QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

doi:10.1016/S2095-3119(13)60655-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1835-1844.DOI: 10.1016/S2095-3119(13)60655-3

QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

YU Ma, CHEN Guo-yue, ZHANG Lian-quan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHANG Lian-quan, LIU Ya-xi, LIU Deng-cai, WANG Ji-rui, PU Zhien, ZHANG Li, LAN Xiu-jin, WEI Yu-ming, LIU Chun-ji , ZHENG You-liang

1、Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2、School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, P.R.China
3、Agronomy College, Sichuan Agricultural University, Chengdu 611130, P.R.China
4、CSIRO Plant Industry, St Lucia, QLD 4067, Australia

收稿日期:2013-07-12 出版日期:2014-09-22 发布日期:2014-09-24
通讯作者: ZHENG You-liang, Tel: +86-835-2882007, Fax: +86-835-2883153, E-mail: ylzheng@sicau.edu.cn
作者简介:YU Ma, E-mail: yuwen.0073@hotmail.com
基金资助:
This work was supported by the National Natural Science Foundation of China (31171556, 31171555, 31230053), the National High-Tech R&D Program of China (2011AA100103-02) and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-9).

QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

YU Ma, CHEN Guo-yue, ZHANG Lian-quan, LIU Ya-xi, LIU Deng-cai, WANG Ji-rui, PU Zhien, ZHANG Li, LAN Xiu-jin, WEI Yu-ming, LIU Chun-ji , ZHENG You-liang

1、Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2、School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, P.R.China
3、Agronomy College, Sichuan Agricultural University, Chengdu 611130, P.R.China
4、CSIRO Plant Industry, St Lucia, QLD 4067, Australia

Received:2013-07-12 Online:2014-09-22 Published:2014-09-24
Contact: ZHENG You-liang, Tel: +86-835-2882007, Fax: +86-835-2883153, E-mail: ylzheng@sicau.edu.cn
About author:YU Ma, E-mail: yuwen.0073@hotmail.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31171556, 31171555, 31230053), the National High-Tech R&D Program of China (2011AA100103-02) and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-9).

摘要/Abstract

摘要： Aegiliops tauschii is classified into two subspecies: Ae. tauschii ssp. tauschii and Ae. tauschii ssp. strangulata. Novel genetic variations exist in Ae. tauschii ssp. tauschii that can be utilized in wheat improvement. We synthesized a hexaploid wheat genotype (SHW-L1) by crossing an Ae. tauschii ssp. tauschii accession (AS60) with a tetraploid wheat genotype (AS2255). A population consisting of 171 F8 recombinant inbred lines was developed from SHW-L1 and Chuanmai 32 to identify QTLs associated with agronomic traits. A new genetic map with high density was constructed and used to detect the QTLs for heading date, kernel width, spike length, spikelet number, and thousand kernel weight. A total of 30 putative QTLs were identified for five investigated traits. Thirteen QTLs were located on D genomes of SHW-L1, six of them showed positive effect on agronomic traits. Chromosome region flanked by wPt-6133–wPt-8134 on 2D carried five environment-independent QTLs. Each QTL accounted for more than 10% phenotypic variance. These QTLs were highly consistent across environments and should be used in wheat breeding.

关键词: genetic map , QTL , DArT , agronomic traits , synthetic wheat , Aegilops tauschii ssp. tauschii

Abstract: Aegiliops tauschii is classified into two subspecies: Ae. tauschii ssp. tauschii and Ae. tauschii ssp. strangulata. Novel genetic variations exist in Ae. tauschii ssp. tauschii that can be utilized in wheat improvement. We synthesized a hexaploid wheat genotype (SHW-L1) by crossing an Ae. tauschii ssp. tauschii accession (AS60) with a tetraploid wheat genotype (AS2255). A population consisting of 171 F8 recombinant inbred lines was developed from SHW-L1 and Chuanmai 32 to identify QTLs associated with agronomic traits. A new genetic map with high density was constructed and used to detect the QTLs for heading date, kernel width, spike length, spikelet number, and thousand kernel weight. A total of 30 putative QTLs were identified for five investigated traits. Thirteen QTLs were located on D genomes of SHW-L1, six of them showed positive effect on agronomic traits. Chromosome region flanked by wPt-6133–wPt-8134 on 2D carried five environment-independent QTLs. Each QTL accounted for more than 10% phenotypic variance. These QTLs were highly consistent across environments and should be used in wheat breeding.

Key words: genetic map , QTL , DArT , agronomic traits , synthetic wheat , Aegilops tauschii ssp. tauschii

YU Ma, CHEN Guo-yue, ZHANG Lian-quan, LIU Ya-xi, LIU Deng-cai, WANG Ji-rui, PU Zhien, ZHANG Li, LAN Xiu-jin, WEI Yu-ming, LIU Chun-ji , ZHENG You-liang. QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii[J]. Journal of Integrative Agriculture, 2014, 13(8): 1835-1844.

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QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

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