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1. 转录组和脂质组联合分析揭示棉花纤维中低K+信号缓解缺Ca2+效应的机制
GUO Kai, GAO Wei, ZHANG Tao-rui, WANG Zu-ying, SUN Xiao-ting, YANG Peng, LONG Lu, LIU Xue-ying, WANG Wen-wen, TENG Zhong-hua, LIU Da-jun, LIU De-xin, TU Li-li, ZHANG Zheng-sheng
Journal of Integrative Agriculture    2023, 22 (8): 2306-2322.   DOI: 10.1016/j.jia.2023.01.002
摘要194)      PDF    收藏

Ca2+离子在维持细胞壁以及细胞膜的完整性中具有重要作用,是植物生长和发育中必不可少的矿质营养元素之一。解析Ca2+离子在糖代谢和脂代谢中的作用能够为理解棉花纤维快速伸长阶段细胞膜和细胞壁的动态变化提供有意义的参考。本研究利用胚珠培养系统发现缺Ca2+会促进纤维和胚珠细胞的膨大,但同时也会诱导组织的褐化。RNA-seq差异表达基因分析发现缺Ca2+使细胞处于一个较高的氧化态,并且激活与糖代谢和脂代谢相关的基因的表达。尤其以糖酵解途径变化最为显著,其代谢途径中的9个酶相关的基因上调表达,缺Ca2+处理细胞中的葡萄糖含量显著下降,改变了糖酵解途径的流动。低K+能够恢复缺Ca2+诱导糖酵解途径相关基因的表达以及葡萄糖的含量。采用电喷雾电离串联质谱技术检测了不同Ca2+K+离子处理条件下细胞中脂质组成分的动态变化。缺Ca2+处理细胞中自由脂肪酸(FA)、二酰甘油(DAG)和糖脂含量降低,三酰甘油(DAG)磷脂酰乙醇胺(PE)、磷脂酰甘油(PG)、磷脂酰胆碱(PC含量增加。低K+与缺Ca2+的互作信号能够恢复FA、磷脂、糖脂含量至正常水平,有效缓解缺Ca2+效应。本研究通过在转录和代谢水平的比较分析,揭示了Ca2+K+信号互作在维持纤维快速伸长过程中糖酵解和脂代谢中发挥着重要作用。

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2. 甘薯小象甲侵害诱导的(Z)-3-己烯基乙酯和别罗勒烯的昼夜节律性释放激活甘薯对甘薯小象甲的防御反应
XIAO Yang-yang, QIAN Jia-jia, HOU Xing-liang, ZENG Lan-ting, LIU Xu, MEI Guo-guo, LIAO Yin-yin
Journal of Integrative Agriculture    2023, 22 (6): 1782-1796.   DOI: 10.1016/j.jia.2023.02.020
摘要206)      PDF    收藏

甘薯小象虫(Cylas formicarius Fab.)(Coleoptera: Brentidae))是一种以甘薯(Ipomoea batatasL.Lam.Solanales: Convolvulaceae))为食的害虫,每年造成巨大的经济损失。然而,目前还没有找到安全有效的方法来保护甘薯免受甘薯小象甲的侵害。昆虫侵害诱导的植物挥发物(HIPVs)能激活多种防御的生物活性,但它们在甘薯中的形成和防御机制仍未清晰。为了明确甘薯中合成的防御性HIPVs,我们监测了虫侵害过程中甘薯挥发物的释放动态。通过稳定同位素示踪以及转录和代谢水平的分析,揭示了候选HIPVs的生物合成途径和调控因子。最后,对候选HIPVs的抗虫活性和防御机制进行了评估。本研究表明,(Z)-3-己烯基乙酯(z3HAC)和别罗勒烯由甘薯小象甲诱导合成,具有明显的昼夜节律。本文还首次报道了Ipomoea batatas ocimene synthaseIbOS)是别罗勒烯合成路径的基因。昆虫侵害造成的损伤促进了底物 (Z)-3-己烯醇的积累,并上调了IbOS的表达,从而分别导致z3HAC和别罗勒烯含量的增加。z3HAC和别罗勒烯气体分子能激活临近植株对甘薯小象甲的防御能力。本研究提供了关于甘薯防御性挥发物的形成、调控和信号转导机制的信息,对于建立有效的甘薯小象甲防治措施具有重要意义。

