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Characteristics of Good Taste and High Yield Type Japonica Rice in the Lower Reaches of the Yangtze River ZHU TianCi, MA TianFeng, KE Jian, ZHU TieZhong, HE HaiBing, YOU CuiCui, WU ChenYang, WANG GuanJun, WU LiQuan Scientia Agricultura Sinica    2024, 57 (4): 820-830.   DOI: 10.3864/j.issn.0578-1752.2024.04.015 Abstract （95）   HTML （11）    PDF （1590KB）（48）       Knowledge map    Save 【Objective】In order to explore the common agronomic and physiological characteristics of good taste and high yield type japonica rice in the lower reaches of the Yangtze River, the study could provide theoretical basis and technical support for realizing synergistic improvement of rice yield and quality in this region. 【Method】The cultivar screening tests were conducted in 2018 and 2022 using 14 and 13 conventional japonica rice cultivars, respectively, to systematically compare the yield and composition, taste quality and textural characteristics, as well as agronomic and physiological indicators such as biomass, stem non-structural carbohydrates (NSC), and leaf area duration (LAD) among the different cultivars.【Result】The taste value and yield varied across the different cultivars, so through clustering by taste value and yield, they can be further divided into three types: medium taste and low yield (ML), medium taste and high yield (MH), good taste and high yield (GH). The average taste value and yield of GH in two years were 68.5 and 10.2 t·hm-2, respectively, which were 6.8% higher in taste value and 14.6% higher in yield than MH and ML. In terms of performance for yield and quality traits, GH showed the highest grain filling percentage and taste scores compared to MH and ML. In terms of the dry matter accumulation, GH increased the proportion of dry matter accumulation at the early stage of grain filling stage, and showed the highest dry matter accumulation from heading stage to 15 days after heading stage. Further analysis of the causes of dry matter accumulation at this stage revealed that GH significantly increased the NSC remobilization rate while steadily increasing LAD. Correlation analyses showed highly significant positive correlations between grain filling percentage and taste, dry matter accumulation and LAD from heading stage to 15 days after heading stage, and NSC remobilization rate.【Conclusion】 While maintaining a high LAD from heading stage to 15 days after heading stage (100.4 m2·m-2·d), further increasing NSC remobilization rate during grain filling stage (79.9%) to promote the initiation of grain filling, increasing the dry matter accumulation from heading stage to 15 days after heading stage (3.6 t·hm-2), then improving the grain filling percentage (95.4%), and taste (9.6), which are the common characteristics of good taste and high yield type japonica rice in this region. In addition, the development of water and fertilizer management techniques targeting the enhancement of NSC remobilization at the grain filling stage and NSC accumulation at heading stage is expected to further exploit the yield and taste quality potential of the above good taste and high yield type japonica rice. Table and Figures | Reference | Related Articles | Metrics

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Comparison of Heat Tolerance of Maize Hybrids and Their Parental Inbreds with Different Genotypes XU TianJun, LÜ TianFang, LI ZiHao, ZHANG Yong, LIU HongWei, LIU YueE, CAI WanTao, ZHANG RuYang, SONG Wei, XING JinFeng, ZHAO JiuRan, WANG RongHuan Scientia Agricultura Sinica    2024, 57 (2): 403-415.   DOI: 10.3864/j.issn.0578-1752.2024.02.014 Abstract （124）   HTML （12）    PDF （4552KB）（103）       Knowledge map    Save 【Objective】 In recent years, the adverse weather of high temperature and heat damage in the Huang-Huai-Hai maize region of China occurred frequently, which has become an important adverse factor threatening maize production. Study and clarify the effects of high temperature stress on male and female ear characteristics and yield of maize can provide useful guidance for the cultivation and selection of high temperature tolerant maize varieties. 