Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

doi:10.1016/S2095-3119(14)60755-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2577-2583.DOI: 10.1016/S2095-3119(14)60755-3

Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China

收稿日期:2013-11-07 出版日期:2014-12-01 发布日期:2014-12-10
通讯作者: WANG Bao-tong, Mobile: 13572410050, E-mail: wangbt@nwsuaf.edu.cn; LI Qiang, Mobile: 15319489826, E-mail: qiangli@nwsuaf.edu.cn
作者简介:LIU Ze-guang, E-mail: liuzg881111@nwsuaf.edu.cn
基金资助:
The research was supported by the Programme of Introducing Talents of Discipline to Universities, Ministry of Education, China (111 Project, B07049), the National Basic Research Program of China (973 Program, 2013CB127700), the Science and Technology Co-ordinating Innovative Engineering Project of Shaanxi Province, China (2012KTCL02-10), the National Natural Science Foundation of China (30771397), and the China Postdoctoral Science Foundation (2012M512034).

Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China

Received:2013-11-07 Online:2014-12-01 Published:2014-12-10
Contact: WANG Bao-tong, Mobile: 13572410050, E-mail: wangbt@nwsuaf.edu.cn; LI Qiang, Mobile: 15319489826, E-mail: qiangli@nwsuaf.edu.cn
About author:LIU Ze-guang, E-mail: liuzg881111@nwsuaf.edu.cn
Supported by:
The research was supported by the Programme of Introducing Talents of Discipline to Universities, Ministry of Education, China (111 Project, B07049), the National Basic Research Program of China (973 Program, 2013CB127700), the Science and Technology Co-ordinating Innovative Engineering Project of Shaanxi Province, China (2012KTCL02-10), the National Natural Science Foundation of China (30771397), and the China Postdoctoral Science Foundation (2012M512034).

摘要/Abstract

摘要： Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspecific hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene(s) in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, flanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring (CS) nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The flanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.

关键词: Puccinia striiformis f. sp. tritici , Psathyrostachys huashanica Keng , resistance gene , genetic analysis , SSR markers

Abstract: Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspecific hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene(s) in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, flanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring (CS) nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The flanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.

Key words: Puccinia striiformis f. sp. tritici , Psathyrostachys huashanica Keng , resistance gene , genetic analysis , SSR markers

LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue. Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D[J]. Journal of Integrative Agriculture, 2014, 13(12): 2577-2583.

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Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

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