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3. 玉米冠根性状的遗传解析及其与地上部农艺性状的遗传关系
SHA Xiao-qian, GUAN Hong-hui, ZHOU Yu-qian, SU Er-hu, GUO Jian, LI Yong-xiang, ZHANG Deng-feng, LIU Xu-yang, HE Guan-hua, LI Yu, WANG Tian-yu, ZOU Hua-wen, LI Chun-hui
Journal of Integrative Agriculture    2023, 22 (11): 3394-3407.   DOI: 10.1016/j.jia.2023.04.022
摘要238)      PDF    收藏

冠根系统是玉米营养期和生殖期最重要的根系组成部分。然而,玉米冠根性状的遗传基础及其与地上部农艺性状的关系尚不清楚。本研究以531个玉米优良自交系为研究对象,在不同的田间环境下,对其冠根相关性状和地上部农艺性状进行表型分析。结果表明,根系性状与开花时间、株型结构、籽粒产量等地上部农艺性状呈显著正相关。通过全基因组关联分析(GWAS)结合重测序,共鉴定出115关联位点和22个高置信候选基因。其中冠根与花期和植株构型有46QTL共定位,因此大约三分之一的冠根性状遗传变异可能要归因于开花时间和植株结构。此外,115个冠根位点中有103个89.6%)位于已知的驯化和改良选择范围内,这表明冠根在玉米驯化和改良过程中可能经历了间接选择。此外,Zm00001d036901是一个高置信候选基因,其表达可能与玉米冠根的表型变异有关,Zm00001d036901在玉米驯化改良过程中是受选择的。本研究促进了我们对根系结构遗传基础的理解,并为改进玉米根系结构提供了基因组学资源。

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4. The comparative analysis and identification of secondary metabolites between Tibet wild and cultivated pomegranates (Punica granatum L.) in China
GUO Lin-hui, GE Da-peng, REN Yuan, DONG Jian-mei, ZHAO Xue-qing, LIU Xue-qing, YUAN Zhao-he
Journal of Integrative Agriculture    2022, 21 (3): 736-750.   DOI: 10.1016/S2095-3119(21)63642-0
摘要151)      PDF    收藏

次生代谢物质与植物的营养品质和保健功能密切相关。本研究首次收集了国内23个野生石榴和27个栽培石榴的成熟果实,比较分析了不同种质石榴果皮和果汁中黄酮和鞣质的含量差异,并采用液相色谱-电喷雾串联质谱法(LC-ESI-MS/MS)法测定了果汁高黄酮种质‘则拉4’果皮(ZLP)和果汁(ZLZ)中的次生代谢物质。方差分析(P<0.05)表明,不同石榴种质间的黄酮和鞣质含量存在显著差异。Pearson相关分析表明,影响石榴黄酮和鞣质含量的主要环境因子是纬度和海拔。本研究在‘则拉4’果皮和果汁中共鉴定出279种次生代谢物质,其中227种次生代谢物首次在石榴中发现。通过正交偏最小二乘判别分析法,在ZLP和ZLZ中筛选出了90种差异代谢物。本研究还筛选了8种特异性种质资源(果皮高黄酮,‘军拥3’;果皮低黄酮,‘胭脂红’;果汁高黄酮,‘则拉4’;果汁低黄酮,‘豫大籽’,果皮高鞣质,‘军拥4’,果皮低鞣质,‘安巴1’,果汁高鞣质,‘叶巴1’,果汁低鞣质,‘白花玉石籽’。本研究结果可为我国野生石榴资源的开发利用和石榴育种提供参考。