【Method】 The variety of Zhengdan958 (Zheng58×Chang7-2), Xianyu335 (PH6WC×PH4CV), Jingnongke728 (JingMC01×Jing2416), MC812 (JingB547×Jing2416), and their parents were used as the test materials. High temperature stress before and after flowering (from V12 stage to 7 d after silking) were conducted. The effects of high temperature stress on the growth and development of male and female panicles, ASI, pollen activity, yield and yield components of different genotypes of maize hybrids and their parents were studied. 【Result】 High temperature stress before and after anthesis significantly reduced the ear length, rows per ear and grains per row of the tested maize varieties and their parents, and then resulted significant decrease in yield. Compared with the control, the grain number per spike of Zhengdan958, Xianyu335, Jingnongke728 and MC812 decreased by 22.28%, 47.69%, 6.13% and 8.11% respectively under high temperature stress, resulting yield decrease of 9.50%, 50.61%, 3.17% and 5.00% respectively. Among the parental materials, the decrease of rows per panicle, grains per row and yield of Jing2416 under high temperature treatment was the smallest and not significant, while the decrease of PH6WC was the largest. Under high temperature stress, the total number of tassel branches, the length of tassel, the total amount of loose pollen and pollen activity decreased significantly, the silking period of loose pollen was prolonged, and the duration of loose pollen was shortened. Among them, Jingnongke728 had the smallest decline, followed by MC812, showing good heat resistance, while Zhengdan958 had the largest decline in the length of tassel, but the amount of pollen was the largest due to the large number of tassel branches. Xianyu335 has fewer male panicle branches, a large decrease in male panicle length, the least amount of pollen and low activity. Among the parental materials, Jing2416 had a large amount of total loose pollen and strong pollen vitality under high temperature treatment, with the smallest decline, only 4.50 and 3.98 percentage points. Compared with the control, the interval of loose pollen silking (ASI) was prolonged by 1.6d under high temperature stress. The decrease in male spike length is manifested as Zhengdan958>Xianyu335>MC812>Jingnongke728. Zhengdan958 had the largest decrease in male spike length, but had more branches and the largest pollen yield; Xianyu335 has fewer branches of male spikes, a significant decrease in male spike length, the least pollen quantity, and the lowest activity; Jing 2416 has a large amount of loose pollen and strong pollen vitality, with the smallest decrease (only 4.50% and 3.98%). 【Conclusion】 High temperature stress before and after anthesis has a significant impact on the grain yield, male and female ear development process, pollen activity and filament microstructure of the tested maize varieties. Under high temperature stress at this stage, the decline of yield and pollen activity of Jingnongke728 and MC812 is significantly less than Xianyu335, showing higher single ear yield and heat tolerance. By comparing the heat resistance of the parental inbred lines of the tested maize hybrids, it was found that the heat resistance of the paternal inbred lines was better than that of the maternal inbred lines. The male panicle branch and length of the parent material Jing2416 decreased slightly, the amount of pollen was large, the pollen activity was high, the filaments were hairy, the ability to capture pollen was strong, the single panicle yield was high, and the heat resistance was the best. Therefore, in the planting area with frequent high temperature and heat damage, selecting maize varieties such as Jingnongke728 can achieve stable and high yield; and during the maize breeding process, we should pay more attention to the utilizing of the higher temperature resistant inbred such an Jing2416 and then combination higher temperature maize varieties. Table and Figures | Reference | Related Articles | Metrics

