Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato

doi:10.1016/S2095-3119(13)60369-X

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (9): 1530-1539.DOI: 10.1016/S2095-3119(13)60369-X

Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato

QIN Hua, ZHOU Shuang , ZHANG Yi-zheng

College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China

收稿日期:2012-10-15 出版日期:2013-09-01 发布日期:2013-09-15
通讯作者: Correspondence ZHANG Yi-zheng, Tel/Fax: +86-28-85412738, E-mail: yizzhang@scu.edu.cn
作者简介:QIN Hua, E-mail: qinhua0818@gmail.com
基金资助:
This work was supported by funds from the National Science & Technology Pillar Program of China (2007BAD78B03) and the 11th Five-Year Plan Key Project of Sichuan Province, China (07SG111-003-1).

Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato

QIN Hua, ZHOU Shuang , ZHANG Yi-zheng

College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China

Received:2012-10-15 Online:2013-09-01 Published:2013-09-15
Contact: Correspondence ZHANG Yi-zheng, Tel/Fax: +86-28-85412738, E-mail: yizzhang@scu.edu.cn
About author:QIN Hua, E-mail: qinhua0818@gmail.com
Supported by:
This work was supported by funds from the National Science & Technology Pillar Program of China (2007BAD78B03) and the 11th Five-Year Plan Key Project of Sichuan Province, China (07SG111-003-1).

摘要/Abstract

摘要： Spatial and temporal expression patterns of Sbe1 and Sbe2 that encode starch branching enzyme (SBE) I and II, respectively, in sweet potato (Ipomoea batatas L.) were analyzed. Expression of both genes in Escherichia coli indicate that both genes encoded active SBE. Analysis with real-time quantitative polymerase chain reaction technique indicates that IbSbe1 mRNA was expressed at very low levels in leaves but was the predominant isoform in tuberous root while the reverse case was found for IbSbe2. The expression pattern of IbSbe1, closely resembles that of AGPase S, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. Western analysis detected at least two isoforms of SBE I in tuberous roots, those two isoforms showed adverse expression patterns with the development of the tuberous roots. Expression of the two IbSbe genes exhibited a diurnal rhythm during a 12-h cycle when fed a continuous solution of sucrose. Abscisic acid (ABA) was aother potent inducer of IbSbe expression, but bypassed the semidian oscillator.

关键词: sweet potato , starch branching enzyme , cDNA cloning , cDNA expression , expression patterns , sucrose induction , ABA induction

Abstract: Spatial and temporal expression patterns of Sbe1 and Sbe2 that encode starch branching enzyme (SBE) I and II, respectively, in sweet potato (Ipomoea batatas L.) were analyzed. Expression of both genes in Escherichia coli indicate that both genes encoded active SBE. Analysis with real-time quantitative polymerase chain reaction technique indicates that IbSbe1 mRNA was expressed at very low levels in leaves but was the predominant isoform in tuberous root while the reverse case was found for IbSbe2. The expression pattern of IbSbe1, closely resembles that of AGPase S, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. Western analysis detected at least two isoforms of SBE I in tuberous roots, those two isoforms showed adverse expression patterns with the development of the tuberous roots. Expression of the two IbSbe genes exhibited a diurnal rhythm during a 12-h cycle when fed a continuous solution of sucrose. Abscisic acid (ABA) was aother potent inducer of IbSbe expression, but bypassed the semidian oscillator.

Key words: sweet potato , starch branching enzyme , cDNA cloning , cDNA expression , expression patterns , sucrose induction , ABA induction

QIN Hua, ZHOU Shuang , ZHANG Yi-zheng. Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato[J]. Journal of Integrative Agriculture, 2013, 12(9): 1530-1539.

参考文献

[1]Baguma Y, Sun C, Ahlandsberg S, Mutisya J, Palmqvist S,Rubaihayo P R, Magambo M J, Egwang T G, LarssonH, Jansson C. 2003. Expression patterns of the geneencoding starch branching enzyme II in the storageroots of cassava (Manihot esculenta Crantz). PlantScience, 164, 833- 839.

[2]Baguma Y, Sun C, Borén M, Olsson H, Rosenqvist S,Mutisya J R. Rubaihayo P, Jansson C. 2008. Sugarmediatedsemidian oscilation of gene expression in thecassava storage root regulates starch synthesis. PlantSignaling and Behavior, 3, 439-445

[3]Burton R A, Bewley J D, Smith A M, Bhattacharyya M K,Tatge H, Ring S, Bull V, Hamilton W D O, Martin C.1995. Starch branching enzymes belonging to distinctenzyme families are differentially expressed during peaembryo development. The Plant Journal, 7, 3-15

[4]Church G M, Gilbert W. 1984. Genomic sequencing.Proceedings of the National Academy of Sciences ofthe United States of America, 81, 1991-1995

[5]CIP (International Potato Center). 2010. About sweetpotato.[2012-7-1] http://www.cipotato.org/sweetpotatoFernie A R, Willmitzer L, Trethewey R N. 2002. Sucrose tostarch: a transition in molecular plant physiology.Trends in Plant Science, 7, 35-41