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5. JIA-2021-0833 灌浆结实期水分调节通过对弱势粒淀粉合成的改善缓解高温胁迫对粳稻籽粒品质的危害
TONG Hui, DUAN Hua, WANG Sheng-jun, SU Jing-ping, SUN Yue, LIU Yan-qing, TANG Liang, LIU Xue-jun, CHEN Wen-fu
Journal of Integrative Agriculture    2022, 21 (10): 3094-3101.   DOI: 10.1016/j.jia.2022.07.012
摘要208)      PDF    收藏
温度和水分是影响水稻产量和品质形成的重要因子,灌浆结实期适度的干旱能够有效的缓解高温胁迫对水稻造成的伤害,但有关其淀粉合成的调控机理尚不清楚。为探明灌浆结实期高温与干旱对水稻淀粉合成的影响及其生理机制,本试验研究了4个不同处理下(对照(CK)、高温(HT)、适度干旱(WS)、高温干旱(HT+WS))常规粳稻淀粉相关酶活性及其基因的表达水平。结果表明高温和干旱对籽粒灌浆的影响主要发生在弱势粒中,并通过调节参与淀粉合成相关酶活性及其主效基因的表达水平,进而影响稻米淀粉的合成。高温和干旱有拮抗作用,适度干旱能够改善高温对稻米品质的影响。
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6. JIA-2021-0532 利用STTM技术抑制miR397提高甘薯对SPVD的抗性
LI Chen, LIU Xuan-xuan, ABOUELNASR Hesham, MOHAMED HAMED Arisha, KOU Meng, TANG Wei, YAN Hui, WANG Xin, WANG Xiao-xiao, ZHANG Yun-gang, LIU Ya-ju, GAO Run-fei, MA Meng, LI Qiang
Journal of Integrative Agriculture    2022, 21 (10): 2865-2875.   DOI: 10.1016/j.jia.2022.07.054
摘要203)      PDF    收藏
甘薯(Ipomoea batatas (L.) Lam.)作为一种重要的杂粮作物,在世界各地广泛种植,但深受甘薯病毒病(SPVD)的影响。本研究利用短串联靶标模拟物(STTM)成功抑制甘薯miR397的表达,上调其靶基因漆酶(IbLACs)的表达,使木质素合成途径的上游基因,包括苯丙氨酸解氨酶(PAL)、4-香豆酸辅酶A连接酶(4CL)、羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶(HTC)、咖啡酸O-甲基转移酶(COMT)、肉桂醇脱氢酶(CAD)等基因被反馈调控而广泛上调表达。同时,导致PAL和LAC的酶活性显著增加,促进木质素的合成与积累。木质素在细胞壁中的沉积增加了转基因甘薯植株的物理防御能力,有效减少了烟粉虱对SPVD的传播,保证甘薯的健康生长。本研究为甘薯抗病育种和绿色生产提供了新思路。
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7. Physiological response of flag leaf and yield formation of winter wheat under different spring restrictive irrigation regimes in the Haihe Plain, China
LIU Xue-jing, YIN Bao-zhong, HU Zhao-hui, BAO Xiao-yuan, WANG Yan-dong, ZHEN Wen-chao
Journal of Integrative Agriculture    2021, 20 (9): 2343-2359.   DOI: 10.1016/S2095-3119(20)63352-4
摘要158)      PDF    收藏

为了阐明海河平原冬小麦春季限制灌溉的最佳时期及其对旗叶衰老和产量形成的影响,2016年至2019年,研究组在河北农业大学辛集试验站进行了不同灌溉模式的田间试验,试验包括两种灌溉模式:对照(CK,分别在3叶展开期和开花期浇水)和单一限制灌溉(SRI),后者又包括3叶展开期(3LI)、4叶展开期(4LI)、5叶展开期(5LI)和6叶展开期(6LI)灌溉。结果表明:(1)与对照相比,4LI处理组在一定程度上衰老进程(用绿叶面积表示)提前,而5LI和6LI处理组之间的差异不显著,衰老发生明显晚于3LI处理组;(2)与其他SRI处理组相比,4LI处理组的GLA值和光合速率分别提高了14.82%和20.1%。旗叶显微结构分析还表明,干旱胁迫下3LI和6LI处理组的叶肉细胞和叶绿体排列不规则,但这种胁迫对4LI和5LI处理组的微观结构的负面影响很小;(3)春季延迟灌溉在籽粒灌浆前期可显著增加超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,随后酶的活性逐渐降低。在4个SRI处理组中,4LI处理组的总酶活性最高,4LI和5LI处理组的旗叶MDA含量平均比3LI和6LI处理组的MDA含量低14.5%;(4) 可溶性糖(SS)和脯氨酸(Pro)含量以4LI处理组最高,但低于CK组。4LI和5LI处理组的ABA激素含量低于3LI和6LI处理组,说明4LI和5LI处理组遭受的干旱胁迫程度较小;(5)在两个生长季,4LI处理组的单位面积穗数较多(比5LI和6LI处理组高13.4%),且千粒重最高(比其他三种SRI处理组高6.0%)。因此,4LI处理组的冬小麦产量在四个SRI处理组中最高。综合分析,建议4LI处理组(即在春季4叶期进行一次灌水)能有效延缓旗叶衰老进程,并使冬小麦保持相对较高的产量。


