Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

doi:10.1016/S1671-2927(00)8633

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1086-1092.DOI: 10.1016/S1671-2927(00)8633

Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

CHANG Jin-hua, CUI Jiang-hui, XUE Wei, ZHANG Qing-wen

1.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100096, P.R.China
2.College of Agriculture, Agricultural University of Hebei, Baoding 071001, P.R.China
3.Student Employment Guidance Center, Baoding Vocational and Technical College, Baoding 071001, P.R.China

收稿日期:2011-03-18 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: ZHANG Qing-wen, Tel/Fax: +86-10-62733016, E-mail: zhangqingwen@263.net
基金资助:
This work was supported by the Natural Science Foundation, Hebei Province, China (C2010000758) and the Science and Technology Department of Hebei Province, China (06547004D-2).

Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

CHANG Jin-hua, CUI Jiang-hui, XUE Wei, ZHANG Qing-wen

1.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100096, P.R.China
2.College of Agriculture, Agricultural University of Hebei, Baoding 071001, P.R.China
3.Student Employment Guidance Center, Baoding Vocational and Technical College, Baoding 071001, P.R.China

Received:2011-03-18 Online:2012-07-01 Published:2012-07-27
Contact: ZHANG Qing-wen, Tel/Fax: +86-10-62733016, E-mail: zhangqingwen@263.net
Supported by:
This work was supported by the Natural Science Foundation, Hebei Province, China (C2010000758) and the Science and Technology Department of Hebei Province, China (06547004D-2).

摘要/Abstract

摘要： In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid-sensitive pool. 192 pairs of AFLP (amplified fragment length polymorphism) marker primers were screened in these pools using BSA (bulked segregant analysis). Three pairs of EcoR I-CTG/Mse I-CCT, EcoR I-CTG/Mse I-CAT, and EcoR I-AGT/Mse I-CCC showed linkage with aphis resistance. EcoR I-CTG/Mse I-CCT-475, EcoR I-CTG/Mse I-CAT-390, and EcoR I-AGT/Mse I-CCC- 350 (E42/M52-350) were mapped within 6, 10, and 13 cM distances with the aphid-resistant gene by using Mapmaker 3.0 software. The bands amplified by EcoR I-CTG/Mse I-CCT-475 and EcoR I-CTG/Mse I-CAT-390 were extracted, cloned, and sequenced. Specific primers of SCAR (sequence characterized amplified regions) were then designed from these bands. A specific band of 300 bp was amplified by a pair of SCAR primers designed based on the sequence obtained from the EcoR I-CTG/Mse I-CAT-390 marker. The SCAR marker was named SCA50. The marker was used to detect the F2, BC1, and F2:3 populations. The selective efficiency was 86.8, 91.1, and 86.3% in the BC1, F2, and F2:3 populations, respectively. The average selective efficiency was 88.2%.

关键词: sorghum bicolor, aphid resistance gene, molecular marker, SCAR, molecular assistant selection

Abstract: In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid-sensitive pool. 192 pairs of AFLP (amplified fragment length polymorphism) marker primers were screened in these pools using BSA (bulked segregant analysis). Three pairs of EcoR I-CTG/Mse I-CCT, EcoR I-CTG/Mse I-CAT, and EcoR I-AGT/Mse I-CCC showed linkage with aphis resistance. EcoR I-CTG/Mse I-CCT-475, EcoR I-CTG/Mse I-CAT-390, and EcoR I-AGT/Mse I-CCC- 350 (E42/M52-350) were mapped within 6, 10, and 13 cM distances with the aphid-resistant gene by using Mapmaker 3.0 software. The bands amplified by EcoR I-CTG/Mse I-CCT-475 and EcoR I-CTG/Mse I-CAT-390 were extracted, cloned, and sequenced. Specific primers of SCAR (sequence characterized amplified regions) were then designed from these bands. A specific band of 300 bp was amplified by a pair of SCAR primers designed based on the sequence obtained from the EcoR I-CTG/Mse I-CAT-390 marker. The SCAR marker was named SCA50. The marker was used to detect the F2, BC1, and F2:3 populations. The selective efficiency was 86.8, 91.1, and 86.3% in the BC1, F2, and F2:3 populations, respectively. The average selective efficiency was 88.2%.

Key words: sorghum bicolor, aphid resistance gene, molecular marker, SCAR, molecular assistant selection

CHANG Jin-hua, CUI Jiang-hui, XUE Wei, ZHANG Qing-wen. Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers[J]. Journal of Integrative Agriculture, 2012, 12(7): 1086-1092.

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Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

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