Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (3): 736-750.DOI: 10.1016/S2095-3119(21)63642-0

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  • 收稿日期:2020-08-20 接受日期:2021-02-01 出版日期:2022-03-01 发布日期:2021-12-06

The comparative analysis and identification of secondary metabolites between Tibet wild and cultivated pomegranates (Punica granatum L.) in China

GUO Lin-hui1, 2, GE Da-peng1, 2, REN Yuan1, 2, DONG Jian-mei1, 2, ZHAO Xue-qing1, 2, LIU Xue-qing3, YUAN Zhao-he1, 2    

  1. 1 Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R.China
    2 College of Forestry, Nanjing Forestry University, Nanjing 210037, P.R.China
    3 Yantai Academy of Agricultural Sciences, Yantai 265500, P.R.China
  • Received:2020-08-20 Accepted:2021-02-01 Online:2022-03-01 Published:2021-12-06
  • About author:GUO Lin-hui, E-mail: glhnl@njfu.edu.cn; Correspondence YUAN Zhao-he, Tel/Fax: +86-25-85427056, E-mail: zhyuan88@hotmail.com
  • Supported by:
    This work was supported by the Tibet Autonomous Region Natural Science Foundation (XZ201019ZRG-153), the Initiative Project for Talents of Nanjing Forestry University (GXL2014070, GXL2018032), the National Natural Science Foundation of China (31901341), the Natural Science Foundation of Jiangsu Province (BK20180768), the Priority Academic Program Development of Jiangsu High Education Institutions (PAPD).

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摘要：

次生代谢物质与植物的营养品质和保健功能密切相关。本研究首次收集了国内23个野生石榴和27个栽培石榴的成熟果实，比较分析了不同种质石榴果皮和果汁中黄酮和鞣质的含量差异，并采用液相色谱-电喷雾串联质谱法（LC-ESI-MS/MS）法测定了果汁高黄酮种质‘则拉4’果皮（ZLP）和果汁（ZLZ）中的次生代谢物质。方差分析（P<0.05）表明，不同石榴种质间的黄酮和鞣质含量存在显著差异。Pearson相关分析表明，影响石榴黄酮和鞣质含量的主要环境因子是纬度和海拔。本研究在‘则拉4’果皮和果汁中共鉴定出279种次生代谢物质，其中227种次生代谢物首次在石榴中发现。通过正交偏最小二乘判别分析法，在ZLP和ZLZ中筛选出了90种差异代谢物。本研究还筛选了8种特异性种质资源（果皮高黄酮，‘军拥3’；果皮低黄酮，‘胭脂红’；果汁高黄酮，‘则拉4’；果汁低黄酮，‘豫大籽’，果皮高鞣质，‘军拥4’，果皮低鞣质，‘安巴1’，果汁高鞣质，‘叶巴1’，果汁低鞣质，‘白花玉石籽’。本研究结果可为我国野生石榴资源的开发利用和石榴育种提供参考。


Abstract: Secondary metabolites are closely related to the nutritional quality and health functions of plants.  We investigated the secondary metabolites of both wild (n=23) and cultivated (n=27) pomegranate plants (Punica granatum L.) growing in China.  The total flavonoid (TF) and tannin (TT) contents from the peel and juice were determined and the secondary metabolites in the peel (ZLP) and juice (ZLZ) of ‘Zela 4’ were identified using liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS).  Analysis of variance (P<0.05) showed that there were significant differences in the TF content of peel (TF (P)) and juice (TF (J)), and the TT content of peel (TT (P)) and juice (TT (J)) among different pomegranate accessions.  Pearson correlation analysis showed that latitude and altitude might be the main environmental factors affecting TF and TT contents in pomegranates.  In this study, 279 secondary metabolites were identified in the ZLP and ZLZ.  In addition, we report for the first time 227 secondary metabolites in pomegranates.  Using orthogonal partial least squares discriminant analysis, 90 differential metabolites were identified in ZLP and ZLZ.  In addition, we screened eight specific germplasms (high-TF (P), ‘Junyong 3’; low-TF (P), ‘Yanzhihong’; high-TF(J), ‘Zela 4’; low-TF (J), ‘Yudazi’, high-TT (P), ‘Junyong 4’; low-TT (P), ‘Anba 1’; high-TT(J), ‘Yeba 1’; and low-TT (J), ‘Baihuayushizi’).  The results of our study provide a reference for the development and utilization of wild pomegranate resources and pomegranate breeding in China.


Key words: wild pomegranate ,  secondary metabolites ,  flavonoid ,  tannin ,  LC-ESI-MS/MS