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8.
Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing
HAO Lu-yang, LIU Xu-yang, ZHANG Xiao-jing, SUN Bao-cheng, LIU Cheng, ZHANG Deng-feng, TANG Huai-jun, LI Chun-hui, LI Yong-xiang, SHI Yun-su, XIE Xiao-qing, SONG Yan-chun, WANG Tian-yu, LI Yu
Journal of Integrative Agriculture    2020, 19 (2): 449-464.   DOI: 10.1016/S2095-3119(19)62660-2
摘要174)      PDF    收藏
Drought is one of the most important abiotic stresses affecting maize growth and development and therefore resulting in yield loss.  Thus it is essential to understand molecular mechanisms of drought stress responses in maize for drought tolerance improvement.  The root plays a critical role in plants sensing water deficit.  In the present study, two maize inbred lines, H082183, a drought-tolerant line, and Lv28, a drought-sensitive line, were grown in the field and treated with different water conditions (moderate drought, severe drought, and well-watered conditions) during vegetative stage.  The transcriptomes of their roots were investigated by RNA sequencing.  There were 1 428 and 512 drought-responsive genes (DRGs) in Lv28, 688 and 3 363 DRGs in H082183 under moderate drought and severe drought, respectively.  A total of 31 Gene Ontology (GO) terms were significantly over-represented in the two lines, 13 of which were enriched only in the DRGs of H082183.  Based on results of Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis, “plant hormone signal transduction” and “starch and sucrose metabolism” were enriched in both of the two lines, while “phenylpropanoid biosynthesis” was only enriched in H082183.  Further analysis revealed the different expression patterns of genes related to abscisic acid (ABA) signal pathway, trehalose biosynthesis, reactive oxygen scavenging, and transcription factors might contribute to drought tolerance in maize.  Our results contribute to illustrating drought-responsive molecular mechanisms and providing gene resources for maize drought improvement.
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9. Transcriptomic profiling of sorghum leaves and roots responsive to drought stress at the seedling stage
ZHANG Deng-feng, ZENG Ting-ru, LIU Xu-yang, GAO Chen-xi, LI Yong-xiang, LI Chun-hui, SONG Yan-chun, SHI Yun-su, WANG Tian-yu, LI Yu
Journal of Integrative Agriculture    2019, 18 (9): 1980-1995.   DOI: 10.1016/S2095-3119(18)62119-7
摘要145)      PDF    收藏
Drought stress affects the growth and productivity of crop plants including sorghum.  To study the molecular basis of drought tolerance in sorghum, we conducted the transcriptomic profiling of sorghum leaves and roots under drought stress using RNA-Seq method.  A total of 510, 559, and 3 687 differentially expressed genes (DEGs) in leaves, 3 368, 5 093, and 4 635 DEGs in roots responding to mild drought, severe drought, and re-watering treatments were identified, respectively.  Among them, 190 common DEGs in leaves and 1 644 common DEGs in roots were responsive to mild drought, severe drought, and re-watering environment.  Gene Ontology (GO) enrichment analysis revealed that the GO categories related to drought tolerance include terms related to response to stimulus especially response to water deprivation, abscisic acid stimulus, and reactive oxygen species.  The major transcription factor genes responsive to drought stress include heat stress transcription factor (HSF), ethylene-responsive transcription factor (ERF), Petunia NAM, Arabidopsis ATAF1/2 and CUC2 (NAC), WRKY transcription factor (WRKY), homeodomain leucine zipper transcription factor (HD-ZIP), basic helix-loop-helix transcription factor (bHLH),  and V-myb myeloblastosis viral oncogene homolog transcription facotr (MYB).  Functional protein genes for heat shock protein (HSPs), late-embryogenesis-abundant protein (LEAs), chaperones, aquaporins, and expansins might play important roles in sorghum drought tolerance.  Moreover, the genomic regions enriched with HSP, expansin, and aquaporin genes responsive to drought stress could be used as powerful targets for improvement of drought tolerance in sorghum and other cereals.  Overall, our results provide a genome-wide analysis of DEGs in sorghum leaves and roots under mild drought, severe drought, and re-watering environments.  This study contributes to a better understanding of the molecular basis of drought tolerance of sorghum and can be useful for crop improvement.
 