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Characteristics of Endophytic Microbial Community Structures in Stems Between Hylocereus undatus and H. polyrhizus ZHOU XinYan, CHEN SiYu, WEI YuFei, ZHU Yu, FENG JunQian, DING DianCao, LU GuiFeng, YANG ShangDong Scientia Agricultura Sinica    2024, 57 (2): 416-428.   DOI: 10.3864/j.issn.0578-1752.2024.02.015 Abstract （90）   HTML （6）    PDF （4336KB）（98）       Knowledge map    Save 【Objective】 The differences of the endophytic microbial community structures between Hylocereus undatus and H. polyrhizus were analyzed, so as so to explore the correlation between the pulp color of H. polyrhizus and the endophytic microbial community composition and their functional microorganisms. 【Method】 Based on high-throughput sequencing technology, the diversity and richness of endophytic microorganisms (bacteria and fungi) in stems between H. undatus and H. polyrhizus were analyzed. Meanwhile, based on LEfSe analysis, the differences of endophytic microorganisms in stems between H. undatus and H. polyrhizus were also investigated.【Result】 The significant differences of the endophytic microbial community structures were found in stems between H. undatus and H. polyrhizus. Meanwhile, the numbers of specific bacterial and fungal OTUs in stems of H. polyrhizus were all higher than those of H. undatus. At the phylum level, the relative abundance ratio of Ascomycota in stems of H. polyrhizus was 1.15 times higher than that of H. undatus. At the genus level, Streptomyces and Penicillium were the highest abundant dominant bacterial and fungal genera in stems of H. polyrhizus, which were 1.24 and 4.27 times higher than those of H. undatus, respectively. In addition, some bacterial genera, such as Lechevalieria, Glycomyces, unclassified_f__Enterobacteriaceae, Actinomadura, and some fungal gerera, such as Talaromyces, unclassified_f__Serendipitaceae, unclassified_c__GS13, unclassified_o__Atractiellales, unclassified_o__Auriculariales were enriched in stems of H. polyrhizus. LEfSe analysis also showed that Promicromonospora and Xylomyces were significant enriched in stems of H. polyrhizus. 【Conclusion】 All above results suggested that the formation of pigment was closely related to the compositions of endophytic microbial community in stems of H. polyrhizus. The bacterial genera, such as Streptomyces, unclassified_f__Enterobacteriaceae, Promicromonospora, and the fungal phylum and genera, such as Ascomycota, Penicillium, Talaromyces and Xylomyces, were all the potential microorganisms in relating to pigment synthesis and metabolic accumulation in stems of H. polyrhizus. Table and Figures | Reference | Related Articles | Metrics

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Changes of Endogenous Hormones and Polyamines During Ovule Development of Stenospermocarpic Seedless Grape ZHU PeiPei, QIN HaoXiang, ZHANG JianXia Scientia Agricultura Sinica    2023, 56 (23): 4789-4800.   DOI: 10.3864/j.issn.0578-1752.2023.23.018 Abstract （99）   HTML （9）    PDF （536KB）（196）       Knowledge map    Save 【Objective】By exploring the effects of endogenous hormones and polyamines on embryo development during the ovule development of seedless grape, this study provided a theoretical basis for promoting embryo development by spraying exogenous hormones before anthesis and adding exogenous hormones into the medium in vitro culture of ovule.【Method】In this study, the European grape (Vitis vinifera L.) variety Jingxiu and its F1 generation stenospermocarpic seedless variety Qinxiu were used as test materials. The content of endogenous hormones and polyamines in ovule of fruit at different developmental stages were compared by high performance liquid chromatography mass spectrometry (HPLCMS) and ultra performance liquid chromatography (UPLC).【Result】The content of IAA, ZT, ZR, and iPAS reached their highest values at 39 DAF (days after flowering) for Jingxiu and 42 DAF for Qinxiu, and the highest values of the former were higher than those of the latter (more than 1.5 times). For the content of JA and SA, Jingxiu started to rise sharply at 36 DAF (2 000 ng∙g-1 and 6 500 ng∙g-1, respectively), and reached the highest values at 39 DAF (6 500 ng∙g-1 and 10 000 ng∙g-1, respectively), and then, which were declining and remaining at a high level at 42-45 DAF, while Qinxiu was declining and remaining at a low level from 36 DAF (3 500 ng∙g-1 and 3 000 ng∙g-1, respectively). The ACC content of Jingxiu was almost 0 ng∙g-1 at 39 DAF, then increased rapidly and reached its highest value (1 200 ng∙g-1) at 45 DAF, while Qinxiu reached its highest value (900 ng∙g-1) at 39 DAF, then declined to 0 ng∙g-1 at 42 DAF, and remained at a very low level. The ABA content of Jingxiu was almost 0 ng∙g-1 from 36 to 45 DAF, while that of Qinxiu rose sharply from 0 ng∙g-1 at 39 DAF to the highest value (900 ng∙g-1) at 42 DAF. The trends of Put (putrescine), Spm (spermine), and Spd (spermidine) were similar for the two varieties, but Jingxiu was consistently higher than Qinxiu. The highest values of Put and Spd were 42 DAF for Jingxiu and 39 DAF for Qinxiu, except for the highest values of Spm, which were all found at 42 DAF. 