Y, Wang H S, Okada A. 2003.Alanyl-glutamine-supplemented parenteral nutritionprevents intestinal ischemia-reperfusion injury in rats.Journal of Parenteral and Enteral Nutrition, 27, 110-115.[18] Wu G, Knabe D A. 1994. Free and protein-bound amino acids insow’s colostrum and milk. Journal of Nutrition, 124, 415-424. |
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