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10. First detection and complete genome of Soybean chlorotic mottle virus naturally infecting soybean in China by deep sequencing
HU Qian-qian, LIU Xue-jian, HAN Xue-dong, LIU Yong, JIANG Jun-xi, XIE Yan
Journal of Integrative Agriculture    2019, 18 (11): 2664-2667.   DOI: 10.1016/S2095-3119(19)62665-1
摘要102)      PDF    收藏
Soybean chlorotic mottle virus (SbCMV) was first detected from soybean plants in Jiangxi Province of China by high throughput sequencing and was confirmed by PCR.  The complete nucleotide sequence of NC113 was determined to be 8 210 nucleotides, and shared the highest similarity (91.7%) with sequences of SbCMV that was only reported in Japan.  It encodes nine putative open reading frames (ORFs Ia, Ib and II–VIII), and contains a large intergenic region located at nucleotide 5 976–6 512 between ORFs VI and VII.  Sequence analysis and phylogenetic tree indicated that NC113 is an isolate of SbCMV, and is more related to the soymoviruses Blueberry red ringspot virus (BRRSV), Peanut chlorotic streak virus (PCSV) and Cestrum yellow leaf curling virus (CmYLCV) than to other representative members in the Caulimoviridae family.  Field survey of 472 legume plants from Jiangxi and Zhejiang provinces showed SbCMV was only detected from soybean in Nanchang City with a low incidence rate.  This is the first report of Soybean chlorotic mottle virus identified in China.
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11. Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks
LIU Xuan, LIANG Wei, LI Yu-xing, LI Ming-jun, MA Bai-quan, LIU Chang-hai, MA Feng-wang, LI Cui-ying
Journal of Integrative Agriculture    2019, 18 (10): 2264-2271.   DOI: 10.1016/S2095-3119(19)62706-1
摘要144)      PDF    收藏
Soil alkalinity is a major factor that restricts the growth of apple roots. To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.
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12. Development of a sensitive and reliable droplet digital PCR assay for the detection of ‘Candidatus Liberibacter asiaticus’
ZHONG Xi, LIU Xue-lu, LOU Bing-hai, ZHOU Chang-yong, WANG Xue-feng
Journal of Integrative Agriculture    2018, 17 (2): 483-487.   DOI: 10.1016/S2095-3119(17)61815-X
摘要733)      PDF    收藏
Citrus Huanglongbing (HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide.  To better improve the detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus’ (Las), the putative causal agent of HLB.  The detection of sensitivity comparison using positive plasmid indicated that ddPCR was superior to quantitative PCR (qPCR) for detecting and quantifying Las at low concentrations.  The Las detection of 40 field samples also showed that six of 13 asymptomatic samples (46.15%) with high Ct value (>35) were positive by ddPCR.  This methodology showed great potential for early HLB infection diagnosis.
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13. Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization
LIU Xue, ZHU Chang-xiong, Frank Delvigne, GONG Ming-bo
Journal of Integrative Agriculture    2017, 16 (09): 2110-2114.   DOI: 10.1016/S2095-3119(16)61477-6
摘要735)      PDF    收藏
    A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5´ end of the RNA transcript (SMART) technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length cDNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame (ORF) of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family (BI-1-like) proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L−1, which was associated with the release of 0.212 mg mL−1 of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate (P).
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14. Mapping of the heading date gene HdAey2280 in Aegilops tauschii
LIU Guo-xiang, ZHANG Li-chao, XIA Chuan, JIA Ji-zeng, ZHANG Jun-cheng, ZHANG Qiang, DONG Chun-hao, KONG Xiu-ying, LIU Xu
Journal of Integrative Agriculture    2016, 15 (12): 2719-2725.   DOI: 10.1016/S2095-3119(15)61291-6
摘要1016)      PDF    收藏
    An optimum heading date is essential for sustainable crop productivity and ensuring high yields. In the present study, F2:3 populations were generated by crossing an early-heading accession, Y2280, with a late-heading accession, Y2282. The heading dates of the F2 and F3 populations were investigated in a field study. Using publicly available simple sequence repeat (SSR) markers, the early heading date gene HdAey2280 was mapped onto Aegilops tauschii chromosome 7DS between the flanking markers wmc438 and barc126 at distances of 15 and 9.1 cM, respectively. Further analysis indicated that HdAey2280 is a novel heading date gene. New SSR markers were developed based on the Ae. tauschii draft genome sequence, resulting in four new markers that were linked to the heading date gene HdAey2280. The closest distance of these markers was 1.9 cM away from the gene. The results collected in this study will serve as a framework for map-based cloning and marker-assisted selection in wheat breeding programs in the future.
