Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

doi:10.1016/S2095-3119(14)61002-9

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 1992-2001.DOI: 10.1016/S2095-3119(14)61002-9

Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

REN Xiao-li, LIU Tai-guo, LIU Bo, GAO Li, CHEN Wan-quan

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural
Sciences, Beijing 100193, P.R.China

收稿日期:2014-10-29 出版日期:2015-10-09 发布日期:2015-10-12
通讯作者: LIU Tai-guo, E-mail: liutaiguo@caas.cn;CHEN Wan-quan, E-mail: wqchen@ippcaas.cn
基金资助:
The work was financed by the Ministry of Science and Technology of China (2011CB100403, 2013CB127701, 2012BAD19B04 and 2012AA101501), the National Natural Science Foundation of China (31371884), the Ministry of Agriculture of China (CARS-03), and Science & Technology aiding to Xinjiang Uygur Autonomous Region, China (2013911092) during the course of the study.

Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

REN Xiao-li, LIU Tai-guo, LIU Bo, GAO Li, CHEN Wan-quan

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural
Sciences, Beijing 100193, P.R.China

Received:2014-10-29 Online:2015-10-09 Published:2015-10-12
Contact: LIU Tai-guo, E-mail: liutaiguo@caas.cn;CHEN Wan-quan, E-mail: wqchen@ippcaas.cn
Supported by:
The work was financed by the Ministry of Science and Technology of China (2011CB100403, 2013CB127701, 2012BAD19B04 and 2012AA101501), the National Natural Science Foundation of China (31371884), the Ministry of Agriculture of China (CARS-03), and Science & Technology aiding to Xinjiang Uygur Autonomous Region, China (2013911092) during the course of the study.

摘要/Abstract

摘要： Wheat leaf rust (caused by Puccinia triticina) is one of the most important fungal diseases in China. There are tens of winter wheat cultivars which are approved to be released by the government at a national level and more than 100 wheat cultivars at the provincial level. But there is no information about leaf rust (Lr) genes in these cultivars, which makes it difficult for farmers and breeders to select which cultivars they should plant in their fields and use in their breeding programs. The objective of this paper was to identify the leaf rust resistant genes at seedling stage present in the 84 commercial wheat cultivars from China that have been released in the past few years. A set of 20 near isogenic lines with Thatcher background and 6 lines with known Lr genes were used to test the virulence of 12 races of P. triticina (Pt). By comparing the infection types (ITs) produced on the 84 cultivars by the 12 Pt races with the ITs on the differential sets, the Lr genes were postulated. In addition, 8 molecular markers of Lr genes such as Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr26 and Lr29, which are closely linked to or co-segregated with the Lr gene, were used for further validation of the genes in the 84 Chinese winter wheat cultivars. Twelve Lr genes, including Lr1, Lr3, (Lr3bg), (Lr3ka), Lr11, Lr13, Lr14a, Lr16, Lr26, Lr27, Lr30 and Lr31 were postulated to be present either singly or in combinations in these Chinese wheat cultivars. Lr3 and Lr26 were detected most often in the tested cultivars, with frequencies of 51.2 and 38.1%, respectively. No wheat Lr genes were detected in 16 cultivars, and 4 cultivars may carry unknown Lr genes other than those used in this study. Lr9, Lr20, Lr21, Lr24, Lr25 and Lr29 were not present in any of the 84 tested accessions.

关键词: gene postulation , molecular marker , Puccinia triticina , wheat leaf rust

Abstract: Wheat leaf rust (caused by Puccinia triticina) is one of the most important fungal diseases in China. There are tens of winter wheat cultivars which are approved to be released by the government at a national level and more than 100 wheat cultivars at the provincial level. But there is no information about leaf rust (Lr) genes in these cultivars, which makes it difficult for farmers and breeders to select which cultivars they should plant in their fields and use in their breeding programs. The objective of this paper was to identify the leaf rust resistant genes at seedling stage present in the 84 commercial wheat cultivars from China that have been released in the past few years. A set of 20 near isogenic lines with Thatcher background and 6 lines with known Lr genes were used to test the virulence of 12 races of P. triticina (Pt). By comparing the infection types (ITs) produced on the 84 cultivars by the 12 Pt races with the ITs on the differential sets, the Lr genes were postulated. In addition, 8 molecular markers of Lr genes such as Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr26 and Lr29, which are closely linked to or co-segregated with the Lr gene, were used for further validation of the genes in the 84 Chinese winter wheat cultivars. Twelve Lr genes, including Lr1, Lr3, (Lr3bg), (Lr3ka), Lr11, Lr13, Lr14a, Lr16, Lr26, Lr27, Lr30 and Lr31 were postulated to be present either singly or in combinations in these Chinese wheat cultivars. Lr3 and Lr26 were detected most often in the tested cultivars, with frequencies of 51.2 and 38.1%, respectively. No wheat Lr genes were detected in 16 cultivars, and 4 cultivars may carry unknown Lr genes other than those used in this study. Lr9, Lr20, Lr21, Lr24, Lr25 and Lr29 were not present in any of the 84 tested accessions.

Key words: gene postulation , molecular marker , Puccinia triticina , wheat leaf rust

REN Xiao-li, LIU Tai-guo, LIU Bo, GAO Li, CHEN Wan-quan. Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars[J]. Journal of Integrative Agriculture, 2015, 14(10): 1992-2001.

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Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

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