【Conclusion】 The lower content of growth promoting substances (IAA, CTK, GA3, ACC, JA, SA, Put, Spd, and Spm) and the higher content of growth inhibiting substances (ABA), and lower ratios of (IAA+GA3)/ABA, (IAA+ZT+GA3)/ABA, Spm/PAs, (Spd+Spm)/PAs, and (Spd+Spm)/Put in the ovules of the seedless variety Qinxiu compared with the seeded variety Jingxiu might be one of the main reasons for seedless grape embryo abortion. Therefore, in the process of seedless grape embryo rescue, the embryo abortion could be inhibited by spraying before flowering or adding to media a certain concentration of growth promoting substances. Table and Figures | Reference | Related Articles | Metrics

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The Modification of Gene Editing Vector for Efficient GFPuv Fluorescence Screening and Its Application in Potato Genetic Transformation DU JingYa, CHEN KaiYuan, PU Jin, ZHOU HuiYing, ZHU GuangTao, ZHANG ChunZhi, DU Hui Scientia Agricultura Sinica    2023, 56 (11): 2223-2236.   DOI: 10.3864/j.issn.0578-1752.2023.11.015 Abstract （252）   HTML （27）    PDF （3831KB）（735）       Knowledge map    Save 【Objective】The improvement and innovation of screening markers contributes to the development of transgenic technology, among which the visual screening markers are widely modified for better effect. Recent studies revealed that an enhanced Yellow Green Fluorescent like Protein (eYGFPuv (GFPuv)) obtained by mutation can emit strong and stable green fluorescence under 365 nm UV light irradiation and be easily observed. Constructing the gene editing vector with GFPuv fluorescence screening marker and carrying out experiment application and verifications in potato genetic transformation will provide technical support for the screening of positive transgenic plants in potato transformation, and lay the foundation for using genome editing technology to create potato male sterile lines in the future. 【Method】By using homologous recombination, the GFPuv expression framework and gene editing element Cas9_sgRNA were successively recombined into pCAMBIA2300 vector, and then with this new designed vector the Agrobacterium-mediated transient expression assay was conducted in tobacco plants. Six editing vectors with potato anther development conservative genes were constructed using this modified vector. The A. rhizogenes strains Ar qual and MSU440 harbouring these vectors were transformed into the potato stem segments respectively, and then the A. rhizogenes-induced hairy roots with green fluorescence were observed and counted under the portable UV lamp. The transformation efficiency and editing efficiency of these vectors were analyzed using hairy root transformation system in two different potato genotypes. In the end, the modified vectors were applied to produce transformed potato plants with modifications on target genes. 【Result】A novel gene editing vector pCAMBIA2300MGFPuv-sgRNACas harbouring a GFPuv fluorescence marker was successfully constructed, and the transient transformation in tobacco plants confirmed that the GFPuv expression framework was expressed successfully. The hairy roots with green fluorescence were screened after the transformation with two kinds of A. rhizogenes, and an additional supplement of kanamycin (Kan) significantly increased the proportion of positive fluorescent roots. Although the transformation rates of the two strains were not significantly different, the hairy roots of MSU440 formed faster. Furthermore, the transformation rates and editing rates of editing vectors for six potato anther development conservative genes in two different potato genotypes were the same, but the editing rates of six target sites differed significantly. Potato genetic transformation using the modified vector confirmed that GFPuv fluorescence could be used for the screening of transgenic callus and plants in potato. 【Conclusion】The hairy root transformation system mediated by A. rhizogenes is an essential approach to verifying the efficiency of gene editing, and GFPuv fluorescence can be used in the screening of transgenic plants in potato transformation. Table and Figures | Reference | Related Articles | Metrics

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