NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

doi:10.1016/S2095-3119(14)60759-0

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1662-1671.DOI: 10.1016/S2095-3119(14)60759-0

NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

ZHANG Kun, XU Jian-fei, DUAN Shao-guang, PANG Wan-fu, BIAN Chun-song, LIU Jie and JIN

Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, Beijing 100081, P.R.China

收稿日期:2013-04-15 出版日期:2014-08-01 发布日期:2014-08-02
通讯作者: XU Jian-fei, Tel: +86-10-82109543, E-mail: xujianfei@caas.cn; JIN Li-ping, Tel: +86-10-82105943, E-mail: jinliping@caas.cn
基金资助:
This project was supported by the Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, P.R.China and funded by the National Natural Science Foundation of China (NSFC, 31000738).

NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

ZHANG Kun, XU Jian-fei, DUAN Shao-guang, PANG Wan-fu, BIAN Chun-song, LIU Jie and JIN

Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, Beijing 100081, P.R.China

Received:2013-04-15 Online:2014-08-01 Published:2014-08-02
Contact: XU Jian-fei, Tel: +86-10-82109543, E-mail: xujianfei@caas.cn; JIN Li-ping, Tel: +86-10-82105943, E-mail: jinliping@caas.cn
Supported by:
This project was supported by the Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, P.R.China and funded by the National Natural Science Foundation of China (NSFC, 31000738).

摘要/Abstract

摘要： Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates (race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site (NBS) profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region (SCAR) marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.

关键词: potato , late blight , resistance gene , NBS profiling , broad-spectrum resistance

Abstract: Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates (race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site (NBS) profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region (SCAR) marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.

Key words: potato , late blight , resistance gene , NBS profiling , broad-spectrum resistance

ZHANG Kun, XU Jian-fei, DUAN Shao-guang, PANG Wan-fu, BIAN Chun-song, LIU Jie and JIN. NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans[J]. Journal of Integrative Agriculture, 2014, 13(8): 1662-1671.

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NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

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