[6]Finkelstein R R, Gibson S I. 2001. ABA and sugarinteractions regulating development: cross-talk orvoices in a crowd? Current Opinions in Plant Biology,5, 26-32

[7]Fulton D C, Edwards A, Pilling E, Robinson H L, Fahy B,Seale R, Kato L, Donald A M, Geigenberger P, Martin C,et al. 2002. Role of granule-bound starch synthase indetermination of amylopectin structure and starchgranule morphology in potato. Journal of BiologicalChemistry, 277, 10834-10841

[8]Gao M, Fisher D K, Kim K N, Shannon J C, Guiltinan M J.1996. Evolutionary conservation and expressionpatterns of maize starch branching enzyme I and IIbgenes suggests isoform specialization. Plant MolecularBiology, 30, 1223-1232

[9]Geigenberger P, Reimholz R, Derting U, Sonnewald U, StittM. 1999. Decreased expression of sucrose phosphatesynthase strongly inhibits the water stress-inducedsynthesis of sucrose in growing potato tubers. ThePlant Journal, 19, 119-129

[10]Geigenberger P, Stitt M. 2000. Diurnal changes in sucrose,nucleotides, starch synthesis, and AGPS transcript ingrowing potato tubers that are suppressed by decreasedexpression of sucrose phosphate synthase. The PlantJournal, 23, 795-806

[11]Guan H P, Baba T, Preiss J. 1994. Expression of branchingenzyme I of maize endosperm in Escherichia coli. PlantPhysiology, 104, 1449-1453

[12]Guan H P, Li P, Imparl-Radosevich J, Preiss J, keeling P.1997. Comparing the properties of Escherichia colibranching enzyme and maize branching enzyme.Archives of Biochemistry and biophysics, 342, 92-98

[13]Guan H P, Preiss J. 1993. Differentiation of the properties of the branching isozymes from maize (Zea mays). PlantPhysiology, 102, 1269-1273

[14]Hamada T, Kim S H, Shimada T. 2006. Starch-branchingenzyme I gene (IbSBEI) from sweet potato (Ipomoeabatatas); molecular cloning and expression analysis.Biotechnology Letter, 28, 1255-1261

[15]Hizukuri S. 1986. Polymodal distribution of the chain lengthsof amylopectins, and its significance. CarbohydrateResearch, 147, 342-347

[16]Hussain H, Mant A, Seale R, Zeeman S, Hinchliffe E,Edwards A, Hylton C, Bornemann S, Smith A M, MartinC, et al. 2003. Three isoforms of isoamylase contributedifferent catalytic properties for the debranching ofpotato glucans. The Plant Cell, 15, 133-149

[17]Jobling S A, Schwall G P, Westcott R J, Sidebottom C M,Debet M, Gidley M J, Jeffcoat R, Safford R. 1999. Aminor form of starch branching enzyme in potato(Solanum tuberosum L.) tubers has a major effect onstarch structure: cloning and characterization of multipleforms of SBE A. The Plant Jounal, 18, 163-171

[18]Kiel J A K W, Vossen J P M J, Venema G. 1987. A generalmethod for the construction of Escherichia coli mutantsby homologous recombination and plasmidsegregation. Molecular and General Genetics, 207,294-301

[19]Kim K N, Guiltinan M J. 1999. Identification of cis-actingelements important for expression of the starchbranchingenzyme I gene in maize endosperm. PlantPhysiology, 121, 225-236

[20]Kim S H, Hamada T, Otani M, Koga H, Shimada T. 2005a.Use of single-leaf cutting in the study of the expressionof starch synthesis and modification genes insweetpotato. Journal of Plant Biotechnology, 7, 123-127

[21]Kim S H, Hamada T, Otani M, Shimada T. 2005b. Cloningand characterization of sweetpotato isoamylase gene(IbIsa1) isolated from tuberous root. Breeding Science,55, 453-458

[22]Kimura M. 1980. A simple method for estimatingevolutionary rates of base substitutions throughcomparative studies of nucleotide sequences. Journalof Molecular Evolution, 16, 111-120

[23]Ko?mann J, Visser R G F, Müller-Röber B, Willmitzer L,Sonnewald U. 1991. Cloning and expression analysisof a potato cDNA that encodes branching enzyme:evidence for co-expression of starch biosyntheticgenes. Molecular and General Genetics, 230, 39- 44.

