QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

doi:10.1016/S2095-3119(13)60615-2

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2311-2321.DOI: 10.1016/S2095-3119(13)60615-2

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QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

LI Guang-xian, CHEN Ai-hua, LIU Xu, WANG Wen-ying, DING Han-feng, LI Jun, LIU Wei, LI Si-shen , YAO Fang-yin

1、State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, P.R.China
3、Shandong Rice Research Institute, Ji’nan 250100, P.R.China

收稿日期:2013-07-06 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: LI Si-shen, Tel: +86-538-8242903, Fax: +86-538-8242226, E-mail: ssli@sdau.edu.cn;YAO Fang-yin, Tel: +86-531-83179440, Fax: +86-531-83178156, E-mail: yaofy@163.com
作者简介:LI Guang-xian, Mobile: 18766193972, E-mail: lgxrice@126.com
基金资助:
This work was financially supported by the National Natural Science Foundation of China (31171529), the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-13) and the Special Fund for Agro-Scientific Research in the Public Interest of China (201303007).

QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

LI Guang-xian, CHEN Ai-hua, LIU Xu, WANG Wen-ying, DING Han-feng, LI Jun, LIU Wei, LI Si-shen , YAO Fang-yin

1、State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, P.R.China
3、Shandong Rice Research Institute, Ji’nan 250100, P.R.China

Received:2013-07-06 Online:2014-11-06 Published:2014-11-14
Contact: LI Si-shen, Tel: +86-538-8242903, Fax: +86-538-8242226, E-mail: ssli@sdau.edu.cn;YAO Fang-yin, Tel: +86-531-83179440, Fax: +86-531-83178156, E-mail: yaofy@163.com
About author:LI Guang-xian, Mobile: 18766193972, E-mail: lgxrice@126.com
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31171529), the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-13) and the Special Fund for Agro-Scientific Research in the Public Interest of China (201303007).

摘要/Abstract

摘要： Heading date of rice is a key agronomic trait determining cultivated areas and seasons and affecting yield. In the present study, five primary single segment substitution lines with the same genetic background were used to detect quantitative trait loci (QTLs) for heading date in rice. Two QTLs, qHD3 and qHD6 on the short arm of chromosome 3 and the short arm of chromosome 6, respectively, were identified under natural long-day (NLD). Nineteen secondary single segment substitution lines (SSSLs) and seven double segments pyramiding lines were designed to map the two QTLs and to evaluate their epistatic interaction between them. By overlapping mapping, qHD3 was mapped in a 791-kb interval between SSR markers RM3894 and RM569 and qHD6 in a 1 125-kb interval between RM587 and RM225. Results revealed the existence of epistatic interaction between qHD3 and qHD6 under natural long-day (NLD). It was also found that qHD3 and qHD6 had significant effects on plant height and yield traits, indicating that both of the QTLs have pleiotropic effects.

关键词: rice (Oryza sativa L.) , single segment substitution lines , heading date , epistasis

Abstract: Heading date of rice is a key agronomic trait determining cultivated areas and seasons and affecting yield. In the present study, five primary single segment substitution lines with the same genetic background were used to detect quantitative trait loci (QTLs) for heading date in rice. Two QTLs, qHD3 and qHD6 on the short arm of chromosome 3 and the short arm of chromosome 6, respectively, were identified under natural long-day (NLD). Nineteen secondary single segment substitution lines (SSSLs) and seven double segments pyramiding lines were designed to map the two QTLs and to evaluate their epistatic interaction between them. By overlapping mapping, qHD3 was mapped in a 791-kb interval between SSR markers RM3894 and RM569 and qHD6 in a 1 125-kb interval between RM587 and RM225. Results revealed the existence of epistatic interaction between qHD3 and qHD6 under natural long-day (NLD). It was also found that qHD3 and qHD6 had significant effects on plant height and yield traits, indicating that both of the QTLs have pleiotropic effects.

Key words: rice (Oryza sativa L.) , single segment substitution lines , heading date , epistasis

LI Guang-xian, CHEN Ai-hua, LIU Xu, WANG Wen-ying, DING Han-feng, LI Jun, LIU Wei, LI Si-shen , YAO Fang-yin. QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2014, 13(11): 2311-2321.

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QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

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