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15. New Food Safety Law of China and the special issue on food safety in China
Shu Geng, LIU Xu, Roger Beachy
Journal of Integrative Agriculture    2015, 14 (11): 2136-2141.   DOI: 10.1016/S2095-3119(15)61164-9
摘要1985)      PDF    收藏
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16. QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)
LI Guang-xian, CHEN Ai-hua, LIU Xu, WANG Wen-ying, DING Han-feng, LI Jun, LIU Wei, LI Si-shen , YAO Fang-yin
Journal of Integrative Agriculture    2014, 13 (11): 2311-2321.   DOI: 10.1016/S2095-3119(13)60615-2
摘要1524)      PDF    收藏
Heading date of rice is a key agronomic trait determining cultivated areas and seasons and affecting yield. In the present study, five primary single segment substitution lines with the same genetic background were used to detect quantitative trait loci (QTLs) for heading date in rice. Two QTLs, qHD3 and qHD6 on the short arm of chromosome 3 and the short arm of chromosome 6, respectively, were identified under natural long-day (NLD). Nineteen secondary single segment substitution lines (SSSLs) and seven double segments pyramiding lines were designed to map the two QTLs and to evaluate their epistatic interaction between them. By overlapping mapping, qHD3 was mapped in a 791-kb interval between SSR markers RM3894 and RM569 and qHD6 in a 1 125-kb interval between RM587 and RM225. Results revealed the existence of epistatic interaction between qHD3 and qHD6 under natural long-day (NLD). It was also found that qHD3 and qHD6 had significant effects on plant height and yield traits, indicating that both of the QTLs have pleiotropic effects.
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17. Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers
SONG Li-ya, LIU Xue, CHEN Wei-guo, HAO Zhuan-fang, BAI Li , ZHANG De-gui
Journal of Integrative Agriculture    2013, 12 (7): 1130-1137.   DOI: 10.1016/S2095-3119(13)60341-X
摘要1517)      PDF    收藏
Bulk-SSR method was used to analyze the genetic diversity of 44 open-pollinated varieties collected from Henan, Shandong, Shanxi, and Jilin provinces and Guangxi Zhuang Autonomous Region, China using 70 pairs of SSR primers. The purposes of this study were to (1) compare the genetic diversity among 44 Chinese maize open-pollinated varieties; (2) estimate the minimum number of alleles for construction of a stable dendrogram; and (3) trace the genetic relationships among local germplasm from different regions of China. In total, these 70 SSR primers yielded 292 alleles in 176 samples (4×44) analyzed. The number of alleles per locus was 4.17 on average and ranged from 2 to 8. The highest number of alleles per open-pollinated variety (55.25) was detected in Shanxi germplasm, which indicated that open-pollinated varieties from Shanxi possessed the largest genetic diversity among those from the five locations. The correlation coefficients between different genetic similarity matrices suggested that 200 alleles were sufficient for analysis of the genetic diversity of these 44 open-pollinated varieties. The cluster analysis showed that 44 open-pollinated varieties collected from three growing regions in China were accurately classified into three groups that were highly consistent with their geographic origins, and there is no correlation between GS and geographic distance in this study.
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18. PREFACE
LIU Xu
Journal of Integrative Agriculture    2012, 12 (1): 0-0.   DOI: 10.1016/S1671-2927(00)8530
摘要1218)      PDF    收藏
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19. Tribenuron-Methyl Resistant Shepherd’s Purse (Capsella bursa-pastoris (L.) Medik.) in Hebei Province of China
WANG Gui-qi, CUI Hai-lan, ZHANG Hong-jun, ZHANG Yan-qiu, LIU Xue, LI Xiang-ju, FAN Cuiqin
Journal of Integrative Agriculture    2011, 10 (8): 1241-1245.   DOI: 10.1016/S1671-2927(00)8396
摘要1807)      PDF    收藏
Shepherd’s purse seeds were collected from winter wheat fields and road sides in Hebei Province, China. Their sensitivitiesto tribenuron-methyl were evaluated in a whole plant dose response assay in the greenhouse. Results revealed that HB-1, HB-2, HB-3, HB-4, HB-6, HB-7, HB-17, HB-18, HB-19, HB-20, HB-21, and HB-22 populations were susceptible to tribenuronmethylcharacterised by very low GR50 values of about 0.10 g a.i. ha-1. On the other hand, populations HB-15 and HB-16had moderate resistance to tribenuron-methyl with resistance indices (RI) ranging from 10-100 folds, whilst populationsHB-5, HB-8, HB-9, HB-10, HB-11, HB-12, HB-13, and HB-14 expressed high level of resistance to tribenuron-methyl withresistance indices over 100. The proportion of moderate resistance level was about 9.09%, and that of high resistance wasabout 36.36%.
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