[24]Larsson C T, Khoshmoodi J, Ek B, Rask L, Larsson H. 1998.Molecular cloning and characterization of starchbranchingenzyme II from potato. Plant MolecularBiology, 37, 505-511

[25]Marshall J, Sidebottom C, Debet M, Martin C, Smith A M,Edwards A. 1996. Identification of the major starchsynthase in the soluble fraction of potato tubers. ThePlant Cell, 8, 1121-1135

[26]Mizuno K, Kobayashi E, Tachibana M, Kawasaki T,Fujimura T, Funane K, Kobayashi M, Baba T. 2001.Characterization of an isoform of rice starch branchingenzyme, RBE4, in developing seeds. Plant CellPhysiology, 42, 349-357

[27]Morell M K, Blennow A, Kosar-Hashemi B, Samuel M S.1997. Differential expression and properties of starchbranching enzyme isoforms in developing wheatendosperm. Plant Physiology, 113, 201-208

[28]Müller-Röber B T, Sonnewald U, Willmitzer L. 1992.Inhibition of ADP-glucose pyrophosphorylase leadsto sugar storing tubers and influences tuber formationand expression of tuber storage protein genes. EMBOJournal, 11, 1229-1238

[29]Nakata P A, Okita T W. 1995. Differential regulation ofADP-glucose pyrophosphorylase in the sink andsource tissues of potato. Plant Physiology, 108, 361-368

[30]Nozaki K, Hamada S, Nakamori T, Ito H, Sagisaka S, YoshidaH, Takeda Y. 2001. Major isoforms of starch branchingenzymes in premature seeds of kidney bean (phaseolusvulgaris L.). Bioscience Biotechnology Biochemistry,65, 1141-1148

[31]Pao W P, Liu L F, Wang S J. 2005. Circadian expressions ofstarch branching enzyme gene in sweet potato leaves.Journal of Genetics and Molecular Biology, 16, 151-157

[32]Pilling E, Smith A M. 2003. Growth ring formation in thestarch granules of potato tubers. Plant Physiology,132, 365-371

[33]Rolland F, Baena-Gonzalez E, Sheen J. 2006. Sugar sensingand signaling in plants: conserved and novelmechanisms. Annual Review of Plant Biology, 57, 675-709

[34]Rook F, Corker F, Card R, Munz G, Smith C, Bevan M W.2001. Impaired sucrose-induction mutants reveal themodulation of sugar-induced starch biosynthetic geneexpression by abscisic acid signaling. Plant Journal,26, 421-433

[35]Safford R, Jobling S A, Sidebottom C M, Westcott R J,Cooke D, Tober K J, Strongitharm B H, Russell A, GidleyM J. 1998. Consequences of antisense RNA inhibitionof starch branching enzyme activity on properties ofpotato starch. Carbohydrate Polymer, 35, 155-168

[36]Smeekens S. 2000. Sugar-induced signal transduction inplants. Annual Review of Plant Physiology and PlantMolecular Biology, 51, 49-81

[37]Sun C, Sathish P, Ahlandsberg S, Jansson C. 1998. The twogenes encoding starch-branching enzymes IIa and IIbare differentially expressed in barley. The PlantPhysiology, 118, 37-49

[38]Sun C, Palmqvist S, Olsson H, Borén M, Ahlandsberg S,Jansson C. 2003. A novel WRKY transcription factor,SUSIBA2, participates in sugar signaling in barley bybinding to the sugar-responsive elements of the iso1promoter. The Plant Cell, 15, 2076-2092

[39]Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA 4:molecular evolutionary genetics analysis (MEGA)software version 4.0. Molecular Biology Evolution,24, 1596-1599

[40]Veramendi J, Fernie A R, Leisse A, Willmitzer L, TretheweyR N. 2002. Potato hexokinase 2 complements transgenicArabidopsis plants deficient in hexokinase 1 but doesnot play a key role in tuber carbohydrate metabolism.Plant Molecular Biology, 49, 491-501

[41]Wang S J, Yeh K W, Tsai C Y. 2001. Regulation of starchgranule-bound starch synthase I gene expression bycircadian clock and sucrose in the source tissue of sweetpotato. Plant Science, 161, 635-644

[42]Yamanouchi H, Nakamura Y. 1992. Organ specificity ofisoforms of starch branching enzyme (Q-enzyme) in rice.Plant Cell Physiology, 33, 985-991

[43]Zrenner R, Salanoubat M, Willmitzer L, Sonnewald U. 1995.Evidence of the crucial role of sucrose synthase forsink strength using transgenic potato plants. The PlantJournal, 7, 97-107

[44]Zrenner R, Willmitzer L, Sonnewald U. 1993. Analysis ofthe expression of potato uridinediphosphoglucosepyrophosphorylase and its inhibition by antisenseRNA. Planta, 190, 247-252.

Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato

Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

[1]	HE Xiu-ting, LIU Cheng-cheng, LI Zhao-qun, ZHANG Zan, LI Guo-qing, LI Fei , DONG Shuang-. Validation of Reference Genes for Quantitative Real-Time PCR in Laodelphax striatellus[J]. Journal of Integrative Agriculture, 2014, 13(4): 811-818.
[2]	ZHANG Kai, WU Zheng-dan, LI Yan-hua, ZHANG Han, WANG Liang-ping, ZHOU Quan-lu, TANG Dao-bin, FU Yu-fan, HE Feng-fa, JIANG Yu-chun, YANG Hang , WANG Ji-chun. ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China[J]. Journal of Integrative Agriculture, 2014, 13(11): 2346-2361.