Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

doi:10.1016/S2095-3119(13)60566-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (9): 1580-1588.DOI: 10.1016/S2095-3119(13)60566-3

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

SHI Li-hong, ZHANG Na, HU Ya-ya, WEI Xue-jun, YANG Wen-xiang , LIU Da-qun

Department of Plant Pathology, College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2012-12-06 出版日期:2013-09-01 发布日期:2013-09-15
通讯作者: Correspondences YANG Wen-xiang, Tel: +86-312-7528585, Fax: +86-312-7528500, E-mail: wenxiangyang2003@163.com; LIU Da-qun, Tel: +86-312-7528500, Fax: +86-312-7528500, E-mail: ldq@hebau.edu.cn
作者简介:SHI Li-hong, E-mail: shilihong0715@126.com
基金资助:
The authors would like to acknowledge financial support by the Special Fund for Agro-Scientific Research in the Public Interest, China (200903035) and the National Key Basic Research Program of China (2013CB127700).

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

SHI Li-hong, ZHANG Na, HU Ya-ya, WEI Xue-jun, YANG Wen-xiang , LIU Da-qun

Department of Plant Pathology, College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2012-12-06 Online:2013-09-01 Published:2013-09-15
Contact: Correspondences YANG Wen-xiang, Tel: +86-312-7528585, Fax: +86-312-7528500, E-mail: wenxiangyang2003@163.com; LIU Da-qun, Tel: +86-312-7528500, Fax: +86-312-7528500, E-mail: ldq@hebau.edu.cn
About author:SHI Li-hong, E-mail: shilihong0715@126.com
Supported by:
The authors would like to acknowledge financial support by the Special Fund for Agro-Scientific Research in the Public Interest, China (200903035) and the National Key Basic Research Program of China (2013CB127700).

摘要/Abstract

摘要： To detect the leaf rust resistance genes in the 7 Chinese spring wheat clultivars Shenmian 99025, Shenmia 99042, Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 1167 and Shenmian 962, Thatcher, Thatcher backgrounded near-isogenic lines and 15 pathotypes of P. triticina were used for gene postulate at the seedling stage, and 9 of the 15 pathotypes were used in the field tests. Molecular markers closely linked to, or co-segregated with resistance genes Lr1, Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr26, Lr28, Lr29, Lr32, Lr34, Lr35, Lr37, Lr38, and Lr47 were screened to assist detection of the resistance genes. As results, 4 known resistance genes, including Lr1, Lr9, Lr26, and Lr34, and other unknown resistance genes were postulated singly or in combination in the tested cultivars. Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 962, Shenmian 1167, and Shenmian 99042 are potentially useful for wheat production and breeding programs. The result suggested that combining gene postulation, molecular markers and pedigrees is effective and more accuracy method to know the resistance genes in cultivars.

关键词: gene postulation , molecular marker , Triticum aestivum , Puccinia triticina , resistance gene

Abstract: To detect the leaf rust resistance genes in the 7 Chinese spring wheat clultivars Shenmian 99025, Shenmia 99042, Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 1167 and Shenmian 962, Thatcher, Thatcher backgrounded near-isogenic lines and 15 pathotypes of P. triticina were used for gene postulate at the seedling stage, and 9 of the 15 pathotypes were used in the field tests. Molecular markers closely linked to, or co-segregated with resistance genes Lr1, Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr26, Lr28, Lr29, Lr32, Lr34, Lr35, Lr37, Lr38, and Lr47 were screened to assist detection of the resistance genes. As results, 4 known resistance genes, including Lr1, Lr9, Lr26, and Lr34, and other unknown resistance genes were postulated singly or in combination in the tested cultivars. Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 962, Shenmian 1167, and Shenmian 99042 are potentially useful for wheat production and breeding programs. The result suggested that combining gene postulation, molecular markers and pedigrees is effective and more accuracy method to know the resistance genes in cultivars.

Key words: gene postulation , molecular marker , Triticum aestivum , Puccinia triticina , resistance gene

SHI Li-hong, ZHANG Na, HU Ya-ya, WEI Xue-jun, YANG Wen-xiang , LIU Da-qun. Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars[J]. Journal of Integrative Agriculture, 2013, 12(9): 1580-1588.

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Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

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