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| Phagocytic Uptake of Nosema bombycis (Microsporidia) Spores by Insect Cell Lines |
| CAI Shun-feng, LU Xing-meng, QIU Hai-hong, LI Ming-qian, FENG Zhen-zhen |
| Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310058, P.R.China |
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摘要 Microsporidia are highly specialized obligate intracellular parasites that can infect a wide variety of animals ranging from protists to mammals. The classical concept of the parasite invasion into a host cell involves its polar tube acting as a needle-syringe system. However, recent studies show microsporidian spores can also gain access to host cells by phagocytosis. The present study investigated the phagocytic uptake process of causative agent of the pebrine disease, Nosema bombycis, in several insect cell lines. We observed KOH-treated spores and cold-storaged spores can be easily uptaken by all the studied cell types 4 h post inoculation. In contrast, large numbers of freshly recovered spores remained in the culture medium. To further investigate the intracellular fates of KOH-treated spores and cold-storaged spores, electron and fluorescence microscopy were performed. No intracellular germination or subsequent parasite development were observed. Intracellular spores can be detected in host cells by polyclonal antibody 7 d post inoculation, suggesting phagocytized N. bombycis could not be digested by these non-professional phagocytes. Our results suggest that, phagocytic uptake of N. bombycis spores might represent a defense mechanism of the host cells and the intact spore wall barrier enable freshly recovered spores to keep resistance to this mechanism.
Abstract Microsporidia are highly specialized obligate intracellular parasites that can infect a wide variety of animals ranging from protists to mammals. The classical concept of the parasite invasion into a host cell involves its polar tube acting as a needle-syringe system. However, recent studies show microsporidian spores can also gain access to host cells by phagocytosis. The present study investigated the phagocytic uptake process of causative agent of the pebrine disease, Nosema bombycis, in several insect cell lines. We observed KOH-treated spores and cold-storaged spores can be easily uptaken by all the studied cell types 4 h post inoculation. In contrast, large numbers of freshly recovered spores remained in the culture medium. To further investigate the intracellular fates of KOH-treated spores and cold-storaged spores, electron and fluorescence microscopy were performed. No intracellular germination or subsequent parasite development were observed. Intracellular spores can be detected in host cells by polyclonal antibody 7 d post inoculation, suggesting phagocytized N. bombycis could not be digested by these non-professional phagocytes. Our results suggest that, phagocytic uptake of N. bombycis spores might represent a defense mechanism of the host cells and the intact spore wall barrier enable freshly recovered spores to keep resistance to this mechanism.
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Received: 28 April 2011
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (30771456). |
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Corresponding Authors:
Correspondence LU Xing-meng, Mobile: 13605718106, Fax: +86-571-86971697, E-mail:xmlu@zju.edu.cn
E-mail: xmlu@zju.edu.cn
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| About author: CAI Shun-feng, Mobile: 13757127487, E-mail: caishunfeng@gmail.com; |
Cite this article:
CAI Shun-feng, LU Xing-meng, QIU Hai-hong, LI Ming-qian, FENG Zhen-zhen.
2012.
Phagocytic Uptake of Nosema bombycis (Microsporidia) Spores by Insect Cell Lines. Journal of Integrative Agriculture, 12(8): 1321-1326.
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